Alignments for a candidate for metA in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Alignments for a candidate for metA in Azospirillum brasilense Sp245

Align Homoserine O-succinyltransferase; HST; Homoserine transsuccinylase; HTS; EC 2.3.1.46 (characterized)
to candidate AZOBR_RS14010 AZOBR_RS14010 homoserine O-succinyltransferase

Query= SwissProt::G4RES5
         (306 letters)



>FitnessBrowser__azobra:AZOBR_RS14010
          Length = 307

 Score =  363 bits (932), Expect = e-105
 Identities = 162/305 (53%), Positives = 225/305 (73%), Gaps = 2/305 (0%)

Query: 1   MPIRIPDQLPARKTLETEGVVVMDQSRSARQDIRPLQFGLLNLMPNKQRTETQFARLIAS 60
           MPIRIP+ LPA   L+TEGV+VM ++ + RQDIRPL+FGLLNLMP+K RTETQ ARL+ +
Sbjct: 1   MPIRIPNDLPAFTALQTEGVMVMQEADAIRQDIRPLRFGLLNLMPDKIRTETQIARLLGN 60

Query: 61  TPLQIDLTLVRVADPLSKSTPEDYLQNFYSTWEDVRAKKFDGFVVTGAPIANMPFEDVRY 120
           TPLQ++L+L+R+ + + ++T  D++  FY +WED R + FDGF++TGAP+  MPFE+V Y
Sbjct: 61  TPLQVELSLIRITNHVPRNTAADHMSAFYRSWEDARRETFDGFIITGAPVETMPFEEVSY 120

Query: 121 WPEMLEIMDWTQTNVHHTMFICWGAQAALHHLHGVKRYRMEHKAFGVYRHKVLDTRHPFL 180
           W E+  + DWTQ++VH  + ICW AQAA+HH HGV ++ +  KA GV+RH+      P+L
Sbjct: 121 WDELCSVFDWTQSHVHACLNICWAAQAAVHHFHGVPKHLLPRKASGVFRHRNRAPASPYL 180

Query: 181 RGFSDDLAVPVSRYNDIDRQSLSPD--LDILIDSDEVGICMLDDRKYRAAYMLNHLEYDN 238
            G SD + +PVSR+ ++    L P+  L +L+DS E G C+L+D  +R+ +M NH+EYD 
Sbjct: 181 CGLSDGVPIPVSRWTEVREDDLPPESGLRVLLDSPETGPCLLEDAAHRSLHMFNHIEYDT 240

Query: 239 TSLADEYHRDIEAGLDTPLPVNLFPGNDPSRMPENRWRSHAHLLFQNWINEIYQTTPYEL 298
            +L +EY RD+     TP+P   FP +DPS+ PENRWRSHAHLLF NWIN+IYQTTP+EL
Sbjct: 241 DTLRNEYVRDVAKDAATPVPHGYFPDDDPSQPPENRWRSHAHLLFANWINQIYQTTPFEL 300

Query: 299 EKVGT 303
            ++GT
Sbjct: 301 SRIGT 305


Lambda     K      H
   0.321    0.137    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 373
Number of extensions: 17
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 306
Length of database: 307
Length adjustment: 27
Effective length of query: 279
Effective length of database: 280
Effective search space:    78120
Effective search space used:    78120
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate AZOBR_RS14010 AZOBR_RS14010 (homoserine O-succinyltransferase)
to HMM TIGR01001 (metA: homoserine O-succinyltransferase (EC 2.3.1.46))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01001.hmm
# target sequence database:        /tmp/gapView.804.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01001  [M=301]
Accession:   TIGR01001
Description: metA: homoserine O-succinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.2e-107  345.9   0.0   1.4e-107  345.7   0.0    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS14010  AZOBR_RS14010 homoserine O-succi


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS14010  AZOBR_RS14010 homoserine O-succinyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  345.7   0.0  1.4e-107  1.4e-107       1     300 [.       1     300 [.       1     301 [. 0.99

  Alignments for each domain:
  == domain 1  score: 345.7 bits;  conditional E-value: 1.4e-107
                                 TIGR01001   1 mpirvpdelpavellkeenifvmtekrashqdirplevlilnlmpkkietenqllrllsnsplqvditl 69 
                                               mpir+p++lpa   l+ e+++vm e  a+ qdirpl+  +lnlmp+ki+te+q+ rll+n+plqv++ l
  lcl|FitnessBrowser__azobra:AZOBR_RS14010   1 MPIRIPNDLPAFTALQTEGVMVMQEADAIRQDIRPLRFGLLNLMPDKIRTETQIARLLGNTPLQVELSL 69 
                                               9******************************************************************** PP

                                 TIGR01001  70 lridsrkskntpiehlekfykeleevkdrkfdGlivtGapvellefedvayweelkeilewskenvtst 138
                                               +ri++++++nt ++h+  fy+++e+ + + fdG+i+tGapve ++fe+v+yw+el  +++w++++v ++
  lcl|FitnessBrowser__azobra:AZOBR_RS14010  70 IRITNHVPRNTAADHMSAFYRSWEDARRETFDGFIITGAPVETMPFEEVSYWDELCSVFDWTQSHVHAC 138
                                               ********************************************************************* PP

                                 TIGR01001 139 lyicwaaqaalkllygipkrtleeklsGvykhdiv.kedlllrgfddkflaphsryadldeeliaeltd 206
                                               l icwaaqaa++ ++g+pk+ l++k sGv++h+   + ++ l g+ d + +p sr++++ e+++   + 
  lcl|FitnessBrowser__azobra:AZOBR_RS14010 139 LNICWAAQAAVHHFHGVPKHLLPRKASGVFRHRNRaPASPYLCGLSDGVPIPVSRWTEVREDDLPPESG 207
                                               ********************************998799******************************* PP

                                 TIGR01001 207 leilaesdeagvylaaskdernifvtGhpeydketlrqeyvrdvgeglkvdipknyypkddpektpias 275
                                               l +l++s e+g  l+    +r + +  h eyd +tlr+eyvrdv ++   ++p+ y+p+ddp++ p   
  lcl|FitnessBrowser__azobra:AZOBR_RS14010 208 LRVLLDSPETGPCLLEDAAHRSLHMFNHIEYDTDTLRNEYVRDVAKDAATPVPHGYFPDDDPSQPPENR 276
                                               ********************************************************************* PP

                                 TIGR01001 276 wrshanllfanwlnyavyqktpydl 300
                                               wrsha+llfanw+n ++yq+tp++l
  lcl|FitnessBrowser__azobra:AZOBR_RS14010 277 WRSHAHLLFANWIN-QIYQTTPFEL 300
                                               **************.69******98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (301 nodes)
Target sequences:                          1  (307 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 9.93
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis