Alignments for a candidate for glyA in Pseudomonas fluorescens FW300-N2E2

GapMind for Amino acid biosynthesis

Alignments for a candidate for glyA in Pseudomonas fluorescens FW300-N2E2

Align glycine hydroxymethyltransferase (EC 2.1.2.1) (characterized)
to candidate Pf6N2E2_5295 Serine hydroxymethyltransferase (EC 2.1.2.1)

Query= BRENDA::B4ECY9
         (415 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5295
          Length = 412

 Score =  540 bits (1392), Expect = e-158
 Identities = 263/408 (64%), Positives = 326/408 (79%), Gaps = 1/408 (0%)

Query: 8   TIANVDPEIFAAIEQENRRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYG 67
           ++ N DP +   I++E  RQE H+ELIASENY S  V+ AQGS LTNKYAEGYPGKRYYG
Sbjct: 2   SLQNFDPTLARLIDRERNRQETHLELIASENYVSEEVLQAQGSVLTNKYAEGYPGKRYYG 61

Query: 68  GCEYVDVVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVFFAMLKPGDTIMGMSLAHGGH 127
           GC+ VD +E LAI+R ++LF  E  NVQP+SGSQANQ VF A+L+PGDTI+GMSLAHGGH
Sbjct: 62  GCKVVDEIENLAIERARKLFNCEYVNVQPHSGSQANQAVFLAVLEPGDTILGMSLAHGGH 121

Query: 128 LTHGSPVNMSGKWFNVVSYGLN-ENEDIDYDAAEKLANEHKPKLIVAGASAFALKIDFER 186
           LTHG+ VN SGK +   SYGL+ E E +DY+  E LA EH+PK+I+AGASA++  IDF+R
Sbjct: 122 LTHGASVNFSGKIYRAFSYGLDTETETLDYEEMEALAREHRPKMIIAGASAYSRTIDFQR 181

Query: 187 LAKIAKSVGAYLMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVILMKAEY 246
             KI   +GAYLMVDMAHYAGLIAAGVYP+PV  ADF+T+TTHK+LRGPRGG+IL KA+Y
Sbjct: 182 FRKICDEIGAYLMVDMAHYAGLIAAGVYPSPVGIADFITSTTHKTLRGPRGGLILAKAQY 241

Query: 247 EKPINSAIFPGIQGGPLMHVIAAKAVAFKEALSPEFKEYQQKVVENARVLAETLVKRGLR 306
              ++  IFP  QGGPLMHVIAAKAVAF EAL   FK YQQ+V++NARV+A+ L +RGLR
Sbjct: 242 GALLDKTIFPVYQGGPLMHVIAAKAVAFNEALGDGFKHYQQRVIDNARVMADVLTRRGLR 301

Query: 307 IVSGRTESHVMLVDLRAKHITGKAAEAALGAAHITVNKNAIPNDPEKPFVTSGIRLGSPA 366
           +VSG T+ H+ L+DLR+ +ITGK AEA L +AHIT+NKNAIPNDP+KP +TSGIR+G+PA
Sbjct: 302 VVSGGTDCHMFLLDLRSMNITGKDAEALLESAHITLNKNAIPNDPQKPAITSGIRIGTPA 361

Query: 367 MTTRGFGPAEAEQVGNLIADVLENPEDAATIERVRAQVAELTKRFPVY 414
           +TTRGFG AE  +V NLIAD+LE P++AA ++  R +V  L + FPVY
Sbjct: 362 LTTRGFGEAECAEVANLIADLLEQPDNAARLDNTRRRVMHLCECFPVY 409


Lambda     K      H
   0.316    0.132    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 519
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 415
Length of database: 412
Length adjustment: 31
Effective length of query: 384
Effective length of database: 381
Effective search space:   146304
Effective search space used:   146304
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis