Alignments for a candidate for cysE in Pseudomonas stutzeri RCH2

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysE in Pseudomonas stutzeri RCH2

Align serine O-acetyltransferase (EC 2.3.1.30) (characterized)
to candidate GFF1234 Psest_1267 serine O-acetyltransferase

Query= BRENDA::Q0ZB96
         (273 letters)



>FitnessBrowser__psRCH2:GFF1234
          Length = 264

 Score =  272 bits (696), Expect = 5e-78
 Identities = 135/262 (51%), Positives = 178/262 (67%)

Query: 1   MPCEELDIVWNNIKAEARALADCEPMLASFYHATLLKHENLGSALSYMLANKLASPIMPA 60
           MP   +  +WN+++ +A+   D EP LA+ +   +L   + G AL+Y + + LA     +
Sbjct: 1   MPSHSVSALWNDLRNQAQHALDTEPTLATVFRQAILDRPDFGCALAYRIGHALAENADQS 60

Query: 61  IAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPLLYLKGFHALQAYRIGHWL 120
             + +     +   P +  +A  D+QA+ +RDPA D      L+ KGF ALQAYR+GH L
Sbjct: 61  HTLADRFAGIHHHAPRLAEAACSDLQAIVSRDPAFDTALEVFLFSKGFLALQAYRVGHDL 120

Query: 121 WTQGRRALAIFLQNQVSVSFQVDIHPAAKIGRGIMLDHATGIVVGETAVIEDDVSILQSV 180
             +G R LA+F+Q + +    +DI+PA++IG GIMLDH TGIV+GETAV+ DDVSILQ V
Sbjct: 121 QARGERLLAMFIQARCNERLGIDINPASRIGNGIMLDHGTGIVIGETAVVGDDVSILQGV 180

Query: 181 TLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGAKIGAGSVVLQPVPPHTTAAGVP 240
           TLGGTGK  GDRHPK+R GVMIGAGAKILGNIE+G GAK+GAGS+VL PV PHTT  G P
Sbjct: 181 TLGGTGKEGGDRHPKVRSGVMIGAGAKILGNIEIGEGAKVGAGSIVLHPVAPHTTVVGNP 240

Query: 241 ARIVGKPESDKPAMDMDQHFNG 262
           AR VG P   +PA+DMDQ F+G
Sbjct: 241 ARQVGTPRHARPALDMDQSFDG 262


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 320
Number of extensions: 12
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 273
Length of database: 264
Length adjustment: 25
Effective length of query: 248
Effective length of database: 239
Effective search space:    59272
Effective search space used:    59272
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate GFF1234 Psest_1267 (serine O-acetyltransferase)
to HMM TIGR01172 (cysE: serine O-acetyltransferase (EC 2.3.1.30))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01172.hmm
# target sequence database:        /tmp/gapView.27750.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01172  [M=162]
Accession:   TIGR01172
Description: cysE: serine O-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                           Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                           -----------
    1.2e-69  219.6   2.2    1.9e-69  218.9   1.2    1.7  2  lcl|FitnessBrowser__psRCH2:GFF1234  Psest_1267 serine O-acetyltransf


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__psRCH2:GFF1234  Psest_1267 serine O-acetyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ?   -0.8   0.0     0.075     0.075       6      44 ..      18      57 ..      13      71 .. 0.71
   2 !  218.9   1.2   1.9e-69   1.9e-69       3     162 .]      83     242 ..      81     242 .. 0.99

  Alignments for each domain:
  == domain 1  score: -0.8 bits;  conditional E-value: 0.075
                           TIGR01172  6 kavlerDPaaesal.evlllykglhallayrlahalykrk 44
                                        +++l+ +P   +++ + +l  + +   layr+ hal ++ 
  lcl|FitnessBrowser__psRCH2:GFF1234 18 QHALDTEPTLATVFrQAILDRPDFGCALAYRIGHALAENA 57
                                        55666677766655256778889999999***99997654 PP

  == domain 2  score: 218.9 bits;  conditional E-value: 1.9e-69
                           TIGR01172   3 edlkavlerDPaaesalevlllykglhallayrlahalykrklkllarllselvrvltgvdihPaakigrgvliD 77 
                                         +dl+a+++rDPa+++alev+l+ kg+ al+ayr+ h l  r+ +lla++++ + +   g+di Pa++ig+g+++D
  lcl|FitnessBrowser__psRCH2:GFF1234  83 SDLQAIVSRDPAFDTALEVFLFSKGFLALQAYRVGHDLQARGERLLAMFIQARCNERLGIDINPASRIGNGIMLD 157
                                         69************************************************************************* PP

                           TIGR01172  78 hatGvviGetavigddvsiyqgvtLGgtgkekgkRhPtvkegvvigagakvLGnievgenakiGansvvlkdvpa 152
                                         h+tG+viGetav+gddvsi+qgvtLGgtgke g+RhP+v+ gv+igagak+LGnie+ge+ak+Ga+s+vl++v++
  lcl|FitnessBrowser__psRCH2:GFF1234 158 HGTGIVIGETAVVGDDVSILQGVTLGGTGKEGGDRHPKVRSGVMIGAGAKILGNIEIGEGAKVGAGSIVLHPVAP 232
                                         *************************************************************************** PP

                           TIGR01172 153 eatvvGvpar 162
                                         ++tvvG par
  lcl|FitnessBrowser__psRCH2:GFF1234 233 HTTVVGNPAR 242
                                         ********97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (162 nodes)
Target sequences:                          1  (264 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.90
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis