Align Acetylornithine deacetylase; AO; Acetylornithinase; N-acetylornithinase; NAO; EC 3.5.1.16 (characterized)
to candidate AO353_08690 AO353_08690 acetylornithine deacetylase
Query= SwissProt::P23908 (383 letters) >FitnessBrowser__pseudo3_N2E3:AO353_08690 Length = 382 Score = 362 bits (930), Expect = e-105 Identities = 193/379 (50%), Positives = 243/379 (64%), Gaps = 5/379 (1%) Query: 5 LPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFKDLGFNVEVQPV-PGTRNKFN 63 LP + + ALIA PS+S T+ +LDQSN +I LLA W DLGF+ ++Q V PG KFN Sbjct: 3 LPSMKDQFAALIAAPSVSCTQPSLDQSNRAVIDLLATWLGDLGFSCDIQQVSPG---KFN 59 Query: 64 MLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHDGKLYGLGTADMKGFFAFILDA 123 +LAS G G GGL+LAGH+DTVPFD W DP LTE DG+ GLG+ DMKGFFA ++A Sbjct: 60 LLASFGSGPGGLVLAGHSDTVPFDGALWQTDPLKLTEVDGRWVGLGSCDMKGFFALAIEA 119 Query: 124 LRDVDVTKLKKPLYILATADEETSMAGARYFAETTALRPDCAIIGEPTSLQPVRAHKGHI 183 + + K+PL ILAT DEE+SMAGAR A A+IGEPT L+P+R HKG + Sbjct: 120 VLPLLDKPFKQPLMILATCDEESSMAGARALAAAGRPLGRVAVIGEPTGLKPIRMHKGVM 179 Query: 184 SNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQLRDNLKERYHYEAFTVPYPTLNLGH 243 I I GQSGHSSDP G +A+E MHDA+G + LR + +H F+VP PTLN G Sbjct: 180 MERIDILGQSGHSSDPRLGHSALEAMHDAMGELRGLRLQWQREFHNPQFSVPQPTLNFGC 239 Query: 244 IHGGDASNRICACCELHMDIRPLPGMTLNELNGLLNDALAPVSERWPGRLTVDELHPPIP 303 IHGGD NRIC C L D+RPLPGM N L + L PV+E ++ L P +P Sbjct: 240 IHGGDNPNRICGQCSLEFDLRPLPGMDPNALRAAIRQKLEPVAELHQVKIDYAPLFPEVP 299 Query: 304 GYECPPNHQLVEVVEKLLGAKTEVVNYCTEAPFIQTL-CPTLVLGPGSINQAHQPDEYLE 362 +E + +LV+V E+L G E V + TEAP++Q L C TLVLGPG I AHQP EYLE Sbjct: 300 PFEQAADSELVQVAERLTGHLAEAVAFGTEAPYLQRLGCETLVLGPGDIACAHQPGEYLE 359 Query: 363 TRFIKPTRELITQVIHHFC 381 ++PT L+ Q+I H+C Sbjct: 360 MSRLQPTVHLLRQLIEHYC 378 Lambda K H 0.320 0.139 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 461 Number of extensions: 18 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 383 Length of database: 382 Length adjustment: 30 Effective length of query: 353 Effective length of database: 352 Effective search space: 124256 Effective search space used: 124256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate AO353_08690 AO353_08690 (acetylornithine deacetylase)
to HMM TIGR01892 (argE: acetylornithine deacetylase (ArgE) (EC 3.5.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01892.hmm # target sequence database: /tmp/gapView.19682.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01892 [M=365] Accession: TIGR01892 Description: AcOrn-deacetyl: acetylornithine deacetylase (ArgE) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-121 391.8 0.0 1.7e-121 391.7 0.0 1.0 1 lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 AO353_08690 acetylornithine deac Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 AO353_08690 acetylornithine deacetylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 391.7 0.0 1.7e-121 1.7e-121 3 365 .] 10 374 .. 8 374 .. 0.97 Alignments for each domain: == domain 1 score: 391.7 bits; conditional E-value: 1.7e-121 TIGR01892 3 lakLvafdsvsar......snvdlieyvedyleelgvavevlpvadgaeksnllaviGpkegagg 61 +a+L+a +svs + sn ++i++++ +l +lg++ + ++v g k+nlla+ G +g gg lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 10 FAALIAAPSVSCTqpsldqSNRAVIDLLATWLGDLGFSCDIQQVSPG--KFNLLASFG--SGPGG 70 789*********9999******************************9..*********..999** PP TIGR01892 62 lvlsGhtDvvPvdeaaWtsDpfrLtekdgrLYgrGtaDmkGFlalvLaavpdlaaakLkkPlhlv 126 lvl+Gh+D+vP+d+a W +Dp++Lte dgr g+G++DmkGF+al+++av l ++ k+Pl+++ lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 71 LVLAGHSDTVPFDGALWQTDPLKLTEVDGRWVGLGSCDMKGFFALAIEAVLPLLDKPFKQPLMIL 135 ***************************************************************** PP TIGR01892 127 lsaDeevglaGakkliealarrpalaivGePtslvavRahkGkasakvtvrGreghssrpdrGvs 191 ++ Dee ++aGa+ l++a+ + ++a++GePt+l+++R hkG + ++ + G++ghss+p G s lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 136 ATCDEESSMAGARALAAAGRPLGRVAVIGEPTGLKPIRMHKGVMMERIDILGQSGHSSDPRLGHS 200 ***************************************************************** PP TIGR01892 192 aielaakllarlvaladklkredleeaFtppyatlniGtvkGGkavniiaaaCelvlelRpipGm 256 a e +++++++l +l+ + +re ++ F +p +tln G ++GG+ n+i+++C l+++lRp+pGm lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 201 ALEAMHDAMGELRGLRLQWQREFHNPQFSVPQPTLNFGCIHGGDNPNRICGQCSLEFDLRPLPGM 265 ***************************************************************** PP TIGR01892 257 dpeellallekiaeevkekapgfevkveelsatpaleleedaelvalleklaGaaaevvsygtea 321 dp++l a +++ +e+v e + + + + p +e +d+elv+++e+l+G+ ae+v++gtea lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 266 DPNALRAAIRQKLEPVAELHQVKIDYAPLFPEVPPFEQAADSELVQVAERLTGHLAEAVAFGTEA 330 ***************9665555555566788999******************************* PP TIGR01892 322 gllqelGieavvlGPGdidqahqpdeYveieelkrcrallerlv 365 ++lq+lG+e++vlGPGdi ahqp+eY+e++ l+++ ll++l+ lcl|FitnessBrowser__pseudo3_N2E3:AO353_08690 331 PYLQRLGCETLVLGPGDIACAHQPGEYLEMSRLQPTVHLLRQLI 374 ****************************************9986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (365 nodes) Target sequences: 1 (382 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.58 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory