Aligments for a candidate for hisG in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Aligments for a candidate for hisG in Azospirillum brasilense Sp245

Align ATP phosphoribosyltransferase; ATP-PRT; ATP-PRTase; EC 2.4.2.17 (uncharacterized)
to candidate AZOBR_RS19500 AZOBR_RS19500 ATP phosphoribosyltransferase catalytic subunit

Query= curated2:Q2W1X8
         (219 letters)



>FitnessBrowser__azobra:AZOBR_RS19500
          Length = 222

 Score =  342 bits (876), Expect = 4e-99
 Identities = 170/216 (78%), Positives = 190/216 (87%)

Query: 1   MSPQDKLVIALPKGRILDEAMPLVRAAGILPEPAFDDPKSRLLRFGTNHPHIDIIRVRSF 60
           +S    LV+ALPKGRIL E  PL+  AGI PE AFDD  SRLLRF TN P++ IIRVRSF
Sbjct: 1   VSASQPLVLALPKGRILKELRPLLAHAGIEPEAAFDDADSRLLRFATNIPNLSIIRVRSF 60

Query: 61  DVATFVAFGAAHLGVAGNDVLMEFDYPEIYAPLDLGIGACRLSVAEPDDLAASDDPRRWS 120
           DVATFVAFGAAHLGVAGNDVLMEFDYPEIYAPLDLGIG CRLSVAEP +L  SDDP RWS
Sbjct: 61  DVATFVAFGAAHLGVAGNDVLMEFDYPEIYAPLDLGIGKCRLSVAEPVELGESDDPSRWS 120

Query: 121 HVRIATKYPEVTKRHFAARGVQAECVKLNGAMELAPSLGLCTRIVDLVSSGATLKANGLK 180
           HVR+ATKYPEVT++HFAARGVQAECVKLNGAMELAP+LGLC RIVDLVS+G+TLKANGL 
Sbjct: 121 HVRVATKYPEVTRKHFAARGVQAECVKLNGAMELAPTLGLCRRIVDLVSTGSTLKANGLV 180

Query: 181 EVEVLAEVTSRLIVNRAALKTRPDEMAGWIEAFRKA 216
           E+E +A+VTSRLIVNRAA KTRP+E++GWI+ FR+A
Sbjct: 181 EIEHIADVTSRLIVNRAAFKTRPEEISGWIDRFREA 216


Lambda     K      H
   0.322    0.138    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 245
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 219
Length of database: 222
Length adjustment: 22
Effective length of query: 197
Effective length of database: 200
Effective search space:    39400
Effective search space used:    39400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

Align candidate AZOBR_RS19500 AZOBR_RS19500 (ATP phosphoribosyltransferase catalytic subunit)
to HMM TIGR00070 (hisG: ATP phosphoribosyltransferase (EC 2.4.2.17))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00070.hmm
# target sequence database:        /tmp/gapView.7345.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00070  [M=183]
Accession:   TIGR00070
Description: hisG: ATP phosphoribosyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    3.2e-56  176.2   0.0    3.7e-56  176.0   0.0    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS19500  AZOBR_RS19500 ATP phosphoribosyl


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS19500  AZOBR_RS19500 ATP phosphoribosyltransferase catalytic subunit
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  176.0   0.0   3.7e-56   3.7e-56       1     183 []       7     194 ..       7     194 .. 0.94

  Alignments for each domain:
  == domain 1  score: 176.0 bits;  conditional E-value: 3.7e-56
                                 TIGR00070   1 lriAlpKGrleeetlkllekaglklskke....erkliasaedeevevlllrakdiptyvekgaadlGi 65 
                                               l +AlpKGr+++e   ll++ag++ ++      +r l + ++ ++++++ +r+ d++t+v+ gaa+lG+
  lcl|FitnessBrowser__azobra:AZOBR_RS19500   7 LVLALPKGRILKELRPLLAHAGIEPEAAFddadSRLLRFATNIPNLSIIRVRSFDVATFVAFGAAHLGV 75 
                                               679*******************996554334449999999999************************** PP

                                 TIGR00070  66 tGkDlleE.seadvvelldlgfgkcklvlAvpees.dvesledlkegkriATkypnltreylekkgvkv 132
                                                G D+l+E ++++++  ldlg+gkc+l++A p e  + ++++  + + r+ATkyp++tr++++ +gv++
  lcl|FitnessBrowser__azobra:AZOBR_RS19500  76 AGNDVLMEfDYPEIYAPLDLGIGKCRLSVAEPVELgESDDPSRWS-HVRVATKYPEVTRKHFAARGVQA 143
                                               ********99999**********************8888888888.89********************* PP

                                 TIGR00070 133 eivkleGavElapllgladaIvDivetGttLrengLkiieeilessarlia 183
                                               e+vkl+Ga+Elap lgl   IvD+v+tG+tL++ngL++ie+i ++++rli+
  lcl|FitnessBrowser__azobra:AZOBR_RS19500 144 ECVKLNGAMELAPTLGLCRRIVDLVSTGSTLKANGLVEIEHIADVTSRLIV 194
                                               *************************************************96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (183 nodes)
Target sequences:                          1  (222 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 5.81
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis