Alignments for a candidate for glyA in Pseudomonas fluorescens FW300-N2E3

GapMind for Amino acid biosynthesis

Alignments for a candidate for glyA in Pseudomonas fluorescens FW300-N2E3

Align glycine hydroxymethyltransferase (EC 2.1.2.1) (characterized)
to candidate AO353_10495 AO353_10495 serine hydroxymethyltransferase

Query= BRENDA::R9YJZ9
         (417 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_10495
          Length = 421

 Score =  588 bits (1517), Expect = e-173
 Identities = 287/420 (68%), Positives = 334/420 (79%), Gaps = 4/420 (0%)

Query: 1   MLKKAMNIADYDPELFKAIEDETRRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP 60
           M    + +  +D EL +AI +E  RQE+H+ELIASENY SP VM+ Q + LTNKYAEGYP
Sbjct: 1   MYDSTLTLKAFDAELCEAIHNEEHRQEDHVELIASENYASPLVMKIQSTVLTNKYAEGYP 60

Query: 61  GKRYYGGCEYVDVVETLAIERAKQLFGATYANVQPHSGSQANSAVYMALLQPGDTVLGMN 120
           GKRYY GCEYVDV E LA+ER K LF   YANVQPH+G+QAN+AV++AL+ PGDTV+GMN
Sbjct: 61  GKRYYSGCEYVDVAERLAVERVKTLFNCDYANVQPHAGAQANAAVFLALINPGDTVMGMN 120

Query: 121 LAHGGHLTHGSPVNFSGKLYNIIPYGID-ESGKIDYSELETLALEHKPKMIIGGFSAYSG 179
           LA GGHLTHG+P NFSG+ Y I+PYG+D ++G +DY E+E +ALE +PKM+IGGFSAYS 
Sbjct: 121 LAQGGHLTHGNPSNFSGRHYKIVPYGLDPKTGFLDYDEMERIALETRPKMLIGGFSAYSR 180

Query: 180 IVDWAKLREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVI 239
             DWA++R IADK+GA  +VDMAHVAGL+AAG YP+P+PHAHVVTSTTHKTL GPRGG+I
Sbjct: 181 YKDWARMRAIADKVGAIFWVDMAHVAGLVAAGEYPDPLPHAHVVTSTTHKTLRGPRGGLI 240

Query: 240 LSAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKTYQQQVVNNAKAMV 299
           LS   DE  YKKL+SAVFPG QGGPLMH IA KA+AFKEAL PEFKTYQ QVV NA+AM 
Sbjct: 241 LSKGQDEAFYKKLDSAVFPGVQGGPLMHQIAAKAIAFKEALAPEFKTYQTQVVINARAMA 300

Query: 300 EVFLERGYKIVSGGTDNHLMLVDLIGRELTGKEADAALGRANITVNKNSVPNDPRSPFVT 359
            V   RGYKIVSGGTDNH+ML+DL  R  TGKEADAAL  A IT NKNSVPNDPRSPFVT
Sbjct: 301 AVLQRRGYKIVSGGTDNHMMLIDLSDRPYTGKEADAALSNAYITANKNSVPNDPRSPFVT 360

Query: 360 SGVRIGTPAITRRGFKEAEARELTGWICDVLD---NAHDDAVIERVKSQVLELCARFPVY 416
           SG+RIGTPAIT RGF   E  +L GW+CDVLD   N H + V   V+ QV+ELC R PVY
Sbjct: 361 SGLRIGTPAITTRGFGVFECEQLAGWLCDVLDALENGHSETVGHHVREQVVELCRRHPVY 420


Lambda     K      H
   0.316    0.135    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 623
Number of extensions: 29
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 421
Length adjustment: 32
Effective length of query: 385
Effective length of database: 389
Effective search space:   149765
Effective search space used:   149765
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis