Alignments for a candidate for dapA in Pseudomonas fluorescens FW300-N1B4

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapA in Pseudomonas fluorescens FW300-N1B4

Align 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7) (characterized)
to candidate Pf1N1B4_3332 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7)

Query= BRENDA::Q9I4W3
         (292 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332
          Length = 292

 Score =  493 bits (1269), Expect = e-144
 Identities = 248/291 (85%), Positives = 266/291 (91%)

Query: 1   MIAGSMVALVTPFDAQGRLDWDSLAKLVDFHLQEGTNAIVAVGTTGESATLDVEEHIQVI 60
           MIAGSMVALVTP DAQGRLDWDSL+KLVDFHLQEGTNAIVAVGTTGESATLDV EHI+VI
Sbjct: 1   MIAGSMVALVTPMDAQGRLDWDSLSKLVDFHLQEGTNAIVAVGTTGESATLDVNEHIEVI 60

Query: 61  RRVVDQVKGRIPVIAGTGANSTREAVALTEAAKSGGADACLLVTPYYNKPTQEGMYQHFR 120
           RRVV QV GRIPVIAGTGANSTREA+ LT  AK+ GADACLLVTPYYNKPTQEG+YQHF+
Sbjct: 61  RRVVAQVAGRIPVIAGTGANSTREAIELTTNAKNAGADACLLVTPYYNKPTQEGLYQHFK 120

Query: 121 HIAEAVAIPQILYNVPGRTSCDMLPETVERLSKVPNIIGIKEATGDLQRAKEVIERVGKD 180
            IAEAV IPQILYNVPGRT+CDM  ETV RLS V NIIGIKEATGDLQRAK+++  V  D
Sbjct: 121 AIAEAVDIPQILYNVPGRTACDMQAETVIRLSTVKNIIGIKEATGDLQRAKDILAGVSSD 180

Query: 181 FLVYSGDDATAVELMLLGGKGNISVTANVAPRAMSDLCAAAMRGDAAAARAINDRLMPLH 240
           FLVYSGDDATAVELMLLGGKGNISVTANVAPRAMS+LCAAAM GDA  ARAI+++LMPL+
Sbjct: 181 FLVYSGDDATAVELMLLGGKGNISVTANVAPRAMSELCAAAMAGDAVKARAIHEKLMPLN 240

Query: 241 KALFIESNPIPVKWALHEMGLIPEGIRLPLTWLSPRCHEPLRQAMRQTGVL 291
           K LFIESNPIPVKWALHEMGL+P+GIRLPLTWLS  CHEPLRQAMRQ+GVL
Sbjct: 241 KTLFIESNPIPVKWALHEMGLMPDGIRLPLTWLSAACHEPLRQAMRQSGVL 291


Lambda     K      H
   0.319    0.134    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 369
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 292
Length of database: 292
Length adjustment: 26
Effective length of query: 266
Effective length of database: 266
Effective search space:    70756
Effective search space used:    70756
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate Pf1N1B4_3332 (4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))
to HMM TIGR00674 (dapA: 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00674.hmm
# target sequence database:        /tmp/gapView.23287.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00674  [M=286]
Accession:   TIGR00674
Description: dapA: 4-hydroxy-tetrahydrodipicolinate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                      Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                      -----------
   1.7e-118  380.6   0.1   1.9e-118  380.5   0.1    1.0  1  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332  4-hydroxy-tetrahydrodipicolinate


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332  4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  380.5   0.1  1.9e-118  1.9e-118       2     284 ..       5     286 ..       4     288 .. 0.98

  Alignments for each domain:
  == domain 1  score: 380.5 bits;  conditional E-value: 1.9e-118
                                      TIGR00674   2 vltAliTPfkedgsvdfaalekliesqiekgvdaivvvGtTGEsatLsleEkkkvievavelvk 65 
                                                    +++Al+TP++++g +d ++l+kl+++++++g++aiv+vGtTGEsatL ++E+++vi+ +v  v+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332   5 SMVALVTPMDAQGRLDWDSLSKLVDFHLQEGTNAIVAVGTTGESATLDVNEHIEVIRRVVAQVA 68 
                                                    799************************************************************* PP

                                      TIGR00674  66 nrvpviaGtgsnateeaieltkeaeklgvdgvlvvtPyYnkPtqeGlykhfkaiaeevelPiil 129
                                                    +r+pviaGtg+n+t+eaielt+ a+++g+d++l+vtPyYnkPtqeGly+hfkaiae+v++P+il
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332  69 GRIPVIAGTGANSTREAIELTTNAKNAGADACLLVTPYYNKPTQEGLYQHFKAIAEAVDIPQIL 132
                                                    **************************************************************** PP

                                      TIGR00674 130 YnvPsRtgvslepetvkrLaeeveivaiKeasgdlervseikaeakedfkvlsGdDaltleila 193
                                                    YnvP+Rt+++++ etv+rL++ ++i++iKea+gdl+r++ i a ++ df v+sGdDa++ e+++
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332 133 YNVPGRTACDMQAETVIRLSTVKNIIGIKEATGDLQRAKDILAGVSSDFLVYSGDDATAVELML 196
                                                    **************************************************************** PP

                                      TIGR00674 194 lGakGviSVasnvapkelkemvkaalegdteeareihqkllklfkalfietNPipvKtalallg 257
                                                    lG+kG iSV++nvap+ ++e+++aa++gd  +ar ih+kl++l k+lfie+NPipvK+al+ +g
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332 197 LGGKGNISVTANVAPRAMSELCAAAMAGDAVKARAIHEKLMPLNKTLFIESNPIPVKWALHEMG 260
                                                    **************************************************************** PP

                                      TIGR00674 258 liekdelRlPLtelseekkeklkevlk 284
                                                    l+    +RlPLt ls   +e l+++++
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3332 261 LMPD-GIRLPLTWLSAACHEPLRQAMR 286
                                                    **99.9*********999999888776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (286 nodes)
Target sequences:                          1  (292 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.53
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis