GapMind for catabolism of small carbon sources

 

Protein H281DRAFT_02458 in Paraburkholderia bryophila 376MFSha3.1

Annotation: H281DRAFT_02458 sarcosine oxidase subunit alpha

Length: 1000 amino acids

Source: Burk376 in FitnessBrowser

Candidate for 4 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-threonine catabolism gcvT lo Aminomethyltransferase; EC 2.1.2.10; Glycine cleavage system T protein (uncharacterized) 31% 94% 156 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; EC 1.5.3.1 48% 747.3
L-arginine catabolism put1 lo proline dehydrogenase (EC 1.5.5.2) (characterized) 30% 74% 126.3 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; EC 1.5.3.1 48% 747.3
L-citrulline catabolism put1 lo proline dehydrogenase (EC 1.5.5.2) (characterized) 30% 74% 126.3 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; EC 1.5.3.1 48% 747.3
L-proline catabolism put1 lo proline dehydrogenase (EC 1.5.5.2) (characterized) 30% 74% 126.3 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; EC 1.5.3.1 48% 747.3

Sequence Analysis Tools

View H281DRAFT_02458 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MSQKDRLPNGGRINRALPLTFTFNGRQYQGYQGDTLASALLANGLHFVARSWKYHRPRGI
VTAGVEEPNAVVQLETGAYTVPNARATEVELYQGLVANSVNAKPSIEKDRMAVNQKLARF
IPAGFYYKTFMWPRKFWPKYEEVIRDAAGLGKAPEQLDADRYDKCFAHCDVLVVGGGPTG
LAAAHAAALSGARVTLVDDQPELGGSLLSCRAEIDGKPALQWVHKIEDELRQMPDVKILT
RSTAFGYQDHNLVTVTQRLTDHLPVSQRKGTRELMWKIRAKRVILATGAHERPIVFGNND
LPGVMLASAVSTYLHRYAVLPGRNAVVFTNNDDGYQCALDLKAAGAQVTVIDPRAGESKG
TLPALARRYGVKVIGGAVITAAHGKLRVASVDIASYANGKTGAKQSELPCDLLAMSGGWS
PVLHLFAQSGGKAHWHNEKACFVPGKAMQAETSVGACNGDFTLGQGIRFAVDAGIEAARA
AGHIVARPNPVQVAEISEAKLEPLWLVGGRELATRGPKQFVDFQNDVSAADIFLAAREGF
ESVEHVKRYTAMGFGTDQGKLGNINGMAILAQALGKTIPETGTTTFRPNYTPVTFGTFAG
RELGEFLDPVRKTAVHEWHVENGAAFEDVGNWKRPWYYPKAGEDMHAAVARESLAVRTSV
GILDASTLGKIDIQGPDSAKLLNWVYTNPWSKLEVGKCRYGLMLDENGMIFDDGVTVRLA
DQHYMMTTTTGGAARVLTWLERWLQTEWPDMRVRLASVTDHWATFAVVGPNSRKVLQKVC
HDIDFANAAFPFMSYREGTVAGAASRVMRISFSGELAYEVNVPANVGRAVWEALMAAGAE
FDITPYGTETMHVLRAEKGYIIVGQDTDGSMTPYDLGMGGLVAKSKDFLGKRSLTRSDTA
KPGRKQLVGLLSDDPSFVIPEGSQIVAGPFQGDTAPMLGHVTSSYYSPILKRSIAMAVVK
GGLEKIGETVTVPLSSGKQIAAKITSSVFYDSEGARQHVE

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory