Align Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 (characterized)
to candidate AO353_12170 AO353_12170 glutamine synthetase
Query= SwissProt::Q3V5W6 (468 letters) >FitnessBrowser__pseudo3_N2E3:AO353_12170 Length = 468 Score = 915 bits (2364), Expect = 0.0 Identities = 442/468 (94%), Positives = 456/468 (97%) Query: 1 MSKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGI 60 MSKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDAL++ FFE GKMFDGSSIAGWKGI Sbjct: 1 MSKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALDEAFFEEGKMFDGSSIAGWKGI 60 Query: 61 EASDMILLPDDDTAVLDPFTEDATLILVCDIIEPSTMQGYDRDPRAIAHRAEEYLKTTGI 120 EASDMIL+PDD TAVLDPFTE+ TLILVCD+IEPSTMQGYDRDPRAIA RAEEYLK+TGI Sbjct: 61 EASDMILMPDDTTAVLDPFTEEPTLILVCDVIEPSTMQGYDRDPRAIAKRAEEYLKSTGI 120 Query: 121 GDTVFAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSDQDIEGGNKGHRPGVKGGYF 180 GDTVF GPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSDQD+EGGNKGHRPGVKGGYF Sbjct: 121 GDTVFVGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSDQDVEGGNKGHRPGVKGGYF 180 Query: 181 PVPPFDHDHEIRTAMCNALEEMGQTVEVHHHEVATAGQNEIGVKFNTLVKKADEVQTLKY 240 PVPPFDHDHEIRT+MCNA+EEMG +EVHHHEVATAGQNEIGVKFNTLV KADEVQTLKY Sbjct: 181 PVPPFDHDHEIRTSMCNAMEEMGLVIEVHHHEVATAGQNEIGVKFNTLVAKADEVQTLKY 240 Query: 241 VVHNVADAYGRTATFMPKPLYGDNGSGMHVHMSIAKDGKNTFAGEGYAGLSETALYFIGG 300 VHNVADAYGRTATFMPKPLYGDNGSGMHVH+SIAKDGKNTFAGEGYAGLS+TALYFIGG Sbjct: 241 CVHNVADAYGRTATFMPKPLYGDNGSGMHVHLSIAKDGKNTFAGEGYAGLSDTALYFIGG 300 Query: 301 IIKHGKALNGFTNPATNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVNSPRGRRIEARF 360 IIKHGKALNGFTNP+TNSYKRLVPGFEAPVMLAYSARNRSASIRIPYV+SPR RRIEARF Sbjct: 301 IIKHGKALNGFTNPSTNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVSSPRARRIEARF 360 Query: 361 PDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEE 420 PDPAANPYL FAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEE Sbjct: 361 PDPAANPYLGFAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEE 420 Query: 421 LDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC 468 LDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC Sbjct: 421 LDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC 468 Lambda K H 0.318 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 944 Number of extensions: 39 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 468 Length of database: 468 Length adjustment: 33 Effective length of query: 435 Effective length of database: 435 Effective search space: 189225 Effective search space used: 189225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate AO353_12170 AO353_12170 (glutamine synthetase)
to HMM TIGR00653 (glnA: glutamine synthetase, type I (EC 6.3.1.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00653.hmm # target sequence database: /tmp/gapView.10683.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00653 [M=462] Accession: TIGR00653 Description: GlnA: glutamine synthetase, type I Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-204 664.8 0.0 3.5e-204 664.7 0.0 1.0 1 lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 AO353_12170 glutamine synthetase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 AO353_12170 glutamine synthetase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 664.7 0.0 3.5e-204 3.5e-204 4 462 .] 5 467 .. 2 467 .. 0.98 Alignments for each domain: == domain 1 score: 664.7 bits; conditional E-value: 3.5e-204 TIGR00653 4 lkllkeenvkfvdlrfvDikGklkkveipvse.leeealeegiaFDgssveGfksieesDlllkp 67 ++l+k+++vk +dlrf+D+kG+ ++v++p+++ l+e ++eeg++FDgss+ G+k+ie sD++l+p lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 5 VQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDaLDEAFFEEGKMFDGSSIAGWKGIEASDMILMP 69 6899*************************985267999*************************** PP TIGR00653 68 dpetlvivPfraekvlrvicdvyepvtkepyerdpRsiakraeeelk.tklgdevyfGpEaEFfl 131 d t+v +Pf++e++l+++cdv ep t++ y+rdpR iakraee+lk t++gd+v++GpE+EFf+ lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 70 DDTTAVLDPFTEEPTLILVCDVIEPSTMQGYDRDPRAIAKRAEEYLKsTGIGDTVFVGPEPEFFI 134 ***************************************************************** PP TIGR00653 132 fdkvefkeasnssflevdseegewn..reveegnkgykikkkggYfdvepvDkakdirrelvlal 194 fd+v+fk+ + s++++ se+g+w ++ve+gnkg+++ kggYf+v+p D+ ++ir+++++a+ lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 135 FDEVKFKSDISGSMFKIYSEQGSWMsdQDVEGGNKGHRPGVKGGYFPVPPFDHDHEIRTSMCNAM 199 ************************988899999******************************** PP TIGR00653 195 eelglevevsHHEvata.qaEidikfdklvkaaDeivlyKyvvknvakkhGktatFmpKplfgdn 258 ee+gl +ev+HHEvata q+Ei++kf++lv +aDe++++Ky v+nva+ +G+tatFmpKpl+gdn lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 200 EEMGLVIEVHHHEVATAgQNEIGVKFNTLVAKADEVQTLKYCVHNVADAYGRTATFMPKPLYGDN 264 ***************************************************************** PP TIGR00653 259 gsGmHvhlslwkdgenlfageegyagLsetalyyigGilkHakalaAltnptvnsYkRLvpGyEA 323 gsGmHvhls+ kdg+n fage yagLs+taly+igGi+kH+kal+ +tnp++nsYkRLvpG+EA lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 265 GSGMHVHLSIAKDGKNTFAGEG-YAGLSDTALYFIGGIIKHGKALNGFTNPSTNSYKRLVPGFEA 328 *********************9.****************************************** PP TIGR00653 324 PvylaysaknRsaliRiPasanpkakRiEvRspDpsanpYLafaallmAgldGiknkidpgepld 388 Pv+laysa+nRsa+iRiP++++p+a+RiE R+pDp+anpYL faallmAgldGi+nki+pg++ d lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 329 PVMLAYSARNRSASIRIPYVSSPRARRIEARFPDPAANPYLGFAALLMAGLDGIQNKIHPGDAAD 393 ***************************************************************** PP TIGR00653 389 knlyelseeelkelGieqlpesLeealdelesdk..evlkevlgeelieafielkrkEveelrlk 451 knly+l++ee+ke i+q+ sL+eal+el++ + +++++v+++++i+a+i+lk +E ++r+ lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 394 KNLYDLPPEEAKE--IPQVCGSLKEALEELDKGRafLTKGGVFSDDFIDAYIALKSEEEIKVRTF 456 *************..******************99999*************************** PP TIGR00653 452 vhpvElekyld 462 vhp+E e y++ lcl|FitnessBrowser__pseudo3_N2E3:AO353_12170 457 VHPLEYELYYS 467 *********95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (462 nodes) Target sequences: 1 (468 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 7.54 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory