GapMind for Amino acid biosynthesis

 

Alignments for a candidate for thrB in Pseudomonas fluorescens FW300-N2E2

Align Homoserine kinase (EC:2.7.1.39) (characterized)
to candidate Pf6N2E2_3755 Shikimate kinase I (EC 2.7.1.71)

Query= reanno::Miya:8500213
         (185 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3755
          Length = 163

 Score = 67.4 bits (163), Expect = 1e-16
 Identities = 51/145 (35%), Positives = 68/145 (46%), Gaps = 3/145 (2%)

Query: 28  GAGKTTVGRELALQLGWAHVDTDNLIEATYGTRLQAVADSMDKESFLDVEAGVIRRIGA- 86
           GAGK+T+GR LA +L     D+D  IE   G  +  + D   +  F D E  +I  + A 
Sbjct: 2   GAGKSTIGRLLAKELRLPFKDSDKEIELRTGANIPWIFDKEGEPGFRDREQAMIAELCAF 61

Query: 87  RRTVLSTGGSVVYRHEAMAHLAALGPLVYLDVSLPLILKRIAMNPDRGL--AIAPGQTIE 144
              VL+TGG  V R      L A G +VYL  S+   + R A + +R L     P +T+ 
Sbjct: 62  DGVVLATGGGAVMREANRRALHAGGRVVYLHASVEQQVGRTARDRNRPLLRTADPAKTLR 121

Query: 145 DLYNERIALYRRYATFTVAADALSP 169
           DL   R  LYR  A   V  D   P
Sbjct: 122 DLLELRDPLYREIADLVVETDERPP 146


Lambda     K      H
   0.321    0.137    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 90
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 185
Length of database: 163
Length adjustment: 19
Effective length of query: 166
Effective length of database: 144
Effective search space:    23904
Effective search space used:    23904
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 44 (21.6 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory