GapMind for catabolism of small carbon sources

 

Protein 351063 in Bacteroides thetaiotaomicron VPI-5482

Annotation: BT1535 ABC transporter ATP-binding protein (NCBI ptt file)

Length: 221 amino acids

Source: Btheta in FitnessBrowser

Candidate for 12 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arabinose catabolism xacJ lo Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale) 37% 55% 137.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
L-arabinose catabolism xacK lo Xylose/arabinose import ATP-binding protein XacK; EC 7.5.2.13 (characterized, see rationale) 36% 56% 135.2 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
D-cellobiose catabolism gtsD lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
D-galactose catabolism PfGW456L13_1897 lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
D-glucose catabolism gtsD lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
lactose catabolism gtsD lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
D-maltose catabolism gtsD lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
sucrose catabolism gtsD lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
trehalose catabolism gtsD lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
L-leucine catabolism livG lo High-affinity branched-chain amino acid transport ATP-binding protein LivG aka B3455, component of Leucine; leucine/isoleucine/valine porter (characterized) 31% 76% 94.4 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
L-phenylalanine catabolism livG lo High-affinity branched-chain amino acid transport ATP-binding protein LivG aka B3455, component of Leucine; leucine/isoleucine/valine porter (characterized) 31% 76% 94.4 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3
L-valine catabolism livG lo High-affinity branched-chain amino acid transport ATP-binding protein LivG aka B3455, component of Leucine; leucine/isoleucine/valine porter (characterized) 31% 76% 94.4 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3

Sequence Analysis Tools

View 351063 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MITLTSLSKIYRTDEIETVALENVNLTVDRGEFLSIMGPSGCGKSTLLNIMGLLDAPTTG
TVEINGTHTEGMKDKQLAAFRNKTLGFVFQSFHLINSLNVMDNVELPLLYRRIGSGERKR
LAKEVLEKVGLSHRMNHFPTQLSGGQCQRVTIARAIIGHPEIILADEPTGNLDSKMGAEV
MELLHRLNKEDGRTIVMVTHNEEQAKQTSRTIRFFDGRQVQ

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory