GapMind for Amino acid biosynthesis

 

Protein AO356_21400 in Pseudomonas fluorescens FW300-N2C3

Annotation: FitnessBrowser__pseudo5_N2C3_1:AO356_21400

Length: 472 amino acids

Source: pseudo5_N2C3_1 in FitnessBrowser

Candidate for 3 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine biosynthesis leuC hi 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized) 64% 100% 596.3
L-isoleucine biosynthesis leuC hi leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33) (TIGR00170) 100% 775.7
L-leucine biosynthesis leuC hi 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized) 64% 100% 596.3
L-leucine biosynthesis leuC hi leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33) (TIGR00170) 100% 775.7
L-lysine biosynthesis lysT lo methanogen homoaconitase (EC 4.2.1.114) (characterized) 34% 98% 224.6 alpha isopropylmalate isomerase (Eurofung) 64% 605.1

Sequence Analysis Tools

View AO356_21400 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAGRKPWRIDANIA
TPDHNVPTTPERKGGIEAIADQVSRLQVQTLDDNCDEYGIVEFKMNDVRQGIVHVIGPEQ
GATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKNMLVRVEGKLP
FGVTAKDIVLAVIGKIGTAGGNGHAIEFAGSAIRDLSVEGRMTICNMSIEAGARVGLVAA
DEKTVEYVKGRPFSPKGAEWDMAVEAWKDLVSDADAKFDTVVELDATQIKPQVSWGTSPE
MVLAVDQNVPDPAKEMDLVKRDSIVRALKYMGLSANQAITDIQLDRVFIGSCTNSRIEDL
RAAAVIAKGRKVASTIKQAIVVPGSGLVKAQAEAEGLDKIFLEAGFEWREPGCSMCLAMN
PDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFVDVRELI

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory