GapMind for catabolism of small carbon sources

 

Protein Ga0059261_0201 in Sphingomonas koreensis DSMZ 15582

Annotation: Ga0059261_0201 Na+/H+-dicarboxylate symporters

Length: 455 amino acids

Source: Korea in FitnessBrowser

Candidate for 7 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-asparagine catabolism glt hi Uncharacterized protein (characterized, see rationale) 52% 97% 445.7 The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up 37% 298.9
L-aspartate catabolism glt hi Uncharacterized protein (characterized, see rationale) 52% 97% 445.7 The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up 37% 298.9
L-glutamate catabolism gltP hi proton/sodium-glutamate symport protein GltT (characterized) 41% 94% 317 The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up 37% 298.9
L-malate catabolism dctA med aerobic C4-dicarboxylate transport protein (characterized) 41% 89% 276.2 proton/sodium-glutamate symport protein GltT 41% 317.0
fumarate catabolism dctA med aerobic C4-dicarboxylate transport protein (characterized) 41% 89% 276.2 proton/sodium-glutamate symport protein GltT 41% 317.0
succinate catabolism dctA med aerobic C4-dicarboxylate transport protein (characterized) 41% 89% 276.2 proton/sodium-glutamate symport protein GltT 41% 317.0
acetate catabolism dctA lo Organic acid uptake porter, DctA of 444 aas and 8 - 10 putative TMSs (characterized) 39% 89% 275.8 proton/sodium-glutamate symport protein GltT 41% 317.0

Sequence Analysis Tools

View Ga0059261_0201 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MAKKLTTFILIALVAGAIVGLGLHYGIHNSFGADSAGAEAELKTVAGYFSIVTTIFLRLI
KMIIAPLVFATLVAGIAHMGDTAALGRVGGRAVAWFICASLVSLTLGLILVNLFQPGVGL
NFPLPPVDATSGVEKAAFNLKDFFTHVFPASGIEAMAKNEILQIVIFSLFIGVAITAVGE
KAKPLVSAVEALVHVMLQVTNYVMRFAPIAVFAAVAGTLAERGPAIIGNLAYFMGTFYIA
MFTLWALLIGVCYLIVGKRTGLLVRYIRDPLLLAFSTASSEAAYPRTLEALDRFGVPPRI
ASFVLPLGYSFNLDGSMIYMTFATIFIAQAYGIDLTLGQEITMLLVLMITSKGIAGVPRA
SLVVIAATLGFFDIPEAGLLLILGIDHFLDMGRSATNVVGNAVASAVVAKWEGGRLDPIE
PADIEPPHAPTGGGPAVDEQSFSEFGKPPAGGSQA

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory