Align O-succinylhomoserine sulfhydrylase; OSH sulfhydrylase; OSHS sulfhydrylase; EC 2.5.1.- (characterized)
to candidate PfGW456L13_3934 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)
Query= SwissProt::P55218 (403 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3934 Length = 403 Score = 683 bits (1763), Expect = 0.0 Identities = 335/403 (83%), Positives = 374/403 (92%) Query: 1 MTQDWDAGRLDSDLEGAAFDTLAVRAGQRRTPEGEHGEALFTTSSYVFRTAADAAARFAG 60 M+QDWDAGRLDSDLEG AFDTLAVRAGQ RTPEGEHG+ +F TSSYVFRTAADAAARFAG Sbjct: 1 MSQDWDAGRLDSDLEGVAFDTLAVRAGQHRTPEGEHGDPMFFTSSYVFRTAADAAARFAG 60 Query: 61 EVPGNVYSRYTNPTVRTFEERIAALEGAEQAVATASGMSAILALVMSLCSSGDHVLVSRS 120 EVPGNVYSRYTNPTVR FEERIAALE AEQAVATA+GM+AI+A+VMSLCS+GDHVLVSRS Sbjct: 61 EVPGNVYSRYTNPTVRAFEERIAALESAEQAVATATGMAAIMAVVMSLCSAGDHVLVSRS 120 Query: 121 VFGSTISLFDKYFKRFGIQVDYPPLSDLAAWEAACKPNTKLFFVESPSNPLAELVDIAAL 180 VFGSTISLF+KYFKRFGI+VDY PL+DL+ W+AA K NTKL FVESPSNPLAELVDIA L Sbjct: 121 VFGSTISLFEKYFKRFGIEVDYVPLADLSGWDAAIKANTKLLFVESPSNPLAELVDIAEL 180 Query: 181 AEIAHAKGALLAVDNCFCTPALQQPLKLGADVVIHSATKYIDGQGRGMGGVVAGRGEQMK 240 A+IAHAKGA+L VDNCFCTPALQQPLK+GAD+V+HSATK+IDGQGR MGGVVAGRGEQMK Sbjct: 181 AKIAHAKGAMLVVDNCFCTPALQQPLKMGADIVVHSATKFIDGQGRCMGGVVAGRGEQMK 240 Query: 241 EVVGFLRTAGPTLSPFNAWLFLKGLETLRIRMQAHSASALALAEWLERQPGIERVYYAGL 300 EVVGFLRTAGPTLSPFNAW+FLKGLETL +RM+AH A+A LAEWLE+Q GIE+V+YAGL Sbjct: 241 EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMKAHCANAQQLAEWLEQQDGIEKVHYAGL 300 Query: 301 PSHPQHELARRQQSGFGAVVSFDVKGGRDAAWRFIDATRMVSITTNLGDTKTTIAHPATT 360 SHPQHELA RQQ GFGAVVSF+VKGG++ AWRFIDATR++SIT NLGD+KTTI HP+TT Sbjct: 301 KSHPQHELALRQQKGFGAVVSFEVKGGKEGAWRFIDATRLISITANLGDSKTTITHPSTT 360 Query: 361 SHGRLSPEDRARAGIGDSLIRVAVGLEDLDDLKADMARGLAAL 403 SHGRL+P++R AGI DSLIRVAVGLED+ DL+AD+ARGLAAL Sbjct: 361 SHGRLAPQEREAAGIRDSLIRVAVGLEDVADLQADLARGLAAL 403 Lambda K H 0.319 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 556 Number of extensions: 11 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 403 Length of database: 403 Length adjustment: 31 Effective length of query: 372 Effective length of database: 372 Effective search space: 138384 Effective search space used: 138384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory