Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate WP_011385867.1 AMB_RS17740 acetolactate synthase 3 large subunit
Query= metacyc::MONOMER-18810 (585 letters) >NCBI__GCF_000009985.1:WP_011385867.1 Length = 588 Score = 644 bits (1660), Expect = 0.0 Identities = 321/569 (56%), Positives = 416/569 (73%), Gaps = 6/569 (1%) Query: 19 MIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYAR 78 + GAEIL+ AL ++GVE ++GYPGGAVL IYDEL KQ + H+LVRHEQAAVHAA+GYAR Sbjct: 7 LTGAEILLKALVDQGVEVIFGYPGGAVLPIYDELFKQNRLRHVLVRHEQAAVHAAEGYAR 66 Query: 79 ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITR 138 +TGKVG LVTSGPG TNAVTG+A A DS+P+V +TG VPTH IG DAFQE D GITR Sbjct: 67 STGKVGCVLVTSGPGATNAVTGLADAQCDSVPLVCLTGQVPTHLIGNDAFQEADITGITR 126 Query: 139 PIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDMRSY 198 P KHN+LVKDV DLA T+ +AF +A +GRPGPV+VD+PKDV + +Y+ P + + Y Sbjct: 127 PCTKHNYLVKDVADLARTVHEAFHVARSGRPGPVLVDLPKDVVQAKGEYQPPSKLKSK-Y 185 Query: 199 NPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLAN--ASDELRQLAALTGHPVTNTLMG 256 P KG I KA+ ++ A++P Y GGGVV + A L Q +TG+P T TLMG Sbjct: 186 KPQVKGDLKAIEKAIEMIAHAKKPIFYVGGGVVNSGPAACQLLTQFVRMTGYPCTLTLMG 245 Query: 257 LGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKIIH 316 LGAFPG+ F+GMLGMHGT EANMAM +CD++I IGARFDDRV G F+ +++K IH Sbjct: 246 LGAFPGSDPLFLGMLGMHGTVEANMAMHDCDLMINIGARFDDRVTGKLDGFSPKSKK-IH 304 Query: 317 IDIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALAKWWEQIEQWRSVDC 376 +DIDPSSI+K V VD+PIVG+ VL+++I KA + +AL WW +I+QWR DC Sbjct: 305 VDIDPSSINKNVAVDLPIVGDCAHVLEDMIKVWKAKQSRVDDKALKAWWAKIDQWRGADC 364 Query: 377 LKYDRSSEIIKPQYVVEKIWELTK-GDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLG 435 L ++ S++IIKPQY +++++E+T+ + + C++VGQHQMWAAQ KFD P W+ SGGLG Sbjct: 365 LSFENSNKIIKPQYAIQRLYEMTRHRNPYFCTEVGQHQMWAAQHLKFDLPLHWLTSGGLG 424 Query: 436 TMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMV 495 TMG GLP AMG++ A PE V+ I GE SIQM IQE++T +Q+ PVKI LNN YLGMV Sbjct: 425 TMGYGLPSAMGVQMAHPEALVINIAGEASIQMNIQEMATLVQHRLPVKIVILNNQYLGMV 484 Query: 496 RQWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFL 555 RQWQE+ + RYS S+M PDFVKLAEA+G VG+R +K +V+ A++E + DR V L Sbjct: 485 RQWQELIHGGRYSESHMIHHPDFVKLAEAFGAVGLRADKPGEVDGAIKEMIAV-DRPVIL 543 Query: 556 DFQTDPTENVWPMVQAGKGISEMLLGAED 584 + +TD +ENV+PM+ G +EM+LG ED Sbjct: 544 ELRTDASENVFPMIMPGMAHNEMVLGPED 572 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 962 Number of extensions: 30 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 588 Length adjustment: 37 Effective length of query: 548 Effective length of database: 551 Effective search space: 301948 Effective search space used: 301948 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate WP_011385867.1 AMB_RS17740 (acetolactate synthase 3 large subunit)
