Alignments for a candidate for trpA in Desulfovibrio vulgaris Hildenborough

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpA in Desulfovibrio vulgaris Hildenborough

Align Tryptophan synthase alpha chain; EC 4.2.1.20 (characterized, see rationale)
to candidate 209407 DVU0471 tryptophan synthase, alpha subunit

Query= uniprot:M4NLA4
         (266 letters)



>MicrobesOnline__882:209407
          Length = 257

 Score =  176 bits (446), Expect = 4e-49
 Identities = 105/250 (42%), Positives = 143/250 (57%), Gaps = 2/250 (0%)

Query: 2   SRIDRRFAALKAANRTGLIPFVTAGDPSPEHMVALMHALVDAGADLIELGVPFSDPMADG 61
           SR++RR    +AA R  LIPF+TAG P+ E     + AL  AGAD+IE+GVPFSDP+ADG
Sbjct: 4   SRLERRIREAQAAGRPALIPFLTAGFPTKERFWDELEALDAAGADIIEVGVPFSDPVADG 63

Query: 62  PVIQHASERAIAKGVGLADVLGWVAAFRQHDADTPIVLMGYLNPIETHGYARFAGEAVQA 121
           PV+  AS+RA+  GV L  ++  +AA R+      +VLMGYLNP   +G+ RF  +A  A
Sbjct: 64  PVVAAASQRALESGVTLRWIMDGLAA-RKGRLRAGLVLMGYLNPFMQYGFERFVSDAADA 122

Query: 122 GVDGVLLVDCPLEESAVLQPLRDA-GLQRILLAAPTTEPSRMAQLCGSAEGFLYYVSFAG 180
           GV G ++ D PL+E A L+ L  A  +  I L  P T   RM +    A G++Y VS  G
Sbjct: 123 GVAGCIIPDLPLDEDADLRALLAARDMDLIALVGPNTGEGRMREYAAVASGYVYVVSVMG 182

Query: 181 ITGAAHLSTGDIAARVADVRARAKAPVAVGFGIRDAASAKAIAGFADAVVIGSALVDRLA 240
            TG       ++A  +A  R     PVA+GFGI   A  + ++   DAV+ GSAL+  L 
Sbjct: 183 TTGVRDGLPVEVADTLARARQCFSIPVALGFGISRPAQLEGLSHPPDAVIFGSALLRHLD 242

Query: 241 GATDAGEITR 250
              DA    +
Sbjct: 243 AGGDAASFMK 252


Lambda     K      H
   0.322    0.136    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 217
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 266
Length of database: 257
Length adjustment: 25
Effective length of query: 241
Effective length of database: 232
Effective search space:    55912
Effective search space used:    55912
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

Align candidate 209407 DVU0471 (tryptophan synthase, alpha subunit)
to HMM TIGR00262 (trpA: tryptophan synthase, alpha subunit (EC 4.2.1.20))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00262.hmm
# target sequence database:        /tmp/gapView.19605.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00262  [M=256]
Accession:   TIGR00262
Description: trpA: tryptophan synthase, alpha subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                       -----------
      7e-66  207.7   0.0    8.1e-66  207.5   0.0    1.0  1  lcl|MicrobesOnline__882:209407  DVU0471 tryptophan synthase, alp


Domain annotation for each sequence (and alignments):
>> lcl|MicrobesOnline__882:209407  DVU0471 tryptophan synthase, alpha subunit
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  207.5   0.0   8.1e-66   8.1e-66       6     237 ..      15     244 ..      10     256 .. 0.94

  Alignments for each domain:
  == domain 1  score: 207.5 bits;  conditional E-value: 8.1e-66
                       TIGR00262   6 kkeekafvpFvtagdPdleksleiiktlvkaGadalElGvpfsDPlaDGptiqaaelRAlkagvkvekalellkkvrek 84 
                                     +++  a++pF+tag P+ e+  + ++ l  aGad++E+GvpfsDP+aDGp++ aa++RAl++gv+++ +++ l++ +  
  lcl|MicrobesOnline__882:209407  15 AAGRPALIPFLTAGFPTKERFWDELEALDAAGADIIEVGVPFSDPVADGPVVAAASQRALESGVTLRWIMDGLAARK-G 92 
                                     566779*************************************************************9998776655.5 PP

                       TIGR00262  85 asniPivlltyynlifkkgveeFyakakeagvdgvlvaDlPleeaddlleaakkhgvkqiflvaPtaeeerlkkiaeks 163
                                     +  + +vl+ y n+ +++g e F++ a++agv g +++DlPl+e +dl+   + ++++ i lv P++ e r+++ a  +
  lcl|MicrobesOnline__882:209407  93 RLRAGLVLMGYLNPFMQYGFERFVSDAADAGVAGCIIPDLPLDEDADLRALLAARDMDLIALVGPNTGEGRMREYAAVA 171
                                     599**************************************************************************** PP

                       TIGR00262 164 eGfvYlvsvaGvtgarerveeevkelikkvkalskkPvlvGFGiskkeqvkelkelgadgvivGsAlvkiieek 237
                                     +G+vY+vsv+G tg+r+ +  ev + +++++++ ++Pv++GFGis++ q++ l +   d+vi GsAl++ ++  
  lcl|MicrobesOnline__882:209407 172 SGYVYVVSVMGTTGVRDGLPVEVADTLARARQCFSIPVALGFGISRPAQLEGLSHP-PDAVIFGSALLRHLDAG 244
                                     ****************************************************9887.889*********99877 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (256 nodes)
Target sequences:                          1  (257 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 9.10
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis