GapMind for Amino acid biosynthesis

 

L-isoleucine biosynthesis in Pseudomonas fluorescens FW300-N1B4

Best path

ilvA, ilvI, ilvH, ilvC, ilvD, ilvE

Also see fitness data for the top candidates

Rules

Overview: Isoleucine biosynthesis in GapMind is based on MetaCyc pathways L-isoleucine biosynthesis I (from threonine) (link), II via citramalate (link), or IV from propanoate (link). These pathways share a common intermediate, 2-oxobutanoate, but vary in how the 2-oxobutanoate is formed. Pathway IV is included because propanoate is a common fermentative end product and need not be a nutrient requirement, but it is not always clear if it could be the main pathway to isoleucine. Pathway III (link), via glutamate mutase, is not included because the first step (glutamate mutase, EC 5.4.99.1) has not been linked to sequence and because no organism has been demonstrated to rely on this pathway to form oxobutanoate. Pathway V, from 2-methylbutanoate (link), is not included.

13 steps (10 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
ilvA threonine deaminase Pf1N1B4_2280 Pf1N1B4_4257
ilvI acetohydroxybutanoate synthase regulatory subunit Pf1N1B4_2879
ilvH acetohydroxybutanoate synthase catalytic subunit Pf1N1B4_2878 Pf1N1B4_696
ilvC 2-hydroxy-3-ketol-acid reductoisomerase Pf1N1B4_2880
ilvD (R)-2,3-dihydroxy-3-methylpentanoate dehydratase Pf1N1B4_2303 Pf1N1B4_4621
ilvE isoleucine transaminase Pf1N1B4_4476 Pf1N1B4_27
Alternative steps:
cimA (R)-citramalate synthase
leuB 3-methylmalate dehydrogenase Pf1N1B4_12 Pf1N1B4_4412
leuC citramalate isomerase large subunit Pf1N1B4_15 Pf1N1B4_3821
leuD citramalate isomerase small subunit Pf1N1B4_14 Pf1N1B4_3821
ofoa 2-oxobutanoate:ferredoxin oxidoreductase, alpha subunit
ofob 2-oxobutanoate:ferredoxin oxidoreductase, beta subunit
prpE propionyl-CoA synthetase Pf1N1B4_3430 Pf1N1B4_2849

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory