Alignments for a candidate for trpB in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpB in Azospirillum brasilense Sp245

Align Tryptophan synthase beta chain 1; EC 4.2.1.20 (characterized, see rationale)
to candidate AZOBR_RS00525 AZOBR_RS00525 tryptophan synthase subunit beta

Query= uniprot:P50383
         (425 letters)



>FitnessBrowser__azobra:AZOBR_RS00525
          Length = 405

 Score =  133 bits (334), Expect = 1e-35
 Identities = 117/365 (32%), Positives = 169/365 (46%), Gaps = 56/365 (15%)

Query: 72  IGRPTPLFRAKRLEEYLKTPARIYFKYEGATPTGSHKINTAIPQAYFAKEEGIEHVVTET 131
           +GRP PL+ A+RL E L   A+IYFK E    TG+HKIN  I Q   A+  G + ++ ET
Sbjct: 61  VGRPNPLYYAERLTEKLGG-AKIYFKREELNHTGAHKINNCIGQILLARRMGKKRIIAET 119

Query: 132 GAGQWGTAVALAASMYNMKSTIFMVKVSYEQKPMRRSIMQLYGANVYASPTNLTEYGRKI 191
           GAGQ G A A   ++++M   I+M +    ++      M+L GA V      +T   R +
Sbjct: 120 GAGQHGVATATVCALFDMPCVIYMGETDIARQQPNVFRMKLLGAEV----VPVTSGARTL 175

Query: 192 LETNPQHPGSLGIAMSEAIEYALKN--EFRYLVGSVLD------VVLLHQSVIGQETITQ 243
            +           AM+EA+   + N  +  YL+G+         +V   QSVIG E   Q
Sbjct: 176 KD-----------AMNEALRDWVTNVADTYYLIGTAAGPHPYPAMVRDFQSVIGDEVRVQ 224

Query: 244 L-DLLGEDADILIGCVGGGSNFGGFTYPFI---------------GNKKGKRYIAVS-SA 286
           + +L G   D L+ CVGGGSN  G  +PF+               G  KG    A S + 
Sbjct: 225 MQELEGRLPDSLVACVGGGSNAIGLFHPFLDDPSVQMVAVEAAGHGIDKGPLAHAASITG 284

Query: 287 EIPKFSKGEYKYDFPDSAGLLPLVKMITLGKDYVPPPIYAGGLRYHGVAPTLSLLTKEGI 346
             P    G   Y   D  G +     I+ G D            Y GV P  S L   G 
Sbjct: 285 GRPGVLHGNRTYLLQDEDGQILEGHSISAGLD------------YPGVGPEHSWLHDIGR 332

Query: 347 VEWREYNEREIFEAAKIFIENQGIVPAPESAHAIRAVVDEAIEARKNNERKVIVFNLSGH 406
           VE+    ++E  +A ++    +GI+PA ESAHA+  +V  A +  K++   ++V  LSG 
Sbjct: 333 VEYVSATDQETLDAFQLCARTEGIIPALESAHALAEIVKRAPKLPKDH---LMVLCLSGR 389

Query: 407 GLLDL 411
           G  D+
Sbjct: 390 GDKDI 394


Lambda     K      H
   0.318    0.138    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 354
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 425
Length of database: 405
Length adjustment: 31
Effective length of query: 394
Effective length of database: 374
Effective search space:   147356
Effective search space used:   147356
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate AZOBR_RS00525 AZOBR_RS00525 (tryptophan synthase subunit beta)
to HMM TIGR00263 (trpB: tryptophan synthase, beta subunit (EC 4.2.1.20))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00263.hmm
# target sequence database:        /tmp/gapView.22052.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00263  [M=385]
Accession:   TIGR00263
Description: trpB: tryptophan synthase, beta subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   9.8e-196  636.2   0.0   1.1e-195  636.1   0.0    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS00525  AZOBR_RS00525 tryptophan synthas


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS00525  AZOBR_RS00525 tryptophan synthase subunit beta
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  636.1   0.0  1.1e-195  1.1e-195       1     384 [.      15     400 ..      15     401 .. 0.99

  Alignments for each domain:
  == domain 1  score: 636.1 bits;  conditional E-value: 1.1e-195
                                 TIGR00263   1 gkfgefGGqyvpevllealeelekayekakkdeefkkeleellkeyagrptpltfaknlskklggakiy 69 
                                               g+fg +GG++v+e+l++ + e+ekay++a++d++f+  ++e lk+y+grp pl++a++l++klggakiy
  lcl|FitnessBrowser__azobra:AZOBR_RS00525  15 GHFGIYGGRFVAETLMPLILEVEKAYREARADPAFEGAIREQLKQYVGRPNPLYYAERLTEKLGGAKIY 83 
                                               68******************************************************************* PP

                                 TIGR00263  70 lkredllhtGahkinnalgqallakrlGkkriiaetGaGqhGvatataaallglecevymGaedverqk 138
                                               +kre+l+htGahkinn +gq+lla+r+GkkriiaetGaGqhGvatat++al+++ c++ymG++d++rq+
  lcl|FitnessBrowser__azobra:AZOBR_RS00525  84 FKREELNHTGAHKINNCIGQILLARRMGKKRIIAETGAGQHGVATATVCALFDMPCVIYMGETDIARQQ 152
                                               ********************************************************************* PP

                                 TIGR00263 139 lnvfrmellgakvvpvtsGsktlkdavnealrdWvtsvedthyvlGsavGphPfPeivrefqsvigeev 207
                                               +nvfrm+llga+vvpvtsG +tlkda+nealrdWvt+v+dt+y++G+a+GphP+P++vr+fqsvig+ev
  lcl|FitnessBrowser__azobra:AZOBR_RS00525 153 PNVFRMKLLGAEVVPVTSGARTLKDAMNEALRDWVTNVADTYYLIGTAAGPHPYPAMVRDFQSVIGDEV 221
                                               ********************************************************************* PP

                                 TIGR00263 208 keqilekegrlPdaviacvGGGsnaiGifaafiedeeveligveagGkGidt..ekhaatlskGkeGvl 274
                                               + q++e egrlPd ++acvGGGsnaiG+f++f++d++v++++vea+G+Gid+    haa+++ G++Gvl
  lcl|FitnessBrowser__azobra:AZOBR_RS00525 222 RVQMQELEGRLPDSLVACVGGGSNAIGLFHPFLDDPSVQMVAVEAAGHGIDKgpLAHAASITGGRPGVL 290
                                               ***************************************************97678************* PP

                                 TIGR00263 275 hGaktkllqdedGqieeahsvsaGldypgvgPehaalaetgraeyeaitdeealealkllskeeGiipa 343
                                               hG++t+llqdedGqi e hs+saGldypgvgPeh++l+++gr+ey+++td+e+l+a++l++++eGiipa
  lcl|FitnessBrowser__azobra:AZOBR_RS00525 291 HGNRTYLLQDEDGQILEGHSISAGLDYPGVGPEHSWLHDIGRVEYVSATDQETLDAFQLCARTEGIIPA 359
                                               ********************************************************************* PP

                                 TIGR00263 344 lesshalaaleklapklkkdeivvvnlsGrGdkdletvaka 384
                                               les+hala ++k apkl+kd+++v+ lsGrGdkd+++va++
  lcl|FitnessBrowser__azobra:AZOBR_RS00525 360 LESAHALAEIVKRAPKLPKDHLMVLCLSGRGDKDIFSVAQH 400
                                               **************************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (405 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 10.23
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis