Alignments for a candidate for glnA in Pseudomonas fluorescens FW300-N1B4

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Pseudomonas fluorescens FW300-N1B4

Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate Pf1N1B4_2254 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)

Query= BRENDA::P36205
         (439 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2254
          Length = 426

 Score =  186 bits (473), Expect = 9e-52
 Identities = 130/435 (29%), Positives = 213/435 (48%), Gaps = 32/435 (7%)

Query: 25  INGTLKNLEITPDVFLESWEDGIMF---------DGSSIEGF---VRIEESDMYLKPVLD 72
           +NG ++   I      + +E GI           +GS++E     + I ++D    P+ D
Sbjct: 1   MNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDIGDADRICYPIPD 60

Query: 73  TFAVLPWTVDGAKSARVICDVYTPDGKPFEGDPRYRLRRMMEKAEQLGYTPYAGPEMEFF 132
           T    PW      +A+++  ++  +G PF  DPR  LR+++ K +++G T  A  E+EF+
Sbjct: 61  TLCNEPW--QKRPTAQLLMTMHELEGDPFFADPREVLRQVVAKFDEMGLTICAAFELEFY 118

Query: 133 ILPI-NEKGEPVPEFLDHGG-------YFDLLPLSKVEEIRRDIAIALEKMGITVEATHH 184
           ++   N  G P P      G        + +  L +  +  +DI    ++ GI  +A   
Sbjct: 119 LIDQENVNGRPQPPRSPISGKRPHSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVK 178

Query: 185 EVAPSQHEVDFRYDTF-LRTADNAQTVKLVIKTMAIFHGYHATFMPKPFYGVNGSGMHVH 243
           E AP+Q EV+  +    ++  D A  +K +IK +A  H    TFM KP+ G  G+G+HVH
Sbjct: 179 ESAPAQFEVNLHHVADPIKACDYAVLLKRLIKNIAYDHEMDTTFMAKPYPGQAGNGLHVH 238

Query: 244 MSLFRGDKNAFYDPDDPLGLSKELRYFVGGILKHAKALAAVTNPTINSYKRLVPGYEAPV 303
           +S+   D    +  +DP   +  LR+ +GG+L+   A  A   P +NSY+R    +  P 
Sbjct: 239 ISILDKDGKNIFASEDP-EQNAALRHAIGGVLETLPAQMAFLCPNVNSYRRFGAQFYVPN 297

Query: 304 YISWSVGNRSALIRIPKARGKATRLEYRSPDPSCNIYLAFAAILAAGLDGIINKIEPPAP 363
              W + NR+  IR+P     A R+E+R      N YL  A++LA    G+ NKIEP AP
Sbjct: 298 SPCWGLDNRTVAIRVPTGSSDAVRIEHRVAGADANPYLLMASVLAGVHHGLTNKIEPGAP 357

Query: 364 VEENIYHMTSERREELNIESLPGSLKEAVEELKKDDVIIDALGEHIFEKFVEAAEKDWKE 423
           VE N Y        E N +SLP +L++A+ EL   +V+   +     + FV   E + +E
Sbjct: 358 VEGNSY--------EQNEQSLPNNLRDALRELDDSEVMAKYIDPKYIDIFVACKESELEE 409

Query: 424 FSTYVTNWELQRYLY 438
           F   +++ E   YL+
Sbjct: 410 FEHSISDLEYNWYLH 424


Lambda     K      H
   0.320    0.139    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 473
Number of extensions: 19
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 439
Length of database: 426
Length adjustment: 32
Effective length of query: 407
Effective length of database: 394
Effective search space:   160358
Effective search space used:   160358
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis