Align NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 (characterized)
to candidate GFF3068 Psest_3126 Homoserine dehydrogenase
Query= SwissProt::Q5F8J4 (435 letters) >FitnessBrowser__psRCH2:GFF3068 Length = 434 Score = 496 bits (1278), Expect = e-145 Identities = 253/435 (58%), Positives = 321/435 (73%), Gaps = 2/435 (0%) Query: 1 MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCDLSEEKARQICPSAAFV 60 MKPV +G+ GLGTVGGG VL+ NAEEI+RR GR I ++ + A+ + Sbjct: 1 MKPVKVGICGLGTVGGGTFNVLKRNAEEIARRAGRGIEVAQIAT-RHPNAKCDTGATPIT 59 Query: 61 KDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTANKKLLAEYGNEIFPLAEK 120 D F++V ++D+VVEL GG +AKE VLKAIENGKH+VTANK L+A +GNEIF A + Sbjct: 60 ADIFDVVNNPEIDIVVELIGGYTLAKELVLKAIENGKHVVTANKALIAVHGNEIFAKARE 119 Query: 121 QNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGTSNFILSEMREKGSAFADVLK 180 + VIV FEA+VAGGIP+IKA+REGLA NRI +AGIINGT NFIL+EMREKG F DVLK Sbjct: 120 KGVIVAFEASVAGGIPVIKAIREGLAGNRINWLAGIINGTGNFILTEMREKGRTFEDVLK 179 Query: 181 EAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMNFSACYLEGISKLDSRDIKYAEE 240 EAQALGYAEADPTFD+EG DA HK+TI++++AFG P+ F Y EGI++L + D+ YAE Sbjct: 180 EAQALGYAEADPTFDVEGIDAAHKLTILASIAFGIPLQFDKAYTEGIARLTTTDVNYAEA 239 Query: 241 LGYRIKLLGVTRKTGKGIELRVHPTLIPESRLLANVDGVMNAVRVNADMVGETLYYGAGA 300 LGYRIK LGV R+T GIELRVHPTLIP RL+ANV+GVMNAV VN D VG TLYYGAGA Sbjct: 240 LGYRIKHLGVARRTDAGIELRVHPTLIPADRLIANVNGVMNAVMVNGDAVGSTLYYGAGA 299 Query: 301 GALPTASAVVADIIDIARLVEADTAHRVPHLAFQPAQVQAQTILPMDEITSSYYLRVQAK 360 G PTASAVVAD++D+ R + D +RVPHLAFQP + ILP+ S+YYLR+QAK Sbjct: 300 GMEPTASAVVADLVDVTRALTTDPNNRVPHLAFQPDSLSDHPILPISACESAYYLRIQAK 359 Query: 361 DEPGTLGQIAALLAQENVSIEALIQKGVIDQT-TAEIVILTHSTVEKHIKSAIAAIEALD 419 D PG L Q+A++L++ ++IE+++QK +Q ++++TH VE+ I AIAA+E LD Sbjct: 360 DHPGVLAQVASILSERGINIESIMQKEAEEQDGLVPVILVTHRVVEQRIDDAIAALEKLD 419 Query: 420 CVEKPITMIRMESLH 434 V + IR+E L+ Sbjct: 420 DVADKVVRIRVEQLN 434 Lambda K H 0.318 0.135 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 541 Number of extensions: 10 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 435 Length of database: 434 Length adjustment: 32 Effective length of query: 403 Effective length of database: 402 Effective search space: 162006 Effective search space used: 162006 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory