Protein 350994 in Bacteroides thetaiotaomicron VPI-5482
Annotation: FitnessBrowser__Btheta:350994
Length: 238 amino acids
Source: Btheta in FitnessBrowser
Candidate for 16 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-asparagine catabolism | bgtA | med | ATPase (characterized, see rationale) | 41% | 79% | 146 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| L-aspartate catabolism | bgtA | med | ATPase (characterized, see rationale) | 41% | 79% | 146 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| D-maltose catabolism | thuK | lo | ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) | 42% | 60% | 144.8 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| sucrose catabolism | thuK | lo | ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) | 42% | 60% | 144.8 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| trehalose catabolism | thuK | lo | ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) | 42% | 60% | 144.8 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| D-maltose catabolism | malK | lo | ABC-type maltose transporter (subunit 3/3) (EC 7.5.2.1) (characterized) | 40% | 57% | 140.2 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| xylitol catabolism | Dshi_0546 | lo | ABC transporter for Xylitol, ATPase component (characterized) | 38% | 64% | 139 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| L-proline catabolism | opuBA | lo | BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized) | 36% | 54% | 136.3 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| N-acetyl-D-glucosamine catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 39% | 62% | 136 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| D-glucosamine (chitosamine) catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 39% | 62% | 136 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| L-arabinose catabolism | xacJ | lo | Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale) | 37% | 54% | 132.9 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| glycerol catabolism | glpT | lo | GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized) | 34% | 57% | 119.4 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| L-histidine catabolism | hutV | lo | ABC transporter for L-Histidine, ATPase component (characterized) | 32% | 86% | 119 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| citrate catabolism | fecE | lo | iron(III) dicitrate transport ATP-binding protein FecE (characterized) | 31% | 85% | 115.9 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| D-mannose catabolism | TM1749 | lo | TM1749, component of Probable mannose/mannoside porter. Induced by beta-mannan (Conners et al., 2005). Regulated by mannose-responsive regulator manR (characterized) | 33% | 73% | 114.8 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
| glycerol catabolism | glpS | lo | GlpS, component of Glycerol uptake porter, GlpSTPQV (characterized) | 34% | 53% | 104 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 46% | 211.1 |
Sequence Analysis Tools
View 350994 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MIKTEKLSMLFTTEEVQTKALNEVTLHVEQGEFVAIMGPSGCGKSTLLNILGTLDSPTSG
SYFFEGKQVDKMNENQLTALRKNNLGFIFQSFNLIDELTVYENVELPLVYMGIKTAQRKE
KVNKVLEKVNLLHRANHYPQQLSGGQQQRVAIARAVVTDCKLLLADEPTGNLDSVNGVEV
MELLSELNRQGTTIIIVTHSQRDATYAHRIIRLLDGQIVSENINRPLEKSTSSKNEAV
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory