GapMind for Amino acid biosynthesis


Protein Pf6N2E2_2700 in Pseudomonas fluorescens FW300-N2E2

Annotation: FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700

Length: 324 amino acids

Source: pseudo6_N2E2 in FitnessBrowser

Candidate for 4 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-cysteine biosynthesis cysK hi cysteine synthase (EC (characterized) 71% 100% 448.7
L-cysteine biosynthesis cysK hi cysK: cysteine synthase A (EC (TIGR01139) 99% 449.1
L-cysteine biosynthesis PSSH med O-phosphoserine sulfhydrylase monomer (EC; EC (characterized) 43% 99% 231.5 cysteine synthase (EC 71% 448.7
L-cysteine biosynthesis CBS lo Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC (characterized) 43% 67% 237.7 cysteine synthase (EC 71% 448.7
L-cysteine biosynthesis Mt_cysM lo [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase (EC; O-phosphoserine sulfhydrylase (EC (characterized) 34% 95% 175.3 cysteine synthase (EC 71% 448.7

Sequence Analysis Tools

View Pf6N2E2_2700 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...



This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.



Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory