Align Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; Asd; AsdP; EC 2.6.1.1; EC 4.1.1.12 (characterized)
to candidate AZOBR_RS30390 AZOBR_RS30390 aspartate aminotransferase
Query= SwissProt::Q53IZ1 (531 letters) >FitnessBrowser__azobra:AZOBR_RS30390 Length = 543 Score = 783 bits (2023), Expect = 0.0 Identities = 381/524 (72%), Positives = 444/524 (84%) Query: 2 SKDYRSLANLSPFELKDELIKVASGKANRLMLNAGRGNPNFLATTPRRAFFRLGLFAAAE 61 S DY A LSPFELKDELIK+ASG+ NRLMLNAGRGNPNFLAT PRRAFFRLGLFA AE Sbjct: 3 SIDYSRYAKLSPFELKDELIKLASGRENRLMLNAGRGNPNFLATLPRRAFFRLGLFAVAE 62 Query: 62 SELSYSYMTVGVGGLAKLDGIEGRFERFIAEHRDQEGVKFLGKSLSYVRDQLGLDPAAFL 121 +ELS+SYM GVGGL +L+GIEGRFER+++EHRDQEGV FLG++LSYVRDQLGL + FL Sbjct: 63 AELSFSYMPNGVGGLPRLEGIEGRFERYVSEHRDQEGVVFLGRALSYVRDQLGLSGSGFL 122 Query: 122 HEMVDGILGCNYPVPPRMLTVSEQIVRQYIVREMAGGAVPPESVDLFAVEGGTAAMAYIF 181 HEMV+G+LG NYPVPPRML +SE +VR Y+V+EM GG +PP SVDLFAVEGGTAAM YIF Sbjct: 123 HEMVEGVLGANYPVPPRMLAISEDVVRHYLVKEMVGGFLPPGSVDLFAVEGGTAAMTYIF 182 Query: 182 ESLRISGLLKAGDKVAIGMPVFTPYIEIPELAQYDLKEVPIHADPDNGWQYSDAELDKLK 241 S++ +GL++ GDKVAIG+PVFTPYIEIPEL +Y L EV I+ADP GWQY DAELDKLK Sbjct: 183 NSMKQNGLVERGDKVAIGLPVFTPYIEIPELDEYGLTEVAINADPAKGWQYPDAELDKLK 242 Query: 242 DPDVKIFFCVNPSNPPSVKMDQRSLDRVRAIVAEQRPDLLILTDDVYGTFADEFQSLFSV 301 DP VK+FFCVNPSNPPSVKMD RSLDR+ AIV +R DL+ILTDDVYGTFAD F+SLF+V Sbjct: 243 DPAVKVFFCVNPSNPPSVKMDDRSLDRIAAIVKNERKDLIILTDDVYGTFADNFRSLFAV 302 Query: 302 CPRNTLLVYSFSKYFGATGWRLGVIAAHKDNVFDHALSQLPESAKKALDHRYRSLLPDVR 361 CP NT+LVYSFSKYFGATGWRLGVIA HK NV D ++ LPE K ALD RY SL+PDVR Sbjct: 303 CPENTMLVYSFSKYFGATGWRLGVIATHKTNVCDQRIAALPEERKAALDRRYGSLVPDVR 362 Query: 362 SLKFIDRLVADSRVVALNHTAGLSTPQQVQMVLFSLFALMDEADAYKQALKQLIRRREAT 421 L+FIDRLVADSR VALNHTAGLSTPQQVQMVLFSLFALMDE D YK LK++IRRREA Sbjct: 363 GLRFIDRLVADSRTVALNHTAGLSTPQQVQMVLFSLFALMDEQDGYKAELKKVIRRREAA 422 Query: 422 LYRELGMPPLENPNSVNYYTLIDLQNVTCRLYGEAFSQWAVQQSSTGDMLFRVADETGIV 481 LYRELG+P L +PN+V+YYTL+DL+++ +LYG ++ W D+LFR+A ETGIV Sbjct: 423 LYRELGLPTLSDPNAVDYYTLLDLEDIALKLYGPDYAAWVKANFVPNDLLFRIAAETGIV 482 Query: 482 LLPGRGFGSDRPSGRASLANLNEYEYAAIGRALRRLADELYEQY 525 LLPG+GFG+ +P+ R SLANLNEYEYAAIGR+LR +AD+ +E++ Sbjct: 483 LLPGKGFGTLQPAARVSLANLNEYEYAAIGRSLRGMADQSHEEF 526 Lambda K H 0.321 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 909 Number of extensions: 27 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 531 Length of database: 543 Length adjustment: 35 Effective length of query: 496 Effective length of database: 508 Effective search space: 251968 Effective search space used: 251968 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory