Alignments for a candidate for thrC in Magnetospirillum magneticum AMB-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrC in Magnetospirillum magneticum AMB-1

Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate WP_011385748.1 AMB_RS17140 threonine synthase

Query= BRENDA::Q8YFS0
         (463 letters)



>NCBI__GCF_000009985.1:WP_011385748.1
          Length = 467

 Score =  493 bits (1268), Expect = e-144
 Identities = 253/463 (54%), Positives = 324/463 (69%), Gaps = 1/463 (0%)

Query: 1   MKYVSTRGEAPVLGFSDALLAGLARDGGLYLPQEYPQFTAEQIRALRGKSYVEVALAVLT 60
           MKYVSTRG AP L F D LLAGLARDGGLYLP+ +P F+A  IRA+RG SY E+A+ V+ 
Sbjct: 1   MKYVSTRGAAPALDFDDVLLAGLARDGGLYLPESWPVFSAADIRAMRGLSYAELAVRVMR 60

Query: 61  PFTGGEIPAADFERMVREAYGTFRHDAVCPLVQTDANEFVLELFHGPTLAFKDVAMQLLA 120
           PF  G +  A+  R+  E+YG F H AV PL Q   N++V+ELFHGPTLAFKD A+QL+ 
Sbjct: 61  PFVEGCLTEAELTRLCAESYGAFAHPAVAPLKQLGHNQWVMELFHGPTLAFKDYALQLVG 120

Query: 121 RMMDYVLAQRGERATIVGATSGDTGGAAIEAFGGRDNTDIFILFPNGRVSPVQQRQMTSS 180
           R+ D+VL ++G++ TIVGATSGDTG AAIEA   R   DI IL P+GRVS VQ+RQMT+ 
Sbjct: 121 RLFDHVLKKKGQKVTIVGATSGDTGSAAIEACRDRAAVDIVILHPHGRVSEVQRRQMTTV 180

Query: 181 GFSNVHALSIEGNFDDCQNLVKGMFNDLEFCDALSLSGVNSINWARIMPQVVYYFTAALS 240
             SNV  ++++G FDDCQ+LVK +FND  F D ++LS VNSINWAR+M Q+VYYF A ++
Sbjct: 181 LSSNVRNVAVDGTFDDCQDLVKALFNDAAFRDEMNLSAVNSINWARVMAQIVYYFAAGVA 240

Query: 241 LGAPDRAVSFTVPTGNFGDIFAGYVAKRMGLPIEQLIIATNDNDILSRTLESGAYEMRGV 300
           LGAPD  +SF+VPTGNFG++FAGY AK MGLP+E+LII +N NDIL+R  E G  +  GV
Sbjct: 241 LGAPDVPMSFSVPTGNFGNVFAGYAAKLMGLPVEKLIIGSNTNDILTRFFEGGVMKADGV 300

Query: 301 AQTTSPSMDIQISSNFERLLFEAHGRDAAAVRGLMQGLKQSGGFTISEKPLSAIRSEFSA 360
             T SPSMDIQ+SSNFERL+F A G D AAV  LM   +++G   + +   +A+R  F  
Sbjct: 301 VPTLSPSMDIQVSSNFERLMFLALGGDGAAVAKLMDDFRKNGAMVMPQGAWTAMREVFEG 360

Query: 361 GRSTVDETAATIESVLSKDGYLLDPHSAIGVKVA-REKASGTAPMVVLATAHPAKFPDAV 419
            R   + T A + S+    G  LDPHS IGV  A +  A+    MV LATAHPAKFPDAV
Sbjct: 361 YRFDDEATLAAMRSLKRSTGETLDPHSVIGVAAALKGHAARDCSMVALATAHPAKFPDAV 420

Query: 420 KAACGVEPQLPAWLCDLMQRKESFTVLHNELKIVEEYVRHHSR 462
           + A G+ P LP  + DL +R E    + N+  ++++YVR  +R
Sbjct: 421 EKATGIRPGLPPHMADLFERTERLDRMGNDADLLKQYVRAFAR 463


Lambda     K      H
   0.320    0.135    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 551
Number of extensions: 18
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 463
Length of database: 467
Length adjustment: 33
Effective length of query: 430
Effective length of database: 434
Effective search space:   186620
Effective search space used:   186620
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

Align candidate WP_011385748.1 AMB_RS17140 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00260.hmm
# target sequence database:        /tmp/gapView.31869.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00260  [M=340]
Accession:   TIGR00260
Description: thrC: threonine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
      2e-86  276.2   0.0    2.8e-86  275.7   0.0    1.1  1  lcl|NCBI__GCF_000009985.1:WP_011385748.1  AMB_RS17140 threonine synthase


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000009985.1:WP_011385748.1  AMB_RS17140 threonine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  275.7   0.0   2.8e-86   2.8e-86      12     334 ..      69     426 ..      57     430 .. 0.91

  Alignments for each domain:
  == domain 1  score: 275.7 bits;  conditional E-value: 2.8e-86
                                 TIGR00260  12 ekdlvdlaegstelfrspkla..eevgaenlyvkelfhgPtlaFKDrglqfvavlltkalelgne..tv 76 
                                               e++l  l++ s+ +f+ p++a  +++g  n +v elfhgPtlaFKD +lq v+ l+ ++l+++ +  t+
  lcl|NCBI__GCF_000009985.1:WP_011385748.1  69 EAELTRLCAESYGAFAHPAVAplKQLGH-NQWVMELFHGPTLAFKDYALQLVGRLFDHVLKKKGQkvTI 136
                                               789999*************998899999.******************************98887668** PP

                                 TIGR00260  77 lcAtsGdtgaaaaealagkanvkvvvLyPkgkispv.keklvtalaenakvlaikGdFDdaqdlvkeif 144
                                               ++AtsGdtg+aa+ea + +a v++v+L P+g++s v   +++t+l +n++ +a++G+FDd+qdlvk++f
  lcl|NCBI__GCF_000009985.1:WP_011385748.1 137 VGATSGDTGSAAIEACRDRAAVDIVILHPHGRVSEVqRRQMTTVLSSNVRNVAVDGTFDDCQDLVKALF 205
                                               ************************************99******************************* PP

                                 TIGR00260 145 edke...klklnsvNsinparieaqktyafeiveqlgkespd.kvvvpvpsgnfgailkGflekkelgl 209
                                               +d     +++l++vNsin+ar+ aq++y+f +  +lg  +pd  + ++vp gnfg++++G+ +k ++ l
  lcl|NCBI__GCF_000009985.1:WP_011385748.1 206 NDAAfrdEMNLSAVNSINWARVMAQIVYYFAAGVALG--APDvPMSFSVPTGNFGNVFAGYAAKLMG-L 271
                                               9776788******************************..6662589**************8888777.* PP

                                 TIGR00260 210 pieklaiaaegaadivrrflksgdlepkedkeTlstAmdignpsnverale.larrslgnledlke... 274
                                               p+ekl i ++ + di++rf ++g ++    + Tls++mdi  +sn+er+++ +    ++ + +l     
  lcl|NCBI__GCF_000009985.1:WP_011385748.1 272 PVEKLIIGSNTN-DILTRFFEGGVMKADGVVPTLSPSMDIQVSSNFERLMFlALGGDGAAVAKLMDdfr 339
                                               ***********9.**********66666666********************444444555555544899 PP

                                 TIGR00260 275 ......................svsdeeileaikklaeeegyllephtavavaalkklvekg.vs.... 316
                                                                     +  de++l a++ l ++ g +l+ph+ ++vaa +k +++   s    
  lcl|NCBI__GCF_000009985.1:WP_011385748.1 340 kngamvmpqgawtamrevfegyRFDDEATLAAMRSLKRSTGETLDPHSVIGVAAALKGHAARdCSmval 408
                                               9********************************************************999987334568 PP

                                 TIGR00260 317 atadpaKFeevvealtgn 334
                                               ata+paKF+++ve  tg 
  lcl|NCBI__GCF_000009985.1:WP_011385748.1 409 ATAHPAKFPDAVEKATGI 426
                                               *************88885 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (340 nodes)
Target sequences:                          1  (467 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 9.11
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis