Alignments for a candidate for Mt_cysM in Pseudomonas fluorescens GW456-L13

GapMind for Amino acid biosynthesis

Alignments for a candidate for Mt_cysM in Pseudomonas fluorescens GW456-L13

Align [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase (EC 2.5.1.113); O-phosphoserine sulfhydrylase (EC 2.5.1.65) (characterized)
to candidate PfGW456L13_4472 Cysteine synthase (EC 2.5.1.47)

Query= BRENDA::P9WP53
         (323 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4472
          Length = 324

 Score =  166 bits (421), Expect = 5e-46
 Identities = 110/321 (34%), Positives = 169/321 (52%), Gaps = 26/321 (8%)

Query: 10  ALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIEQAEADGLLR 69
           ++GNTPLV + R++PR         V +  K+E RNP  S+K R    MI  AE  G L+
Sbjct: 11  SIGNTPLVQINRIAPRG--------VTILVKIEGRNPGYSVKCRIGANMIWDAEGSGKLK 62

Query: 70  PGATILEPTSGNTGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQIIFSAAEGGS 129
           PG TI+EPTSGNTGI LA  A  +GY+L+  MP + S+ERR++L+  GA+++ +    G 
Sbjct: 63  PGMTIVEPTSGNTGIGLAFVAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEPAKGM 122

Query: 130 NTAVATAKELAATNPS-WVMLYQYGNPANTDSHYCGTGPELLADLP-EITHFVAGLGTTG 187
             A+  A E+ A +PS + M  Q+ NPAN   H   TGPE+  D    +   VAG+GT G
Sbjct: 123 KGAIEKAGEIVAGDPSKYFMPAQFDNPANPAIHEKTTGPEIWNDTDGAVDVLVAGVGTGG 182

Query: 188 TLMGTGRFLREHVAN-VKIVAAEP---------RYGEGV----YALRNMDEGFVPELYDP 233
           T+ G  R+++      +  VA EP           GE +    + ++ +  GFVP+  D 
Sbjct: 183 TITGVSRYIKNTQGKPILSVAVEPVVSPVITQALAGEEIKPSPHKIQGIGAGFVPKNLDL 242

Query: 234 EILTARYSVGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVV 293
            I+     V   ++      L+  EGI  GIS+GA +  A+ +       G+     +++
Sbjct: 243 SIVDRVELVSDEESKAMALRLMQEEGILCGISSGAAMAVAVRLAETPEMQGK--TFVVIL 300

Query: 294 ADAGWKYLSTGAYAGSLDDAE 314
            D+G +YLS+  ++    + E
Sbjct: 301 PDSGERYLSSMLFSDLFTEQE 321


Lambda     K      H
   0.317    0.134    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 297
Number of extensions: 19
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 323
Length of database: 324
Length adjustment: 28
Effective length of query: 295
Effective length of database: 296
Effective search space:    87320
Effective search space used:    87320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis