Align Threonine synthase; TS; EC 4.2.3.1 (uncharacterized)
to candidate AO356_27150 AO356_27150 threonine synthase
Query= curated2:P29363 (469 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_27150 Length = 467 Score = 400 bits (1027), Expect = e-116 Identities = 201/465 (43%), Positives = 292/465 (62%), Gaps = 16/465 (3%) Query: 1 MRYISTRGQAPALNFEDVLLAGLASDGGLYVPENLPRFTLEEIASWVGLPYHELAFRVMR 60 M+Y+STR F +VL+ A DGGLYVP +PRF EE++ LP+HELA V+ Sbjct: 1 MKYVSTRTPGRTFEFSEVLMGSYAPDGGLYVPRKVPRFKKEELSKLEWLPFHELARHVIE 60 Query: 61 PFVAGSIADADFKKILEETYGVFAHDAVAPLRQLNGNEWVLELFHGPTLAFKDFALQLLG 120 PF+ G + ++LE+T+ F ++AVAPL Q NEW+LELFHGP+ A++DF LQL+ Sbjct: 61 PFLGGWVEQDQLSRMLEDTFDCFTNNAVAPLYQTASNEWLLELFHGPSGAYQDFGLQLMS 120 Query: 121 RLLDHVLAKRGERVVIMGATSGDTGSAAIEGCRRCDNVDIFIMHPHNRVSEVQRRQMTTI 180 R ++H L K G++ +I+G T GDTG+AAI+ V + ++HP ++ RR++ ++ Sbjct: 121 RFINHDLLKSGKKALIVGCTGGDTGAAAIKAFSGMTGVTLLVLHPSKELTAEDRRRLLSV 180 Query: 181 LGDNIHNIAIEGNFDDCQEMVKASFADQGFLKGTRLVAVNSINWARIMAQIVYYFHAALQ 240 N+ NI I G++ DC + + F Q LV+ NS++W R++A + +YF++ALQ Sbjct: 181 EEGNVINIEIAGDYSDCHRLCE-QFLKQNNDAQQHLVSFNSVHWVRVLAHLSFYFYSALQ 239 Query: 241 LGAPHRSVAFSVPTGNFGDIFAGYLARNMGLPVSQLIVATNRNDILHRFMSGNRYDKDTL 300 LGAP R VAFSVPTGNFG ++AGY+A+ MGLP+ QLIVATN N LH F+ N Y ++ + Sbjct: 240 LGAPKRQVAFSVPTGNFGALYAGYIAKQMGLPIRQLIVATNANAGLHHFLQSNLYSRNEI 299 Query: 301 HPSLSPSMDIMVSSNFERLLFDLHGRNGKAVAELLDAFKASGKLSVEDQRWTEARKLFDS 360 + +P+M++ ++SNFERL + + R G+ VA + + G + +E W EARKLFDS Sbjct: 300 RTTKTPTMNVSLASNFERLQWSILNRCGEKVARNMQLLEGEGTIHLEQDEWLEARKLFDS 359 Query: 361 LAVSDEQTCETIAEVYRSSGELLDPHTAIGVRAARECRRSLSVPMVTLGTAHPVKFPEAV 420 LAV D I EV+ SG L+ P+TAIG+RAAR RRSL +PM++L T HP K A Sbjct: 360 LAVDDADAFSCIHEVFAQSGYLVSPNTAIGLRAARVSRRSLLIPMISLATTHPSKIGAAT 419 Query: 421 EKAGI------GQAPALPAHLADLFEREERCTVLPNELAKVQAFV 459 +K+ I G APA E+ CT +PN++ + + V Sbjct: 420 DKSEIFGHLNTGGAPA---------STEQDCTSIPNDIGALTSLV 455 Lambda K H 0.322 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 541 Number of extensions: 17 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 469 Length of database: 467 Length adjustment: 33 Effective length of query: 436 Effective length of database: 434 Effective search space: 189224 Effective search space used: 189224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 51 (24.3 bits)
Align candidate AO356_27150 AO356_27150 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.14428.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.8e-56 175.4 0.0 1.1e-55 175.1 0.0 1.0 1 lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 AO356_27150 threonine synthase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 AO356_27150 threonine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 175.1 0.0 1.1e-55 1.1e-55 26 309 .. 83 395 .. 62 426 .. 0.84 Alignments for each domain: == domain 1 score: 175.1 bits; conditional E-value: 1.1e-55 TIGR00260 26 frspklaeevga..enlyvkelfhgPtlaFKDrglqfvavlltk..alelgnetvlcAtsGdt 84 f+++++ +++ + n +++elfhgP++a+ D+glq ++ +++ ++ +++++++ t Gdt lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 83 FTNNAV-APLYQtaSNEWLLELFHGPSGAYQDFGLQLMSRFINHdlLKSGKKALIVGCTGGDT 144 333333.344333578899***********************975456667779********* PP TIGR00260 85 gaaaaealagkanvkvvvLyPkgkispv.keklvtalaenakvlaikGdFDdaqdlvkeife. 145 gaaa++a++g+ +v +vL P+ ++ +l+ + +n+ ++i Gd+ d+ +l+++ ++ lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 145 GAAAIKAFSGMTGVTLLVLHPSKELTAEdRRRLLSVEEGNVINIEIAGDYSDCHRLCEQFLKq 207 ***********************99977899*******************************5 PP TIGR00260 146 .dkeklklnsvNsinparieaqk.tyafeiveqlgkespdkvvvpvpsgnfgailkGflekke 206 ++ + l+s Ns+ + r++a y+++++ qlg + +v+++vp gnfga+++G+ +k++ lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 208 nNDAQQHLVSFNSVHWVRVLAHLsFYFYSAL-QLG-APKRQVAFSVPTGNFGALYAGYIAKQM 268 5555999****************55555555.667.68899****************999999 PP TIGR00260 207 lglpieklaiaaegaadivrrflksgdlepkedk.eTlstAmdignpsnverale.larrslg 267 + lpi+ l +a++ + +++fl+s l+ +++ T + m+++ sn+er+ + + +r ++ lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 269 G-LPIRQLIVATNAN-AGLHHFLQSN-LYSRNEIrTTKTPTMNVSLASNFERLQWsILNRCGE 328 9.**99999888887.99*****999.666666659999****************86666665 PP TIGR00260 268 nledlke.........................svsdeeileaikklaeeegyllephtavava 305 ++ + +v d++ + i+++ ++ gyl+ p ta++++ lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 329 KVARNMQllegegtihleqdewlearklfdslAVDDADAFSCIHEVFAQSGYLVSPNTAIGLR 391 55444345667***************************************************9 PP TIGR00260 306 alkk 309 a + lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27150 392 AARV 395 9764 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (467 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 12.33 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory