Align propionate--CoA ligase (EC 6.2.1.17) (characterized)
to candidate AZOBR_RS06340 AZOBR_RS06340 acyl-CoA synthetase
Query= metacyc::MONOMER-21334 (629 letters) >FitnessBrowser__azobra:AZOBR_RS06340 Length = 640 Score = 930 bits (2403), Expect = 0.0 Identities = 439/627 (70%), Positives = 515/627 (82%) Query: 3 YQDVYESWKSDPEAFWMEAAKSIDWVEAPSRALDDSNAPLYEWFTDAKVNTCWNAVDRHV 62 Y ++ SDP+ FW EAA+ I W++ + LDDSNAP Y WFT +NTC+NAVDRHV Sbjct: 9 YNQIHARSLSDPDGFWGEAAEDITWIKRWDKVLDDSNAPFYRWFTGGVLNTCYNAVDRHV 68 Query: 63 EAGRGEQTAIIYDSPITHTKRQISYVELRNRVAMLAGALRAKGVEKGDRVIIYMPMIPEA 122 EAGRG Q AIIYDSP+T T + I+Y E +++VA AGALRA+GVEKGDRVI+YMPMIP++ Sbjct: 69 EAGRGAQAAIIYDSPVTKTVQTITYAEFQDQVARFAGALRAQGVEKGDRVILYMPMIPQS 128 Query: 123 LEAMLACARLGAVHSVVFGGFAANELAVRIDDAQPKAIIAASCGLEPGRVVHYKPLLDGA 182 L AMLACARLGAVHSVVFGGFA +ELA RI+D++PKAI++ASCG+EP RVV YKP+LD A Sbjct: 129 LVAMLACARLGAVHSVVFGGFAPHELATRINDSRPKAIVSASCGIEPNRVVKYKPMLDAA 188 Query: 183 IDMAKHKPEFCVIFQREQEVAHLEEGRDYNWHGFQYGVEPAECVPVEGNHPAYILYTSGT 242 I+ A+HKP ++FQR QE A L EGRD +W EPAECVPV P YILYTSGT Sbjct: 189 IEQAEHKPSSVIVFQRPQETATLVEGRDVDWAEAVAKAEPAECVPVAATDPLYILYTSGT 248 Query: 243 TGAPKGVLRPTAGHLVALNWTMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGNTT 302 TG PKGV+R GH VAL WTMKNIYNV+PG+V+WAASDVGWVVGHSYI YGPL+HG TT Sbjct: 249 TGQPKGVIRDNGGHAVALKWTMKNIYNVEPGEVYWAASDVGWVVGHSYIVYGPLLHGCTT 308 Query: 303 IVFEGKPVGTPDAGTFWRVISEHKVKSFFTAPTAIRAVKREDPKGEMLAKYDLSHLKALY 362 +VFEGKPVGTPDAGTFWRVI +HK+ + FTAPTA RA+KREDP E+L KYDLSH +AL+ Sbjct: 309 VVFEGKPVGTPDAGTFWRVIEQHKIGTLFTAPTAFRAIKREDPNAELLKKYDLSHFRALF 368 Query: 363 LAGERADPDTIIWAQKALSVPVIDHWWQTETGWTIAGNPLGIEELPTKLGSPAKAMPGYD 422 LAGER+DPDT+ WA+ L+VPVIDHWWQTETGW I+GNPLG+ P K GS + MPG+D Sbjct: 369 LAGERSDPDTLHWAEDNLNVPVIDHWWQTETGWAISGNPLGVHLFPIKYGSATRPMPGWD 428 Query: 423 VQILDEGGHQMKPGELGAIAVKLPLPPGTLPGLWNAEARFRKSYLEHFPGYYETGDAGMI 482 VQIL+ ++ G++GAI VKLPLPPGTLP LWNA+ R+RKSYL +PGYY+TGDAG I Sbjct: 429 VQILNAENKEVPRGDIGAICVKLPLPPGTLPTLWNADDRYRKSYLSDYPGYYQTGDAGFI 488 Query: 483 DEDGYLYIMARTDDVINVAGHRLSTGGMEEVLAGHEDVAECAVIGVSDDLKGQMPLGFLC 542 D+DGY+YIMARTDD+INVAGHRLSTGGMEEVLA H+DVAECAVIGV+DDLKGQ+PLGFLC Sbjct: 489 DDDGYVYIMARTDDIINVAGHRLSTGGMEEVLASHKDVAECAVIGVADDLKGQVPLGFLC 548 Query: 543 LNNGCNRDHGDVVKEVVKLVRDKIGPVAAFKLAVVVDRLPKTRSGKILRGTMVSIADGKE 602 L G R H ++VKEVV+LVR++IGPVA FK AVVV+RLPKTRSGKILRGTM IAD ++ Sbjct: 549 LKAGVTRPHEEIVKEVVQLVREQIGPVADFKRAVVVERLPKTRSGKILRGTMQKIADNQD 608 Query: 603 YKMPATIDDPAILDEIKVALQSLGYAK 629 YK PATIDDP IL EI ALQSLGYAK Sbjct: 609 YKTPATIDDPGILPEIAEALQSLGYAK 635 Lambda K H 0.319 0.137 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1433 Number of extensions: 55 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 629 Length of database: 640 Length adjustment: 38 Effective length of query: 591 Effective length of database: 602 Effective search space: 355782 Effective search space used: 355782 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory