GapMind for Amino acid biosynthesis

 

Aligments for a candidate for argA in Pseudomonas fluorescens FW300-N2C3

Align Arginine biosynthesis bifunctional protein ArgJ; EC 2.3.1.35; EC 2.3.1.1 (characterized)
to candidate AO356_12410 AO356_12410 ornithine acetyltransferase

Query= SwissProt::Q07908
         (410 letters)



>lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_12410 AO356_12410
           ornithine acetyltransferase
          Length = 385

 Score =  275 bits (703), Expect = 2e-78
 Identities = 167/401 (41%), Positives = 232/401 (57%), Gaps = 26/401 (6%)

Query: 17  TVVTPEGFQAAGVNAGLRYSKNDLGVILCDVPASAAAVYTQSHFQAAPLKVTQASLAVEQ 76
           T + P+GF++   N G++ S++D   ++ DVPAS +AV+TQS F A P       +A   
Sbjct: 4   THIQPKGFRSIIANLGIKDSRDDFMAVISDVPASISAVFTQSRF-AGPSVTLSREVAQRD 62

Query: 77  KLQAVIVNRPCANACTGAQGLKDAYEMRELCAKQFGLALHHVAVASTGVIGEYLPMEKIR 136
             Q V+V    AN  TG +GL +A E+R   A+   +    + +ASTGVIG   PMEKIR
Sbjct: 63  TAQGVVVIARNANVATGPEGLANAQEVRAGLARAVDVDPQALVIASTGVIGRQYPMEKIR 122

Query: 137 AGIKQLVPGVTMADAEAFQTAILTTDTVMKRACYQTTIDGKTVTVGGAAKGSGMIHPNMA 196
             +   VP +  AD +    AI+TTDT  K A  Q      + ++ G AKG GMI PNMA
Sbjct: 123 TFLGN-VPPLQPADFQRCAAAIMTTDTHTKYAARQVG----SASLVGIAKGVGMIEPNMA 177

Query: 197 TMLAFITTDANVSSPVLHAALRSITDVSFNQITVDGDTSTNDMVVVMASGLAGNDELTPD 256
           T+L F  TDA ++  VL A  R + D +FN +++D DTST+D   ++A+GLAG  ++   
Sbjct: 178 TLLTFFFTDARIAPDVLDALFRRVIDKTFNALSIDTDTSTSDSAAILANGLAGEVDMV-- 235

Query: 257 HPDWENFYEALRKTCEDLAKQIAKDGEGATKLIEVRVRGAKTDEEAKKIAKQIVGSNLVK 316
                 F +AL      L + IA DGEGA+K +EV V GA+ D +AK++AK IV S LVK
Sbjct: 236 -----EFEQALYDIALGLVRMIASDGEGASKALEVHVTGARDDAQAKRVAKAIVNSPLVK 290

Query: 317 TAVYGADANWGRIIGAIGYSDAE--VNPDNVDVAIGPMVMLKGSEPQPFSEEEAA----- 369
           TAV+GAD NWGR+  AIG  +AE  ++PD V +  G       +E  P   +EA      
Sbjct: 291 TAVHGADPNWGRVAMAIGKCEAEQDIDPDKVRIVFGE------TEAYPQQLDEAGLLAVK 344

Query: 370 AYLQQETVVIEVDLHIGDGVGVAWGCDLTYDYVKINASYRT 410
            YLQ + V+I VDL+I  G    +GCDL+  Y++INA Y T
Sbjct: 345 GYLQGKEVLISVDLNIAQGQFTVYGCDLSEGYIRINADYTT 385


Lambda     K      H
   0.316    0.130    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 367
Number of extensions: 16
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 410
Length of database: 385
Length adjustment: 31
Effective length of query: 379
Effective length of database: 354
Effective search space:   134166
Effective search space used:   134166
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory