Align imidazole glycerol-phosphate synthase (EC 4.3.2.10) (characterized)
to candidate GFF156 Psest_0156 imidazoleglycerol phosphate synthase, cyclase subunit
Query= BRENDA::Q9SZ30 (592 letters) >FitnessBrowser__psRCH2:GFF156 Length = 256 Score = 144 bits (362), Expect = 5e-39 Identities = 109/312 (34%), Positives = 158/312 (50%), Gaps = 58/312 (18%) Query: 280 LAKRVIACLDVRTNDKGDLVVTKGDQYDVREQSNENEVRNLGKPVDLAGQYYKDGADEIS 339 LAKR+I CLDV D G +V KG Q++ +R+ G PV++A +Y + GADEI+ Sbjct: 3 LAKRIIPCLDV---DNGRVV--KGVQFE--------NIRDAGDPVEIARRYDEQGADEIT 49 Query: 340 FLNITGFRDFPLGDLPMIQVLRQTSKNVFVPLTVGGGIRDFTDASGRYYSSLEVAAEYFR 399 FL+IT D D + V R S+ VF+PLTVGGG+R D Sbjct: 50 FLDITASVDNR--DTTLHTVERMASQ-VFIPLTVGGGVRSVQDIRN-----------LLN 95 Query: 400 SGADKISIGSDAVSAAEEFIKSGVKTGKSSLEQISRVYGNQAVVVSIDPRRVYVNHPDDV 459 +GADK+SI + AV + + + + +G+Q +VV+ID ++V Sbjct: 96 AGADKVSINTAAVFNPD------------FVGEAADRFGSQCIVVAIDAKKV-------- 135 Query: 460 PYKVIRVTNPGPNGEEYAWYQCTVSGGREGRPIGAFELAKAVEELGAGEILLNCIDCDGQ 519 + PG G ++ GGR+ + A AK +E+LGAGEILL +D DG Sbjct: 136 -------SAPGEPGR----WEIFTHGGRKPTGLDAVAWAKKMEDLGAGEILLTSMDQDGV 184 Query: 520 GKGFDIDLVKLISDSVGIPVIASSGAGTPDHFSEVFEKTNASAALAAGIFHRKEVPIQSV 579 G+D+ + + IS+++ IPVIAS G G H ++ + A A LAA IFH E I Sbjct: 185 KSGYDLGVTRAISEALCIPVIASGGVGNLQHLADGILEGKADAVLAASIFHFGEYTIPEA 244 Query: 580 KEHLQEERIEVR 591 K +L I +R Sbjct: 245 KAYLAARGIVMR 256 Lambda K H 0.317 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 360 Number of extensions: 20 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 592 Length of database: 256 Length adjustment: 30 Effective length of query: 562 Effective length of database: 226 Effective search space: 127012 Effective search space used: 127012 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory