GapMind for Amino acid biosynthesis

 

Aligments for a candidate for argD in Desulfovibrio vulgaris Hildenborough

Align Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 (uncharacterized)
to candidate 208056 DVU2559 adenosylmethionine--8-amino-7-oxononanoate aminotransferase

Query= curated2:Q8TUZ5
         (389 letters)



>lcl|MicrobesOnline__882:208056 DVU2559
           adenosylmethionine--8-amino-7-oxononanoate
           aminotransferase
          Length = 542

 Score =  208 bits (529), Expect = 3e-58
 Identities = 140/420 (33%), Positives = 214/420 (50%), Gaps = 52/420 (12%)

Query: 19  PVTLVPGEGARVWDDEGNEYIDLVAGIAVNVLGHCHPAVVEAVKEQVERLIHCSNLYYN- 77
           P  +   +G  + D +GN Y+D V+ +  NV GH HP + EA++ Q++R+ H + L    
Sbjct: 84  PCIIDAADGNHLIDTDGNRYLDGVSSLWTNVHGHRHPHIDEAIRRQLDRVAHSTLLGLGG 143

Query: 78  EPQAEAARLLAEAAPKDLNKVFFCNSGTESVECAIKLARKF--------TGCTKFIAFEG 129
            P  E A  L   AP  L +VF+ +SG+ +VE A+K+A ++           T+ +AF  
Sbjct: 144 TPSIELAARLTAIAPAGLTRVFYSDSGSTAVEAALKIAFQYHRQAPEGDARRTRVMAFSN 203

Query: 130 GFHGRTMGALSATWKPEFREPFEPLVPEFEHVP--------------------YGDVNAV 169
            +HG T+G++S      F   + PL+ +    P                     G+V  +
Sbjct: 204 AYHGDTIGSVSLGGMSLFHGIYGPLLFDPVRAPAPHCYRCPADLRPETCGMACLGEVERL 263

Query: 170 EKAIDDDTAAVIVEP-VQGEAGVRIPPEGFLRELRELCDEHGLLLIVDEVQSGMGRTGQF 228
            +    +  AV+VEP VQG AG+ + P G+LR LR+LCD HG+ ++ DEV  G G+TG  
Sbjct: 264 MRHHGHELCAVVVEPLVQGAAGMLVQPRGWLRGLRDLCDRHGVFMVADEVAVGFGKTGTM 323

Query: 229 FAFEHEDVLPDIVCLAKGLGGG-VPVGATIAREEVAEAFEPG-------DHGSTFGGNPL 280
           FA E E V+PD++CLAKG+ GG +P+ AT+  E + + F  G        HG T+ GN L
Sbjct: 324 FACEQEGVVPDMLCLAKGITGGYLPLAATLVTEHIHDGFLGGYADFRTFFHGHTYTGNAL 383

Query: 281 ACAAVCAAVSTVLEENLPEAAERKGKLAMRILSEAEDV--VEEVRGRGLMMGVEVGDDER 338
           ACAA  A++    EE   E    + +    +L+   D+  V ++R  G+M G+E+  D  
Sbjct: 384 ACAAALASLDVFEEERTLETLRPRIERLATLLAPLNDLPHVGDIRRVGVMTGIELVADRE 443

Query: 339 AKD-----------VAREMLDRGALVNVTSGDVIRLVPPLVIGEDELEKALAELADALRA 387
            +            V  E   RG +V    GDV+ L+PPL I E ELE  +  +  A+ A
Sbjct: 444 TRTPYRPEERIGHRVTLEARRRGVIVR-PLGDVMVLMPPLSITETELETLVHTVRGAIIA 502


Lambda     K      H
   0.318    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 502
Number of extensions: 36
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 389
Length of database: 542
Length adjustment: 33
Effective length of query: 356
Effective length of database: 509
Effective search space:   181204
Effective search space used:   181204
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory