Alignments for a candidate for hisC in Pseudomonas fluorescens GW456-L13

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisC in Pseudomonas fluorescens GW456-L13

Align histidinol-phosphate aminotransferase; tyrosine/phenylalanine aminotransferase (promiscuous) (EC 2.6.1.1; EC 2.6.1.9) (characterized)
to candidate PfGW456L13_2177 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57)

Query= metacyc::BSU22620-MONOMER
         (360 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177
          Length = 370

 Score =  272 bits (696), Expect = 9e-78
 Identities = 150/356 (42%), Positives = 209/356 (58%), Gaps = 3/356 (0%)

Query: 7   LKQLKPYQPGKPIEAVKSEYGLD--KVVKLASNENPYGCSEAAKEALHHEIQQLALYPDG 64
           ++QL PY PGKP++ +  E  LD   ++KLASNENP G    A  A+   + +L  YPDG
Sbjct: 13  VQQLSPYVPGKPVDELARELDLDPASIIKLASNENPLGAGPKALAAIRDALAELTRYPDG 72

Query: 65  YSAALRTRLSKHLNVSETSLIFGNGSDEIIQIICRAFLNDKTNTVTAAPTFPQYKHNAVI 124
              AL++ L++   V    +  GNGS++I++++ RA+L    N V +   F  Y      
Sbjct: 73  NGFALKSLLAEQCRVELDQVTLGNGSNDILELVARAYLAPGLNAVFSEHAFAVYPIATQA 132

Query: 125 EGAEVREIALRPDGSHDLDAMLEAIDEQTQVVWICSPNNPTGTYTSEGELLAFLERVPSR 184
            GA+ + +  + D  HDL AML AID  T+VV+I +PNNPTGT+     L  FL+ VP  
Sbjct: 133 VGAQAKVVPAK-DWGHDLPAMLAAIDANTRVVFIANPNNPTGTWFDAEALDEFLQDVPEH 191

Query: 185 VLVVLDEAYYEYVTAEDYPETVPLLSKYSNLMILRTFSKAYGLAALRVGYGIADENLIRQ 244
           VLVVLDEAY EY    D P+ +  L+ Y NL++ RTFSKAYGLAALRVGYG++   +   
Sbjct: 192 VLVVLDEAYIEYAEGSDLPDGLDFLAAYPNLLVSRTFSKAYGLAALRVGYGLSTAVVADV 251

Query: 245 IEPAREPFNTSRLGQAAAIAALDDQAFIASCVEQNNAGLQQYYDFAKTHGLKCYPSQTNF 304
           +   R+PFN + L  AAA AAL D+ ++A   + N +G+QQ     +  GL   PS+ NF
Sbjct: 252 LNRVRQPFNVNSLALAAACAALKDEEYLAQSRQLNESGMQQLEAGFRELGLSWIPSKGNF 311

Query: 305 VLIDFKRPADELFQALLEKGYIVRSGNALGFPTSLRITIGTKEQNEEILAILAEIL 360
           + +D  + A  +FQ LL +G IVR     G P  LRITIG   +N   L  L ++L
Sbjct: 312 ICVDLGQVAAPVFQGLLREGVIVRPVANYGMPNHLRITIGLPAENSRFLEALTKVL 367


Lambda     K      H
   0.317    0.134    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 376
Number of extensions: 14
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 360
Length of database: 370
Length adjustment: 30
Effective length of query: 330
Effective length of database: 340
Effective search space:   112200
Effective search space used:   112200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate PfGW456L13_2177 (Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57))
to HMM TIGR01141 (hisC: histidinol-phosphate transaminase (EC 2.6.1.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01141.hmm
# target sequence database:        /tmp/gapView.23195.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01141  [M=349]
Accession:   TIGR01141
Description: hisC: histidinol-phosphate transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
   8.7e-116  372.5   0.0   9.8e-116  372.4   0.0    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177  Biosynthetic Aromatic amino acid


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177  Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  372.4   0.0  9.8e-116  9.8e-116       2     348 ..      11     365 ..      10     366 .. 0.98

  Alignments for each domain:
  == domain 1  score: 372.4 bits;  conditional E-value: 9.8e-116
                                               TIGR01141   2 ekikklepYqpg......arelgek..evvkLnsnEnPfgpsekvkealkeelkk 48 
                                                             + +++l+pY+pg      arel  +   ++kL+snEnP+g  +k+++a++++l++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177  11 PGVQQLSPYVPGkpvdelARELDLDpaSIIKLASNENPLGAGPKALAAIRDALAE 65 
                                                             679***************999999999**************************** PP

                                               TIGR01141  49 lhrYpdpqalelkealakylgveeenillgnGsdelielliraflepgdavlvle 103
                                                             l+rYpd ++++lk+ la+++ ve ++++lgnGs++++el++ra+l pg +++++e
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177  66 LTRYPDGNGFALKSLLAEQCRVELDQVTLGNGSNDILELVARAYLAPGLNAVFSE 120
                                                             ******************************************************* PP

                                               TIGR01141 104 ptysmYevsakiagaevkevplkedgqedleavleaakekvklvflasPnnPtGn 158
                                                             +++++Y++ +++ ga+ k vp+k++g +dl+a+l+a++ ++++vf+a+PnnPtG+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177 121 HAFAVYPIATQAVGAQAKVVPAKDWG-HDLPAMLAAIDANTRVVFIANPNNPTGT 174
                                                             *********************99995.9*************************** PP

                                               TIGR01141 159 llkreeiekvleev.edalVVvDeAYieFsee...asvlellaeypnlvvlrTlS 209
                                                             ++++e + ++l+ v e++lVV+DeAYie++e    ++ l++la ypnl+v+rT+S
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177 175 WFDAEALDEFLQDVpEHVLVVLDEAYIEYAEGsdlPDGLDFLAAYPNLLVSRTFS 229
                                                             **************99**************999999******************* PP

                                               TIGR01141 210 KafgLAglRvGyaianaeiiealekvrapynvsslaleaavaalrdsdkiektve 264
                                                             Ka+gLA+lRvGy++++a ++++l++vr+p+nv+slal+aa+aal+d+++++++ +
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177 230 KAYGLAALRVGYGLSTAVVADVLNRVRQPFNVNSLALAAACAALKDEEYLAQSRQ 284
                                                             ******************************************************* PP

                                               TIGR01141 265 evkkererlleelkkleglevyeSkaNFvlikvkedaeelleallekgiivRdlk 319
                                                              +++++++l++++++l gl++++Sk+NF+ +++ + a+ +++ ll++g+ivR ++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177 285 LNESGMQQLEAGFREL-GLSWIPSKGNFICVDLGQVAAPVFQGLLREGVIVRPVA 338
                                                             ****************.8************************************* PP

                                               TIGR01141 320 saeglleeclRitvGtreenerllealke 348
                                                             ++ g+ +++lRit+G + en r+leal +
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2177 339 NY-GM-PNHLRITIGLPAENSRFLEALTK 365
                                                             **.96.********************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (349 nodes)
Target sequences:                          1  (370 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 9.93
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis