GapMind for Amino acid biosynthesis

 

Alignments for a candidate for trpE in Pseudomonas fluorescens FW300-N1B4

Align Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 (characterized)
to candidate Pf1N1B4_2545 Anthranilate synthase, aminase component (EC 4.1.3.27)

Query= SwissProt::P20579
         (493 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2545
          Length = 493

 Score =  879 bits (2271), Expect = 0.0
 Identities = 439/490 (89%), Positives = 457/490 (93%)

Query: 1   MNREEFLRLAAVGYNRIPLACETLADFDTPLSIYLKLADQPNSYLLESVQGGEKWGRYSM 60
           M REEFLRLAA GYNRIPLACETLADFDTPLSIYLKLAD+PNSYLLESVQGGEKWGRYS+
Sbjct: 1   MIREEFLRLAAAGYNRIPLACETLADFDTPLSIYLKLADEPNSYLLESVQGGEKWGRYSI 60

Query: 61  IGLPSRTVMRVHGYHVSILHDGVEVESHDVEDPLAFVESFKDRYKVADIPGLPRFNGGLV 120
           IGLP RTV+RVH +HVS+ HDGVE+ESHDVEDPLAFVE+FK RY V  I GLPRFNGGLV
Sbjct: 61  IGLPCRTVLRVHDHHVSVTHDGVEIESHDVEDPLAFVEAFKARYNVPTIAGLPRFNGGLV 120

Query: 121 GYFGYDCVRYVEKRLGVSPNPDPLGVPDILLMVSDAVVVFDNLAGKMHAIVLVDPAEEQA 180
           GYFGYDCVRYVEKRLG  PNPDPLGVPDILLMVSDAVVVFDNLAGKMHAIVL DP++E A
Sbjct: 121 GYFGYDCVRYVEKRLGKCPNPDPLGVPDILLMVSDAVVVFDNLAGKMHAIVLADPSQEDA 180

Query: 181 FEQGQARLQGLLETLRQPITPRRGLDLSGPQAAEPEFRSSYTREDYENAVGRIKEYILAG 240
           FEQGQARLQ LLE LRQPITPRRGLD S  Q+A+P FRSS+T+ DYE AV  IKEYILAG
Sbjct: 181 FEQGQARLQALLEQLRQPITPRRGLDFSKQQSADPVFRSSFTQNDYEKAVDTIKEYILAG 240

Query: 241 DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNL 300
           DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNL
Sbjct: 241 DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNL 300

Query: 301 VTVRPIAGTRPRGATEEADRALEDDLLSDDKEIAEHLMLIDLGRNDVGRVSSTGSVRLTE 360
           +TVRPIAGTRPRGA EEAD ALE DLLSDDKEIAEHLMLIDLGRND GRVS  GSV+LTE
Sbjct: 301 ITVRPIAGTRPRGANEEADVALEKDLLSDDKEIAEHLMLIDLGRNDTGRVSEIGSVKLTE 360

Query: 361 KMVIERYSNVMHIVSNVAGQLREGLTAMDALRAILPAGTLSGAPKIRAMEIIDELEPVKR 420
           KMVIERYSNVMHIVSNV GQL+ GLTAMDALRAILPAGTLSGAPKIRAMEIIDELEPVKR
Sbjct: 361 KMVIERYSNVMHIVSNVTGQLKAGLTAMDALRAILPAGTLSGAPKIRAMEIIDELEPVKR 420

Query: 421 GVYGGAVGYFAWNGNMDTAIAIRTAVINDGELHVQAGGGIVADSVPALEWEETINKRRAM 480
           GVYGGAVGYFAWNGNMDTAIAIRTAVI +GELHVQAGGGIVADSVPALEWEET+NKRRAM
Sbjct: 421 GVYGGAVGYFAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETLNKRRAM 480

Query: 481 FRAVALAEQT 490
           FRAVALAEQT
Sbjct: 481 FRAVALAEQT 490


Lambda     K      H
   0.320    0.138    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 903
Number of extensions: 20
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 493
Length of database: 493
Length adjustment: 34
Effective length of query: 459
Effective length of database: 459
Effective search space:   210681
Effective search space used:   210681
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

Align candidate Pf1N1B4_2545 (Anthranilate synthase, aminase component (EC 4.1.3.27))
to HMM TIGR00564 (trpE: anthranilate synthase component I (EC 4.1.3.27))

Software error:

../bin/blast/fastacmd -i /tmp/list.14030.in -d ../tmp/orgsFit/orgs.faa -p T > /tmp/gapView.14030.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

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