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.11657.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.4e-246 804.5 0.3 2.7e-246 804.3 0.3 1.0 1 lcl|NCBI__GCF_000009985.1:WP_011385867.1 AMB_RS17740 acetolactate synthas Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000009985.1:WP_011385867.1 AMB_RS17740 acetolactate synthase 3 large subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 804.3 0.3 2.7e-246 2.7e-246 1 554 [. 7 567 .. 7 570 .. 0.97 Alignments for each domain: == domain 1 score: 804.3 bits; conditional E-value: 2.7e-246 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydaly.dselehilvrheqaaahaadGyarasGkvGvvl 68 l+gaeil+++l ++gve++fGyPGGavlpiyd+l+ ++ l+h+lvrheqaa+haa+Gyar++GkvG+vl lcl|NCBI__GCF_000009985.1:WP_011385867.1 7 LTGAEILLKALVDQGVEVIFGYPGGAVLPIYDELFkQNRLRHVLVRHEQAAVHAAEGYARSTGKVGCVL 75 68*********************************8999****************************** PP TIGR00118 69 atsGPGatnlvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpei 137 +tsGPGatn+vtg+a+a dsvPlv ltGqv+t+liG+dafqe+di+Git+p+tkh++lvk+++dl+++ lcl|NCBI__GCF_000009985.1:WP_011385867.1 76 VTSGPGATNAVTGLADAQCDSVPLVCLTGQVPTHLIGNDAFQEADITGITRPCTKHNYLVKDVADLART 144 ********************************************************************* PP TIGR00118 138 lkeafeiastGrPGPvlvdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvll 206 ++eaf++a +GrPGPvlvdlPkdv++a+ e++ ++k++ ykp+vkg+ + i+ka+e+i++akkP+ + lcl|NCBI__GCF_000009985.1:WP_011385867.1 145 VHEAFHVARSGRPGPVLVDLPKDVVQAKGEYQPPSKLK-SKYKPQVKGDLKAIEKAIEMIAHAKKPIFY 212 *********************************98877.56**************************** PP TIGR00118 207 vGgGviiae..aseelkelaerlkipvtttllGlGafpedhplalgmlGmhGtkeanlavseadlliav 273 vGgGv+ ++ a + l++++ ++ p t tl+GlGafp +pl+lgmlGmhGt ean+a++++dl+i + lcl|NCBI__GCF_000009985.1:WP_011385867.1 213 VGGGVVNSGpaACQLLTQFVRMTGYPCTLTLMGLGAFPGSDPLFLGMLGMHGTVEANMAMHDCDLMINI 281 ******88643678999**************************************************** PP TIGR00118 274 GarfddrvtgnlakfapeakiihididPaeigknvkvdipivGdakkvleellkklkee....ekkeke 338 Garfddrvtg+l+ f+p++k ih+didP++i+knv+vd+pivGd+ +vle+++k k++ ++k + lcl|NCBI__GCF_000009985.1:WP_011385867.1 282 GARFDDRVTGKLDGFSPKSKKIHVDIDPSSINKNVAVDLPIVGDCAHVLEDMIKVWKAKqsrvDDKALK 350 *****************************************************9877765566555556 PP TIGR00118 339 .WlekieewkkeyilkldeeeesikPqkvikelskllkd.eaivttdvGqhqmwaaqfyktkkprkfit 405 W++ki++w+ +l+++++++ ikPq+ i++l++++++ + + t+vGqhqmwaaq++k++ p +++t lcl|NCBI__GCF_000009985.1:WP_011385867.1 351 aWWAKIDQWRGADCLSFENSNKIIKPQYAIQRLYEMTRHrNPYFCTEVGQHQMWAAQHLKFDLPLHWLT 419 7***********************************998689*************************** PP TIGR00118 406 sgGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivilnnellGmvkqWq 474 sgGlGtmG+GlP+a+G+++a+pe+ v++++G++s+qmn+qe++t+v+ +pvkivilnn++lGmv+qWq lcl|NCBI__GCF_000009985.1:WP_011385867.1 420 SGGLGTMGYGLPSAMGVQMAHPEALVINIAGEASIQMNIQEMATLVQHRLPVKIVILNNQYLGMVRQWQ 488 ********************************************************************* PP TIGR00118 475 elfyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeeevlPm 543 el++ +ryse+++ + +pdfvklaea+G++g+r kp e++ ++ke++++++pv+l+++ d +e+v+Pm lcl|NCBI__GCF_000009985.1:WP_011385867.1 489 ELIHGGRYSESHMIH-HPDFVKLAEAFGAVGLRADKPGEVDGAIKEMIAVDRPVILELRTDASENVFPM 556 **************5.***************************************************** PP TIGR00118 544 vapGagldelv 554 ++pG++ +e+v lcl|NCBI__GCF_000009985.1:WP_011385867.1 557 IMPGMAHNEMV 567 **********8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (588 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 10.20 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory