Alignments for a candidate for cysK in Pseudomonas fluorescens FW300-N2E3

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Pseudomonas fluorescens FW300-N2E3

Align Homocysteine/cysteine synthase; O-acetylserine/O-acetylhomoserine sulfhydrylase; OAS-OAH SHLase; OAS-OAH sulfhydrylase; EC 2.5.1.47; EC 2.5.1.49 (characterized)
to candidate AO353_23295 AO353_23295 cystathionine beta-lyase

Query= SwissProt::P06106
         (444 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_23295
          Length = 392

 Score =  208 bits (529), Expect = 3e-58
 Identities = 152/439 (34%), Positives = 224/439 (51%), Gaps = 58/439 (13%)

Query: 2   PSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQLFGLEVPGYVYSRFQN 61
           P  F T  +HAGQ    D    +   PIYA ++Y+ ++        G+   G+ Y R  N
Sbjct: 10  PRAFATRVIHAGQTP--DPTTGALMPPIYANSTYLQQSP-------GVH-KGFDYGRSHN 59

Query: 62  PTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNIVSTSYLYGGTYNQF-KI 120
           PT   LE  +A LEGG  A A +SG AA +  ++ L  TG +IVS + LYGGT+  F K+
Sbjct: 60  PTRFALERCVADLEGGTQAFAFASGLAAISTVLE-LLDTGSHIVSGNDLYGGTFRLFDKV 118

Query: 121 SFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNVPDFEKIVAIAHKHGIPV 180
             +  G    FV+  +   FE    + T+ V++ET  NP   + D   +     + GI  
Sbjct: 119 RRRSAGHRFSFVDLTDLTAFEAALQDDTRMVWVETPSNPLLRITDLAAVARTCRERGIIC 178

Query: 181 VVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGIIVDSGKFPWKDYPEKFPQF 240
           V DNTF A     +P++ G DIV HS TK++ GH   IGGI V                 
Sbjct: 179 VADNTF-ASPRIQRPLELGFDIVLHSTTKYLNGHSDVIGGIAV----------------V 221

Query: 241 SQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFASFLLLQGVETLSLRAERHG 300
            Q AE        E  G L   V          G +  PF +FL L+GV+TL+LR ERH 
Sbjct: 222 GQNAE------LRERLGFLQNAV----------GAIAGPFDAFLTLRGVKTLALRMERHC 265

Query: 301 ENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNGFGGVLSFGVKDLPNADKETD 360
            NAL+LA+WL   P V+ V YPGL SH  HE A++ + +GFGG++S         D +TD
Sbjct: 266 SNALELARWLSHQPQVARVYYPGLPSHPQHELAQRQM-HGFGGMISL--------DLDTD 316

Query: 361 PFKLSGA-QVVDNLKLASNLANVGDAKTLVIAPYFTTHKQLNDKEKLASGVTKDLIRVSV 419
              L+GA + ++++++ +   ++G  ++L+  P   TH  +  + +   G+   LIR+SV
Sbjct: 317 ---LAGAKRFLESVQIFALAESLGGVESLIEHPAIMTHASIPAETRAELGIGDALIRLSV 373

Query: 420 GIEFIDDIIADFQQSFETV 438
           GIE ++D+ AD  Q+   +
Sbjct: 374 GIEDVEDLRADLAQALAQI 392


Lambda     K      H
   0.317    0.136    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 430
Number of extensions: 30
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 444
Length of database: 392
Length adjustment: 32
Effective length of query: 412
Effective length of database: 360
Effective search space:   148320
Effective search space used:   148320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis