Align Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 (characterized)
to candidate WP_011383030.1 AMB_RS03035 aspartate-semialdehyde dehydrogenase
Query= SwissProt::P23247 (337 letters) >NCBI__GCF_000009985.1:WP_011383030.1 Length = 338 Score = 332 bits (850), Expect = 1e-95 Identities = 173/334 (51%), Positives = 233/334 (69%), Gaps = 3/334 (0%) Query: 5 FNVAIFGATGAVGETMLEVLQEREFPVDELFLLASERSEGKTYRFNGKTVRVQNVEEFDW 64 + VA+ GATG VG ML++L +R+FP DE+ LASERS G+ + K ++ +++ FD+ Sbjct: 3 YKVAVIGATGNVGRAMLQILVDRKFPADEVVALASERSVGREVSYGDKVLKCKDLATFDF 62 Query: 65 SQVHIALFSAGGELSAKWAPIAAEAGVVVIDNTSHFRYDYDIPLVVPEVNPEAIAEFRNR 124 IAL S G ++SA +P AA AG VVIDNTSHFR + D+PLVVPEVNP+AIA+++ R Sbjct: 63 KGCDIALSSPGAKVSAIHSPRAAAAGCVVIDNTSHFRMEPDVPLVVPEVNPDAIAQYKKR 122 Query: 125 NIIANPNCSTIQMLVALKPIYDAVGIERINVTTYQSVSGAGKAGIDELAGQTAKLLNGYP 184 IIANPNCSTIQM+VALKP++ I+R+ V+TYQSVSGAGK G+DEL QT +L Sbjct: 123 GIIANPNCSTIQMVVALKPLHKLGKIKRVVVSTYQSVSGAGKEGMDELYDQTKGILVHDA 182 Query: 185 AETNTFSQQIAFNCIPQIDQFMDNGYTKEEMKMVWETQKIFNDPSIMVNPTCVRVPVFYG 244 + F++QIAFN IPQID FM++G TKEE KMV ET KI DP I VN TCVRVPVF Sbjct: 183 IKPQKFAKQIAFNLIPQIDVFMEDGSTKEEWKMVVETHKIL-DPDIAVNATCVRVPVFVS 241 Query: 245 HAEAVHVETRAPIDAEQVMDMLEQTDGIELFRGADFPTQVR--DAGGKDHVLVGRVRNDI 302 H+E+++VE P+ + + L + +G+ + + + D G+D V + R+R D Sbjct: 242 HSESINVEFERPVSVAEATEALREAEGVVVMDTREPGGYITPLDTTGEDPVYISRIRKDP 301 Query: 303 SHHSGINLWVVADNVRKGAATNAVQIAELLVRDY 336 + +G++ W VADN+RKGAA NAVQIAE+L+RDY Sbjct: 302 TVKNGLSFWCVADNLRKGAALNAVQIAEVLIRDY 335 Lambda K H 0.319 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 320 Number of extensions: 12 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 337 Length of database: 338 Length adjustment: 28 Effective length of query: 309 Effective length of database: 310 Effective search space: 95790 Effective search space used: 95790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate WP_011383030.1 AMB_RS03035 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01296.hmm # target sequence database: /tmp/gapView.7585.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01296 [M=339] Accession: TIGR01296 Description: asd_B: aspartate-semialdehyde dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4e-145 469.1 0.5 4.5e-145 468.9 0.5 1.0 1 lcl|NCBI__GCF_000009985.1:WP_011383030.1 AMB_RS03035 aspartate-semialdehy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000009985.1:WP_011383030.1 AMB_RS03035 aspartate-semialdehyde dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 468.9 0.5 4.5e-145 4.5e-145 1 338 [. 4 333 .. 4 334 .. 0.99 Alignments for each domain: == domain 1 score: 468.9 bits; conditional E-value: 4.5e-145 TIGR01296 1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsa 69 +va++GatG+vG+ +l++L +r+fp+d++v+lasers G++v + +k l+ +++ +++f+g dial s lcl|NCBI__GCF_000009985.1:WP_011383030.1 4 KVAVIGATGNVGRAMLQILVDRKFPADEVVALASERSVGREVSYGDKVLKCKDLATFDFKGCDIALSSP 72 69******************************************************************* PP TIGR01296 70 GgsvskefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkp 138 G+ vs p+aa+ag++viDnts fr+++dvPLvvpevn + +++ kk+giianPnCstiq+vv+Lkp lcl|NCBI__GCF_000009985.1:WP_011383030.1 73 GAKVSAIHSPRAAAAGCVVIDNTSHFRMEPDVPLVVPEVNPDAIAQYKKRGIIANPNCSTIQMVVALKP 141 ********************************************************************* PP TIGR01296 139 lkdeaklkrvvvstYqavsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplidkl 207 l++ k+krvvvstYq+vsGaGk+g++eL++qtk +l+ ++ ++kfakqiafn+ip+id + lcl|NCBI__GCF_000009985.1:WP_011383030.1 142 LHKLGKIKRVVVSTYQSVSGAGKEGMDELYDQTKGILVHDAIK-------PQKFAKQIAFNLIPQIDVF 203 ************************************9876655.......6****************** PP TIGR01296 208 kedGytkeelkllfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgvvv 276 +edG tkee k++ et+kil+ +d+ v atcvrvPvf++hses+++efe+++sv e+ e L+ea+gvvv lcl|NCBI__GCF_000009985.1:WP_011383030.1 204 MEDGSTKEEWKMVVETHKILD-PDIAVNATCVRVPVFVSHSESINVEFERPVSVAEATEALREAEGVVV 271 *********************.*********************************************** PP TIGR01296 277 iddpsenlyptPleavgkdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiaellik 338 +d + y+tPl+++g+d v+++rirkD + ++gl+ ++vaDnlrkGaalnavqiae li+ lcl|NCBI__GCF_000009985.1:WP_011383030.1 272 MDTREPGGYITPLDTTGEDPVYISRIRKDPTVKNGLSFWCVADNLRKGAALNAVQIAEVLIR 333 ***********************************************************997 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (339 nodes) Target sequences: 1 (338 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 8.85 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory