GapMind for Amino acid biosynthesis

 

Aligments for a candidate for lysZ in Pseudomonas fluorescens FW300-N1B4

Align Putative [LysW]-aminoadipate/[LysW]-glutamate kinase; EC 2.7.2.- (uncharacterized)
to candidate Pf1N1B4_2121 Acetylglutamate kinase (EC 2.7.2.8)

Query= curated2:A0RWW1
         (266 letters)



>lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2121 Acetylglutamate
           kinase (EC 2.7.2.8)
          Length = 301

 Score =  128 bits (321), Expect = 2e-34
 Identities = 80/259 (30%), Positives = 142/259 (54%), Gaps = 16/259 (6%)

Query: 2   ITIKIGGSIVDS--LHPSAIPDIKKAAAGGV--VLVHGGGKEVTKVCEQLGKEPRFVTSP 57
           + IK GG+ ++S  L      DI    A G+  V+VHGGG ++  + ++L  E  F+   
Sbjct: 30  LVIKYGGNAMESEELKTGFARDIVLMKAVGINPVVVHGGGPQIGDLLKRLSIESHFIDG- 88

Query: 58  GNIKSRYTDKETAEIFTMVMSGRINKCIVRMLQQHGVNAVGLSGIDGGLIRAERKSRLVI 117
                R TD +T ++  MV+ G++NK IV ++ +HG +A+GL+G D  LIRA++ +    
Sbjct: 89  ----MRVTDAQTMDVVEMVLGGQVNKDIVNLINRHGGSAIGLTGKDAELIRAKKLTVTRQ 144

Query: 118 VNERGRKQAIEGGYTGRITSVNSELLETLLGKGIVPVVSPIAMGNEYELLNVDGDRAAAN 177
             E  + + I+ G+ G +  +N++LL  L+    +PV++PI +G   E  N++ D  A  
Sbjct: 145 TPEMTQPEIIDIGHVGEVVGINTDLLNLLVKGDFIPVIAPIGVGANGESYNINADLVAGK 204

Query: 178 IAGATKSERILFVTDVDGLMMDEKLVSRLSAAEAEEIRPKIGP-----GMEKKVLAATEA 232
           +A A K+E+++ +T++ GLM  +K  + L+    +++   I       GM  K+  A EA
Sbjct: 205 VAEALKAEKLMLLTNIAGLM--DKSGTVLTGLTTQQVDDLIADGTIYGGMLPKIRCALEA 262

Query: 233 LKMGVREAIIARGSRENPV 251
           ++ GV  ++I  G   N +
Sbjct: 263 VQGGVGSSLIIDGRVPNAI 281


Lambda     K      H
   0.316    0.135    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 191
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 266
Length of database: 301
Length adjustment: 26
Effective length of query: 240
Effective length of database: 275
Effective search space:    66000
Effective search space used:    66000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory