Align Carbamoyl-phosphate synthase small chain; EC 6.3.5.5; Carbamoyl-phosphate synthetase glutamine chain (uncharacterized)
to candidate Pf1N1B4_2547 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85)
Query= curated2:Q8KGA2 (366 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2547 Length = 197 Score = 86.7 bits (213), Expect = 5e-22 Identities = 62/180 (34%), Positives = 90/180 (50%), Gaps = 15/180 (8%) Query: 189 NIIRQLNAEGCKVTVV-NAKTTADEVLAMNPDGIFLSNGPGDPFAVTYAIDTIRELAARN 247 N+++ L G +V VV N + T E+ A+NP+ I +S GP P +I+ I+ A + Sbjct: 14 NVVQYLGELGSEVKVVRNDELTIAEIEALNPERIVVSPGPCTPTEAGISIEAIKHFAGK- 72 Query: 248 STLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLLSNTIE------ITSQNHGF 301 LPI G+CLGHQ + AFG + + HG PV + E ++ H Sbjct: 73 --LPILGVCLGHQSIGQAFGGDVVRARQVMHGKTSPVFHEDKGVFEGLNRPLTVTRYHSL 130 Query: 302 AVEMESLPGELELT-HKNLYDMTVE---GIRHRELPCFSVQYHPEAAPGPHDSHYLFKEF 357 V+ E+LP LELT L D +V+ G+RH+ L VQ+HPE+ H LF F Sbjct: 131 IVKRETLPDCLELTAWTQLEDGSVDEIMGLRHKTLNIEGVQFHPESIL-TEQGHELFANF 189 Lambda K H 0.317 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 182 Number of extensions: 9 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 366 Length of database: 197 Length adjustment: 25 Effective length of query: 341 Effective length of database: 172 Effective search space: 58652 Effective search space used: 58652 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory