GapMind for catabolism of small carbon sources

 

Protein RR42_RS10790 in Cupriavidus basilensis 4G11

Annotation: FitnessBrowser__Cup4G11:RR42_RS10790

Length: 501 amino acids

Source: Cup4G11 in FitnessBrowser

Candidate for 4 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-proline catabolism proW lo Glycine betaine/proline betaine transport system permease protein ProW (characterized) 35% 52% 119 BilEB aka OpuBB protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) 48% 171.0
L-histidine catabolism hutW lo HutW aka HISW, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) 36% 65% 118.2 BilEB aka OpuBB protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) 48% 171.0
L-proline catabolism hutW lo HutW aka HISW, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) 36% 65% 118.2 BilEB aka OpuBB protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) 48% 171.0
L-histidine catabolism PA5504 lo D-methionine ABC transporter membrane protein, component of L-Histidine uptake porter, MetIQN (characterized) 31% 68% 63.9 BilEB aka OpuBB protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) 48% 171.0

Sequence Analysis Tools

View RR42_RS10790 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Sequence

MCWAVACLSLLATAGTARADTLRVGSKRFTESYILGEVLTQTAAAQASAQHTPGLGNTAI
VFEALKAGSIDLYPDYTGTVSSEILKLPPGASLAQINQALAPLGLAAGIPLGFENTYALA
MEEHKARQLGLAALGDLAAQPQLRLGLSHEFLGRADGWPGLARRYGLPQRPLGLDHGVAY
EALANGQVDAIDIYSTDAKIRKYGLRVLTDDRHYFPRYDAVVLYRLDLPQRFPAAWQALT
GLAGKVSAADMIAMNAAAELDGQSFAAVARAFLAHGNASVADATASARAGLAGTLFGRDT
LRLTCRHLALVLGAVGAASLLGVPLGIVAARRRRLGQVLMATVSVLQTVPSLALLAMLIP
LLGRIGVWPAMVALFLYALLPIVRNTLTGLQQVPAGMREAARALGFTPGQVLRYVELPLS
MPVLLAGIKTAAIISVGTATIAAFVGAGGYGERIATGLALNDHVLLMAGAIPAAVLALLV
QAGFGLGEWYLGRHARSGTVT

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory