Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate GFF3067 Psest_3125 threonine synthase
Query= BRENDA::Q8YFS0 (463 letters) >FitnessBrowser__psRCH2:GFF3067 Length = 469 Score = 504 bits (1298), Expect = e-147 Identities = 261/462 (56%), Positives = 321/462 (69%), Gaps = 2/462 (0%) Query: 1 MKYVSTRGEAPVLGFSDALLAGLARDGGLYLPQEYPQFTAEQIRALRGKSYVEVALAVLT 60 M+Y+STRG+AP L F D LLAGLA DGGLY+P+ P+FT E+I + G Y E+A V+ Sbjct: 1 MRYISTRGQAPALNFEDVLLAGLASDGGLYVPENLPRFTVEEIASWAGLPYHELAFRVMR 60 Query: 61 PFTGGEIPAADFERMVREAYGTFRHDAVCPLVQTDANEFVLELFHGPTLAFKDVAMQLLA 120 PF G IP ADF++++ E YG F H A+ PL Q + NE+VLELFHGPTLAFKD A+QLL Sbjct: 61 PFVAGSIPDADFKQILEETYGVFAHSAIAPLRQLNGNEWVLELFHGPTLAFKDFALQLLG 120 Query: 121 RMMDYVLAQRGERATIVGATSGDTGGAAIEAFGGRDNTDIFILFPNGRVSPVQQRQMTSS 180 R++DYVLA+RGER I+GATSGDTG AAIE +N DIFIL P+ RVS VQ+RQMT+ Sbjct: 121 RLLDYVLAKRGERVVIMGATSGDTGSAAIEGCRRCENVDIFILHPHQRVSEVQRRQMTTI 180 Query: 181 GFSNVHALSIEGNFDDCQNLVKGMFNDLEFCDALSLSGVNSINWARIMPQVVYYFTAALS 240 N+H ++IEGNFDDCQ +VK F D F L VNSINWARIM Q+VYYF AAL Sbjct: 181 AGDNIHNIAIEGNFDDCQEMVKASFADQAFLKGTRLVAVNSINWARIMAQIVYYFHAALQ 240 Query: 241 LGAPDRAVSFTVPTGNFGDIFAGYVAKRMGLPIEQLIIATNDNDILSRTLESGAYEMRGV 300 LG P R+V+F+VPTGNFGDIFAGY+A+ MGLPI QLI+ATN NDIL R + Y+ + Sbjct: 241 LGGPARSVAFSVPTGNFGDIFAGYLARNMGLPINQLIVATNRNDILHRFMSGNRYDKDTL 300 Query: 301 AQTTSPSMDIQISSNFERLLFEAHGRDAAAVRGLMQGLKQSGGFTISEKPLSAIRSEFSA 360 + SPSMDI +SSNFERLLF+ HGR+ AV L+ G + SG ++ E + R F + Sbjct: 301 HASLSPSMDIMVSSNFERLLFDLHGRNGKAVAELLDGFRASGKLSVEEDRWTEARKLFDS 360 Query: 361 GRSTVDETAATIESVLSKDGYLLDPHSAIGVKVARE-KASGTAPMVVLATAHPAKFPDAV 419 ++T ATI V + G +LDPH+AIGV ARE + S + PMV L TAHP KFPDAV Sbjct: 361 LAVDDEQTCATIAQVFKETGEVLDPHTAIGVHAARECRRSLSIPMVTLGTAHPVKFPDAV 420 Query: 420 -KAACGVEPQLPAWLCDLMQRKESFTVLHNELKIVEEYVRHH 460 KA G LPA L DL +R E TVL NELK V+ +V H Sbjct: 421 EKAGIGQALALPAHLADLFERGERCTVLPNELKAVQAFVGQH 462 Lambda K H 0.320 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 551 Number of extensions: 22 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 463 Length of database: 469 Length adjustment: 33 Effective length of query: 430 Effective length of database: 436 Effective search space: 187480 Effective search space used: 187480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate GFF3067 Psest_3125 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.19948.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-82 264.0 0.0 1.3e-82 263.7 0.0 1.1 1 lcl|FitnessBrowser__psRCH2:GFF3067 Psest_3125 threonine synthase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__psRCH2:GFF3067 Psest_3125 threonine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 263.7 0.0 1.3e-82 1.3e-82 10 336 .. 67 429 .. 58 433 .. 0.91 Alignments for each domain: == domain 1 score: 263.7 bits; conditional E-value: 1.3e-82 TIGR00260 10 vtekdlvdlaegstelfrspkla..eevgaenlyvkelfhgPtlaFKDrglqfvavlltkalelgne..tvlcAt 80 ++++d +++ e + +f+ ++a +++ + n +v+elfhgPtlaFKD++lq ++ ll ++l + e ++++At lcl|FitnessBrowser__psRCH2:GFF3067 67 IPDADFKQILEETYGVFAHSAIAplRQLNG-NEWVLELFHGPTLAFKDFALQLLGRLLDYVLAKRGErvVIMGAT 140 558899999999999999999888899999.99***************************98766655799**** PP TIGR00260 81 sGdtgaaaaealagkanvkvvvLyPkgkispv.keklvtalaenakvlaikGdFDdaqdlvkeifedke...klk 151 sGdtg+aa+e+ ++ +nv++++L P+ ++s v +++t n++ +ai+G+FDd+q++vk+ f d+ + lcl|FitnessBrowser__psRCH2:GFF3067 141 SGDTGSAAIEGCRRCENVDIFILHPHQRVSEVqRRQMTTIAGDNIHNIAIEGNFDDCQEMVKASFADQAflkGTR 215 ********************************99*******************************9887788999 PP TIGR00260 152 lnsvNsinparieaqktyafeiveqlgkespdkvvvpvpsgnfgailkGflekkelglpieklaiaaegaadivr 226 l++vNsin+ari aq++y+f ++ qlg+ + v+++vp gnfg+i++G+l+ +++ lpi+ l +a++ + di++ lcl|FitnessBrowser__psRCH2:GFF3067 216 LVAVNSINWARIMAQIVYYFHAALQLGGPA-RSVAFSVPTGNFGDIFAGYLARNMG-LPINQLIVATNRN-DILH 287 ***************************655.57****************9999999.******9999998.**** PP TIGR00260 227 rflksgdlepkedkeTlstAmdignpsnverale.larrslgnledlke.........................s 275 rf+ + ++ + + ls++mdi +sn+er+l+ l r+++ + +l + lcl|FitnessBrowser__psRCH2:GFF3067 288 RFMSGNRYDKDTLHASLSPSMDIMVSSNFERLLFdLHGRNGKAVAELLDgfrasgklsveedrwtearklfdslA 362 **776655555555*******************98888888888887669************************* PP TIGR00260 276 vsdeeileaikklaeeegyllephtavavaalkklvekg....vs.atadpaKFeevve.altgnkl 336 v de++ +i ++ +e g +l+phta++v a ++ + v+ +ta+p KF+++ve a g+ l lcl|FitnessBrowser__psRCH2:GFF3067 363 VDDEQTCATIAQVFKETGEVLDPHTAIGVHAARECRRSLsipmVTlGTAHPVKFPDAVEkAGIGQAL 429 *******************************999888766665227************977777766 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (469 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01 # Mc/sec: 14.49 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory