Alignments for a candidate for dapB in Pseudomonas stutzeri RCH2

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapB in Pseudomonas stutzeri RCH2

Align dihydrodipicolinate reductase; EC 1.3.1.26 (characterized)
to candidate GFF937 Psest_0966 dihydrodipicolinate reductase

Query= CharProtDB::CH_002128
         (273 letters)



>FitnessBrowser__psRCH2:GFF937
          Length = 268

 Score =  318 bits (816), Expect = 6e-92
 Identities = 167/265 (63%), Positives = 198/265 (74%), Gaps = 1/265 (0%)

Query: 7   RVAIAGAGGRMGRQLIQAALALEGVQ-LGAALEREGSSLLGSDAGELAGAGKTGVTVQSS 65
           R+A+ GA GRMG+ LI+A     G   L AA++R  S+L+G+DAGELA  G+ GV +   
Sbjct: 3   RIAVTGAAGRMGKTLIEAVQQTGGAAGLTAAIDRPDSTLVGADAGELAAIGRLGVPLTGE 62

Query: 66  LDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIGTTGFDEAGKQAIRDAAADIAIVF 125
           L    D+FDV IDFT P  TL +L  CR+ GK MVIGTTGF    K+ +  AA +I IVF
Sbjct: 63  LARAVDEFDVLIDFTHPSVTLKNLEVCRRAGKAMVIGTTGFSPEEKERLAAAAREIPIVF 122

Query: 126 AANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHHRHKVDAPSGTALAMGEAIAHALDKD 185
           AANFSVGVN+ LKLL+ AA+V+GD  DIEIIEAHHRHKVDAPSGTAL MGE IA AL +D
Sbjct: 123 AANFSVGVNLCLKLLDTAARVLGDDVDIEIIEAHHRHKVDAPSGTALRMGEVIAEALGRD 182

Query: 186 LKDCAVYSREGHTGERVPGTIGFATVRAGDIVGEHTAMFADIGERLEITHKASSRMTFAN 245
           L+  AVY REG TG R   TIGFATVRAGD+VG+HT +FA  GER+EITHKASSRMTFA 
Sbjct: 183 LQKVAVYGREGQTGARQRETIGFATVRAGDVVGDHTVLFAADGERVEITHKASSRMTFAK 242

Query: 246 GAVRSALWLSGKESGLFDMRDVLDL 270
           GAVR+ALWL  K +GL+DM+DVL L
Sbjct: 243 GAVRAALWLDEKPAGLYDMQDVLGL 267


Lambda     K      H
   0.319    0.135    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 262
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 273
Length of database: 268
Length adjustment: 25
Effective length of query: 248
Effective length of database: 243
Effective search space:    60264
Effective search space used:    60264
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate GFF937 Psest_0966 (dihydrodipicolinate reductase)
to HMM TIGR00036 (dapB: 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00036.hmm
# target sequence database:        /tmp/gapView.30701.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00036  [M=270]
Accession:   TIGR00036
Description: dapB: 4-hydroxy-tetrahydrodipicolinate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
     4e-107  343.7   2.3   4.5e-107  343.6   2.3    1.0  1  lcl|FitnessBrowser__psRCH2:GFF937  Psest_0966 dihydrodipicolinate r


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__psRCH2:GFF937  Psest_0966 dihydrodipicolinate reductase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  343.6   2.3  4.5e-107  4.5e-107       3     270 .]       3     266 ..       1     266 [. 0.97

  Alignments for each domain:
  == domain 1  score: 343.6 bits;  conditional E-value: 4.5e-107
                          TIGR00036   3 kvavaGaaGrmGrevikavkea.edlelvaalerkgsskqgkDiGelagigkvgvpveddleavkvlaekkadvli 77 
                                        ++av+GaaGrmG+ +i+av+++     l+aa++r++s ++g D+Gela ig++gvp++ +l  +     +++dvli
  lcl|FitnessBrowser__psRCH2:GFF937   3 RIAVTGAAGRMGKTLIEAVQQTgGAAGLTAAIDRPDSTLVGADAGELAAIGRLGVPLTGELARA----VDEFDVLI 74 
                                        89*******************944567********************************99977....9******* PP

                          TIGR00036  78 DfttpeavlenvkialekgvrlVvGTTGfseedlkelkdlaekkgvalviapNfaiGvnlllkllekaakvledv. 152
                                        Dft+p+ +l+n++++ ++g+ +V+GTTGfs e++++l+++a +  +++v+a+Nf++Gvnl lkll +aa+vl+d  
  lcl|FitnessBrowser__psRCH2:GFF937  75 DFTHPSVTLKNLEVCRRAGKAMVIGTTGFSPEEKERLAAAARE--IPIVFAANFSVGVNLCLKLLDTAARVLGDDv 148
                                        *******************************************..***************************8644 PP

                          TIGR00036 153 DiEiiElHHrhKkDaPSGTAlklaeiiakargkdlkeaaveeregltGerkkeeiGiaavRggdvvgehtvlFasd 228
                                        DiEiiE+HHrhK+DaPSGTAl+++e+ia+a g+dl+++av++reg tG+r++e+iG+a+vR+gdvvg+htvlFa d
  lcl|FitnessBrowser__psRCH2:GFF937 149 DIEIIEAHHRHKVDAPSGTALRMGEVIAEALGRDLQKVAVYGREGQTGARQRETIGFATVRAGDVVGDHTVLFAAD 224
                                        **************************************************************************** PP

                          TIGR00036 229 GerleitHkassRaafakGvvrairwledkeekvydledvld 270
                                        Ger+eitHkassR++fakG+vra+ wl +k +++yd++dvl+
  lcl|FitnessBrowser__psRCH2:GFF937 225 GERVEITHKASSRMTFAKGAVRAALWLDEKPAGLYDMQDVLG 266
                                        ****************************************96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (268 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.69
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis