Alignments for a candidate for hcs in Pseudomonas stutzeri RCH2

GapMind for Amino acid biosynthesis

Alignments for a candidate for hcs in Pseudomonas stutzeri RCH2

Align homocitrate synthase (EC 2.3.3.14) (characterized)
to candidate GFF1023 Psest_1056 2-isopropylmalate synthase, bacterial type

Query= BRENDA::D0VY45
         (540 letters)



>FitnessBrowser__psRCH2:GFF1023
          Length = 514

 Score =  434 bits (1116), Expect = e-126
 Identities = 246/513 (47%), Positives = 329/513 (64%), Gaps = 19/513 (3%)

Query: 25  VRILDTTLRDGEQSPGAAMTCVQKLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEE 84
           V I DTTLRDGEQSPGA+MT  +KL  AR L +L VD+IEAGF  AS  DF AVK++A+ 
Sbjct: 7   VIIFDTTLRDGEQSPGASMTAEEKLRIARALERLKVDVIEAGFAIASPGDFAAVKLVADN 66

Query: 85  VGNCVDGNGYVPVITGVSRCNEKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKD 144
           + +          +  ++R  + DI  A EAL  A   R+ TFIATSPIHM+YKLR   D
Sbjct: 67  IKDST--------VCSLARAVDADIERAAEALAGANSGRIHTFIATSPIHMQYKLRMQPD 118

Query: 145 QVLETARNMVKFARSLGCTDIQFGAEDAARSDKEFLYQIFGEVIKAGATTLTIPDTVGIA 204
           QV+E A   VK ARSL C D++F  EDA RS+ +FL +I    I AGA T+ IPDTVG A
Sbjct: 119 QVVEQAVRAVKKARSL-CADVEFSCEDAGRSEIDFLCRIIKAAIDAGARTINIPDTVGYA 177

Query: 205 MPFEYGKLIADIKANTPGIENAIMATHCHNDLGLATANTIEGARYGARQLEVTINGIGER 264
           +P +Y   I  +    P  + A+ + HCHNDLGLA AN++     GARQ+E TING+GER
Sbjct: 178 IPHQYADTIRQLLERIPNADKAVFSVHCHNDLGLAVANSLAAVVAGARQVECTINGLGER 237

Query: 265 AGNASFEEVVMALTCRGIDILGGLHTGINTRHILKTSKMVEKYSGLHLQPHKALVGANAF 324
           AGNA+ EE+VMA+  R    L  +HT I T HIL  S++V   +G  +QP+KA+VGANAF
Sbjct: 238 AGNAALEEIVMAIKTR--QDLINVHTRIETEHILAASRLVSGITGFPVQPNKAIVGANAF 295

Query: 325 LHESGIHQDGMLKHRGTYEIISPEDIGLVRSVGDTIVLGKLSGRQALRNRLEELGYKLKD 384
            HESGIHQDG+LKHR TYEI+S + +G      + +V+GK SGR A R+RL+ELG  L+ 
Sbjct: 296 AHESGIHQDGVLKHRETYEIMSAQSVGW---NANKMVMGKHSGRAAFRSRLDELGIVLEG 352

Query: 385 TEVEGVFWQFKAVAEKKKRITDTDLRALVSNEAFNEQP-IWKLGDLQVTCGTVGFSTATV 443
            E+   F +FK +A+KK  I D DL+ALVS+   +E P  +KL  L+V   T     A +
Sbjct: 353 DELNAAFARFKELADKKHEIFDEDLQALVSDTLADEAPEHFKLASLEVASKTGTIPEAKL 412

Query: 444 KLFSIDGSMHVACSIGTGPVDSAYKAINHIVKEPAKLVKYTLGAITEGIDATATTSVEIS 503
            + S+DG+   A + G+GPVD+ +KAI  + +  A L  Y++ AIT+G D+    +V + 
Sbjct: 413 -VISVDGAERSAQAQGSGPVDATFKAIEAVAESGATLQLYSVNAITQGTDSQGEVTVRLE 471

Query: 504 RGDTNHPVFSGTGGGTDVVVSSVDAYLSALNNM 536
           +G     + +G G  TD+VV+S  AYL+ALN M
Sbjct: 472 KGGR---IVNGNGADTDIVVASAKAYLNALNLM 501


Lambda     K      H
   0.318    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 630
Number of extensions: 22
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 540
Length of database: 514
Length adjustment: 35
Effective length of query: 505
Effective length of database: 479
Effective search space:   241895
Effective search space used:   241895
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis