Align Probable adenylyltransferase/sulfurtransferase MoeZ; EC 2.7.7.-; EC 2.8.1.- (characterized)
to candidate GFF1082 Psest_1115 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2
Query= SwissProt::P9WMN7 (392 letters) >FitnessBrowser__psRCH2:GFF1082 Length = 249 Score = 191 bits (484), Expect = 3e-53 Identities = 107/244 (43%), Positives = 145/244 (59%), Gaps = 4/244 (1%) Query: 15 LSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDF 74 LS EE+ RYSR +++ + +DGQ RLK +RVL+IG GGLG+P LYLAAAGVG + + DF Sbjct: 2 LSDEELLRYSRQILLKQVDIDGQLRLKQSRVLIIGLGGLGSPVALYLAAAGVGELHLADF 61 Query: 75 DVVDESNLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQYD 134 D +D +NLQRQ+ H +G K SA + A+NP +R+ H L + + D Sbjct: 62 DTLDLTNLQRQIAHDTPSLGLHKVDSAMARLRALNPHVRLIPHRSGLDADSLDAAIARVD 121 Query: 135 LILDGTDNFATRYLVNDAAVLAGKPYVWGSIYRFEGQASVFWEDAPDGLGVNYRDLYPEP 194 L+LD TDNF R VN A V A KP V G+ R EGQ SVF D Y LY Sbjct: 122 LVLDCTDNFGIREAVNAACVKARKPLVSGAAIRLEGQLSVF--DPRIETSPCYHCLYGHG 179 Query: 195 PPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLLVYDALEMSYRTITI 254 + +C+E GV+G + V S+ EA+KL+ G GE L+GRLL+ DAL +R + + Sbjct: 180 SEAEL--TCSEAGVIGPLVGMVGSLQALEALKLLAGFGEPLVGRLLLIDALSSRFRELRV 237 Query: 255 RKDP 258 ++DP Sbjct: 238 KRDP 241 Lambda K H 0.319 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 241 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 392 Length of database: 249 Length adjustment: 27 Effective length of query: 365 Effective length of database: 222 Effective search space: 81030 Effective search space used: 81030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory