Alignments for a candidate for dapA in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapA in Azospirillum brasilense Sp245

Align 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 (characterized)
to candidate AZOBR_RS27060 AZOBR_RS27060 dihydrodipicolinate synthase (DapA)

Query= SwissProt::Q57695
         (289 letters)



>FitnessBrowser__azobra:AZOBR_RS27060
          Length = 299

 Score =  161 bits (407), Expect = 2e-44
 Identities = 97/284 (34%), Positives = 163/284 (57%), Gaps = 5/284 (1%)

Query: 4   GVYPAIITPFK-NKEVDFDGLEENINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIEKV 62
           G++ AI+TP   +  VD  GL+  +   ++ G SG+V +G TGE P LS +E K+V+   
Sbjct: 10  GLFSAIVTPLTVDGSVDIAGLKRLVRHQLDAGASGVVPIGGTGEYPALSRDERKRVVAAC 69

Query: 63  VDVVNGRVQVIAGAGSNCTEEAIELSVFAEDVGADAVLSITPYYNKPTQEGLRKHFGKVA 122
           V+   G+  VI G  S   ++A+E        GA AV+++TPYY   TQEG+R +F    
Sbjct: 70  VEEAGGK-PVIPGVLSTGFQDAVEAGRDFAAAGAAAVMTVTPYYAAGTQEGMRAYFRAYR 128

Query: 123 ESINLPIVLYNVPSRTAVNLEPKTVKLLAEEYSNISAVKEANPNLSQVSELIHDA--KIT 180
           ++++LP++LY +P RT V +  + V+ +AE+ S I  +K ++ ++ +  + +  A  KI+
Sbjct: 129 DAVDLPVMLYQIPRRTTVAVTAEGVQAMAEDGS-IIGMKFSSYDMPEFIKTMKYAGDKIS 187

Query: 181 VLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVNYALEGDFEKAREIHYKLFPLMKA 240
           VLSG + L    +ALG +G +   A I PK ++ +   A  G   +A     ++ P++ +
Sbjct: 188 VLSGEEPLFATHVALGARGGVLASATIYPKIWLRIFALARAGKLNEALAEQTQIDPVVNS 247

Query: 241 MFIETNPIPVKTALNMMGRPAGELRLPLCEMSEEHKKILENVLK 284
           +F+ETNP P+K  + M G P G +RLPL   + E  + L+ V +
Sbjct: 248 IFLETNPGPLKAFMAMAGMPVGGVRLPLTAPTAETTEKLQAVAR 291


Lambda     K      H
   0.315    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 196
Number of extensions: 8
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 289
Length of database: 299
Length adjustment: 26
Effective length of query: 263
Effective length of database: 273
Effective search space:    71799
Effective search space used:    71799
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate AZOBR_RS27060 AZOBR_RS27060 (dihydrodipicolinate synthase (DapA))
to HMM TIGR00674 (dapA: 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00674.hmm
# target sequence database:        /tmp/gapView.3669.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00674  [M=286]
Accession:   TIGR00674
Description: dapA: 4-hydroxy-tetrahydrodipicolinate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.4e-70  222.6   0.0    2.8e-70  222.4   0.0    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS27060  AZOBR_RS27060 dihydrodipicolinat


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS27060  AZOBR_RS27060 dihydrodipicolinate synthase (DapA)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  222.4   0.0   2.8e-70   2.8e-70       2     285 ..      11     292 ..      10     293 .. 0.99

  Alignments for each domain:
  == domain 1  score: 222.4 bits;  conditional E-value: 2.8e-70
                                 TIGR00674   2 vltAliTPfkedgsvdfaalekliesqiekgvdaivvvGtTGEsatLsleEkkkvievavelvknrvpv 70 
                                               + +A++TP+  dgsvd+a l++l+++q+++g +++v++G TGE ++Ls +E+k+v+   ve + +  pv
  lcl|FitnessBrowser__azobra:AZOBR_RS27060  11 LFSAIVTPLTVDGSVDIAGLKRLVRHQLDAGASGVVPIGGTGEYPALSRDERKRVVAACVEEAGG-KPV 78 
                                               679************************************************************99.9** PP

                                 TIGR00674  71 iaGtgsnateeaieltkeaeklgvdgvlvvtPyYnkPtqeGlykhfkaiaeevelPiilYnvPsRtgvs 139
                                               i G+ s+  ++a+e  + ++ +g+++v++vtPyY   tqeG+ ++f a  ++v+lP++lY++P Rt+v 
  lcl|FitnessBrowser__azobra:AZOBR_RS27060  79 IPGVLSTGFQDAVEAGRDFAAAGAAAVMTVTPYYAAGTQEGMRAYFRAYRDAVDLPVMLYQIPRRTTVA 147
                                               ********************************************************************* PP

                                 TIGR00674 140 lepetvkrLaeeveivaiKeasgdlervseikaeakedfkvlsGdDaltleilalGakGviSVasnvap 208
                                               ++ e v+++ae+ +i+++K +s d+ ++ + +++a+++++vlsG++ l    +alGa+G +   + + p
  lcl|FitnessBrowser__azobra:AZOBR_RS27060 148 VTAEGVQAMAEDGSIIGMKFSSYDMPEFIKTMKYAGDKISVLSGEEPLFATHVALGARGGVLASATIYP 216
                                               ********************************************************************* PP

                                 TIGR00674 209 kelkemvkaalegdteeareihqkllklfkalfietNPipvKtalallgliekdelRlPLtelseekke 277
                                               k    + + a +g+ +ea+  ++++ ++++ +f+etNP p+K+ +a++g++++  +RlPLt+ + e++e
  lcl|FitnessBrowser__azobra:AZOBR_RS27060 217 KIWLRIFALARAGKLNEALAEQTQIDPVVNSIFLETNPGPLKAFMAMAGMPVG-GVRLPLTAPTAETTE 284
                                               *****************************************************.*************** PP

                                 TIGR00674 278 klkevlke 285
                                               kl++v ++
  lcl|FitnessBrowser__azobra:AZOBR_RS27060 285 KLQAVARH 292
                                               ***99765 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (286 nodes)
Target sequences:                          1  (299 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.94
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis