Alignments for a candidate for argC in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Alignments for a candidate for argC in Azospirillum brasilense Sp245

Align Probable N-acetyl-gamma-glutamyl-phosphate reductase, chloroplastic; AGPR; N-acetyl-glutamate semialdehyde dehydrogenase; NAGSA dehydrogenase; EC 1.2.1.38 (characterized)
to candidate AZOBR_RS07795 AZOBR_RS07795 N-acetyl-gamma-glutamyl-phosphate reductase

Query= SwissProt::Q93Z70
         (401 letters)



>FitnessBrowser__azobra:AZOBR_RS07795
          Length = 350

 Score =  415 bits (1066), Expect = e-120
 Identities = 206/343 (60%), Positives = 252/343 (73%)

Query: 59  IRIGLLGASGYTGAEIVRLLANHPHFQVTLMTADRKAGQSMESVFPHLRAQKLPTLVSVK 118
           IR+G+LGASGYTGAE+VR+L  HP  ++  +TA+R+AG+ M  VFPHL    LP LV ++
Sbjct: 8   IRVGILGASGYTGAELVRMLLRHPGVEICALTAERQAGKPMAEVFPHLGQFNLPGLVKIE 67

Query: 119 DADFSTVDAVFCCLPHGTTQEIIKELPTALKIVDLSADFRLRNIAEYEEWYGQPHKAVEL 178
           +  +  +DAVFC LPHGTTQE+I  LP  +K+VDLSADFRL + AEY  WYG  H+AVEL
Sbjct: 68  EVAWDKLDAVFCALPHGTTQEVIAGLPRHIKVVDLSADFRLSDPAEYATWYGHEHRAVEL 127

Query: 179 QKEVVYGLTEILREDIKKARLVANPGCYPTTIQLPLVPLLKANLIKHENIIIDAKSGVSG 238
           QKEV YGLTE  R+ ++KAR+VANPGCYPT   L L+PLL   +I+   I+IDAKSGVSG
Sbjct: 128 QKEVAYGLTEFNRQGVRKARVVANPGCYPTCSLLALLPLLMDEMIEPGGIVIDAKSGVSG 187

Query: 239 AGRGAKEANLYSEIAEGISSYGVTRHRHVPEIEQGLSDVAQSKVTVSFTPHLMPMIRGMQ 298
           AGR AK+ NL++E++EG ++YGV  HRH+PEIEQ L   A   VTVSFTPHL+PM RGM 
Sbjct: 188 AGRDAKQQNLFTEVSEGFNAYGVGHHRHMPEIEQELRLAAGRPVTVSFTPHLVPMNRGMM 247

Query: 299 STIYVEMAPGVRTEDLHQQLKTSYEDEEFVKVLDEGVVPRTHNVRGSNYCHMSVFPDRIP 358
           +TIYV MA GV  +DL   L   YE E FV V   G+ P T +VR SN   + VFPDR P
Sbjct: 248 ATIYVRMADGVTADDLRATLTARYESEPFVNVTAAGIAPATRHVRASNQALIGVFPDRTP 307

Query: 359 GRAIIISVIDNLVKGASGQALQNLNIMLGYPETTGLLHQPLFP 401
             AII+SVIDNLVKGASGQA+QN+N+M G  ETTGL   PLFP
Sbjct: 308 RGAIIVSVIDNLVKGASGQAIQNMNVMFGLGETTGLEQAPLFP 350


Lambda     K      H
   0.318    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 437
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 401
Length of database: 350
Length adjustment: 30
Effective length of query: 371
Effective length of database: 320
Effective search space:   118720
Effective search space used:   118720
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate AZOBR_RS07795 AZOBR_RS07795 (N-acetyl-gamma-glutamyl-phosphate reductase)
to HMM TIGR01850 (argC: N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01850.hmm
# target sequence database:        /tmp/gapView.12849.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01850  [M=345]
Accession:   TIGR01850
Description: argC: N-acetyl-gamma-glutamyl-phosphate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
     2e-133  430.8   0.0   2.3e-133  430.6   0.0    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS07795  AZOBR_RS07795 N-acetyl-gamma-glu


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS07795  AZOBR_RS07795 N-acetyl-gamma-glutamyl-phosphate reductase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  430.6   0.0  2.3e-133  2.3e-133       1     345 []       8     350 .]       8     350 .] 0.97

  Alignments for each domain:
  == domain 1  score: 430.6 bits;  conditional E-value: 2.3e-133
                                 TIGR01850   1 ikvaivGasGYtGaeLlrllakHpevevtklvssreagkklsevhphlkglvdlkleeleeeeileead 69 
                                               i+v+i+GasGYtGaeL+r+l +Hp ve+ +l+++r+agk+++ev+phl ++    l ++ee  + ++ d
  lcl|FitnessBrowser__azobra:AZOBR_RS07795   8 IRVGILGASGYTGAELVRMLLRHPGVEICALTAERQAGKPMAEVFPHLGQFNLPGLVKIEEV-AWDKLD 75 
                                               69**********************************************99888777777776.4679** PP

                                 TIGR01850  70 vvflAlphgvsaelvpellekgvkvidlSadfRlkdaevYekwYgkkhekeelleeavYGlpElnreei 138
                                               +vf+Alphg+++e+++  l +++kv+dlSadfRl+d+++Y++wYg++h++ el++e++YGl+E+nr+ +
  lcl|FitnessBrowser__azobra:AZOBR_RS07795  76 AVFCALPHGTTQEVIA-GLPRHIKVVDLSADFRLSDPAEYATWYGHEHRAVELQKEVAYGLTEFNRQGV 143
                                               ***************5.57899*********************************************** PP

                                 TIGR01850 139 kkaklianPGCyaTaalLalaPllkekliepksiivdaksGvSgAGrkasekslfaevnenlkpYkvtk 207
                                               +ka+++anPGCy+T++lLal Pll +++iep  i++daksGvSgAGr a++++lf+ev+e +++Y v +
  lcl|FitnessBrowser__azobra:AZOBR_RS07795 144 RKARVVANPGCYPTCSLLALLPLLMDEMIEPGGIVIDAKSGVSGAGRDAKQQNLFTEVSEGFNAYGVGH 212
                                               ********************************************************************* PP

                                 TIGR01850 208 HrHtpEieqelsklaekkvkvsftphlvpmtrGilatiyaklkkelteeelrklyeevYedepfvrvlk 276
                                               HrH pEieqel+ +a++ v+vsftphlvpm rG++atiy+++++++t+++lr++++++Ye+epfv+v  
  lcl|FitnessBrowser__azobra:AZOBR_RS07795 213 HRHMPEIEQELRLAAGRPVTVSFTPHLVPMNRGMMATIYVRMADGVTADDLRATLTARYESEPFVNVTA 281
                                               ************88888999************************************************* PP

                                 TIGR01850 277 egelPstkavlgsnfvdigvavdeetkrvvvvsaiDNLvKGaagqAvqnlNlmlgfdetegLeklpllp 345
                                               +g +P+t++v++sn++ igv  d++ + +++vs+iDNLvKGa+gqA+qn+N+m+g+ et+gLe+ pl+p
  lcl|FitnessBrowser__azobra:AZOBR_RS07795 282 AGIAPATRHVRASNQALIGVFPDRTPRGAIIVSVIDNLVKGASGQAIQNMNVMFGLGETTGLEQAPLFP 350
                                               ******************************************************************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (345 nodes)
Target sequences:                          1  (350 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01
# Mc/sec: 11.91
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis