Alignments for a candidate for ilvA in Pseudomonas fluorescens FW300-N1B4

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvA in Pseudomonas fluorescens FW300-N1B4

Align L-threonine dehydratase biosynthetic IlvA; EC 4.3.1.19; Threonine deaminase (uncharacterized)
to candidate Pf1N1B4_4758 Threonine dehydratase biosynthetic (EC 4.3.1.19)

Query= curated2:Q02145
         (416 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758
          Length = 337

 Score =  231 bits (588), Expect = 3e-65
 Identities = 133/311 (42%), Positives = 188/311 (60%), Gaps = 8/311 (2%)

Query: 17  VVTKTPLQLDPYLSNKYQANIYLKEENLQKVRSFKLRGAYYSISKLSDEQRSKGVVCASA 76
           +  +TPLQ  P LS      I LK E+LQ   SFK+RGAY  +  LSDE +++GV+ ASA
Sbjct: 26  LAVRTPLQAAPALSEALGNQILLKREDLQPTFSFKIRGAYNKLVNLSDEHKARGVITASA 85

Query: 77  GNHAQGVAFAANQLNISATIFMPVTTPNQKISQVKFFGESHVTIRLIGDTFDESARAAKA 136
           GNHAQGVA AA +L I ATI MP TTP  K+  V+  G   +   L G++F  +   A +
Sbjct: 86  GNHAQGVALAARELGIGATIVMPCTTPELKVHGVRSRGAEAL---LHGESFPFALEYALS 142

Query: 137 FSQDNDKPFIDPFDDENVIAGQGTVALEIFAQAKKQGISLDKIFVQIGGGGLIAGITAYS 196
            +Q   + F+ PFDD +VIAGQGTVA+EI  Q   QG  LD IFV +GGGGLIAGI AY+
Sbjct: 143 LAQQTGRTFVSPFDDPDVIAGQGTVAMEILRQ--HQG-PLDAIFVPVGGGGLIAGIAAYA 199

Query: 197 KERYPQTEIIGVEAKGATSMKAAYSAGQPVTLEHIDKFADGIAVATVGQKTYQLINDKVK 256
           K   P+  IIGVE++ +  ++AA  AG+ V L  +  FADG+AVA +G   +++    V 
Sbjct: 200 KYLRPEIRIIGVESEHSACLQAALRAGERVVLPTVGTFADGVAVAQIGAYGFEICRFCVD 259

Query: 257 QLLAVDEGLISQTILELYSKLGIVAEPAGATSVAALE--LIKDEIKGKNIVCIISGGNND 314
           ++L V    +   I  +Y     + EP+GA +VA ++  + +   +G+ +V I SG N +
Sbjct: 260 EVLTVSNDELCAAIKNIYDDTRSITEPSGALAVAGIKQYVARTGARGQTLVAIDSGANIN 319

Query: 315 ISRMQEIEERA 325
              ++ + ERA
Sbjct: 320 FDSLRHVAERA 330


Lambda     K      H
   0.317    0.135    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 320
Number of extensions: 14
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 416
Length of database: 337
Length adjustment: 30
Effective length of query: 386
Effective length of database: 307
Effective search space:   118502
Effective search space used:   118502
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate Pf1N1B4_4758 (Threonine dehydratase biosynthetic (EC 4.3.1.19))
to HMM TIGR01124 (ilvA: threonine ammonia-lyase, biosynthetic (EC 4.3.1.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01124.hmm
# target sequence database:        /tmp/gapView.20695.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01124  [M=499]
Accession:   TIGR01124
Description: ilvA_2Cterm: threonine ammonia-lyase, biosynthetic
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                      Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                      -----------
   2.1e-153  497.4   0.7   2.5e-153  497.2   0.7    1.0  1  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758  Threonine dehydratase biosynthet


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758  Threonine dehydratase biosynthetic (EC 4.3.1.19)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  497.2   0.7  2.5e-153  2.5e-153       2     320 ..      14     332 ..      13     335 .. 0.99

  Alignments for each domain:
  == domain 1  score: 497.2 bits;  conditional E-value: 2.5e-153
                                      TIGR01124   2 ylrailkarvyeaavetplekaaklserlknrvllkredlqpvfsfklrGaynkmaqlsaeqka 65 
                                                    y+++il a vye av tpl+ a  lse l+n++llkredlqp fsfk+rGaynk+ +ls+e+ka
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758  14 YVKKILAAPVYELAVRTPLQAAPALSEALGNQILLKREDLQPTFSFKIRGAYNKLVNLSDEHKA 77 
                                                    899************************************************************* PP

                                      TIGR01124  66 kGviaasaGnhaqGvalsakklGvkavivmpettpeikvdavkafGgevvlhGenydeakakal 129
                                                    +Gvi+asaGnhaqGval+a++lG+ a+ivmp+ttpe+kv+ v+++G+e +lhGe++  a ++al
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758  78 RGVITASAGNHAQGVALAARELGIGATIVMPCTTPELKVHGVRSRGAEALLHGESFPFALEYAL 141
                                                    **************************************************************** PP

                                      TIGR01124 130 elaqekgltfiapfddplviaGqGtvalellrqveedldavfvpvGGGGliaGvaalvkqllpe 193
                                                     laq+ g tf++pfddp+viaGqGtva+e+lrq++ +lda+fvpvGGGGliaG+aa+ k l+pe
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758 142 SLAQQTGRTFVSPFDDPDVIAGQGTVAMEILRQHQGPLDAIFVPVGGGGLIAGIAAYAKYLRPE 205
                                                    **************************************************************** PP

                                      TIGR01124 194 ikvigveaedsaalkqaleaGervkldqvGlfadGvavkevGdetfrlckeylddivlvdtdev 257
                                                    i++igve+e sa+l++al aGerv l +vG fadGvav ++G++ f++c+  +d++++v  de+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758 206 IRIIGVESEHSACLQAALRAGERVVLPTVGTFADGVAVAQIGAYGFEICRFCVDEVLTVSNDEL 269
                                                    **************************************************************** PP

                                      TIGR01124 258 caaikdvfedtravlepaGalalaGlkkyvakkgiedktlvailsGanlnfdrlryvserael 320
                                                    caaik++++dtr+++ep+Gala+aG+k+yva+ g++++tlvai sGan+nfd+lr+v+era l
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4758 270 CAAIKNIYDDTRSITEPSGALAVAGIKQYVARTGARGQTLVAIDSGANINFDSLRHVAERAAL 332
                                                    ************************************************************976 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (499 nodes)
Target sequences:                          1  (337 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.83
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis