GapMind for catabolism of small carbon sources

 

Protein BWI76_RS16795 in Klebsiella michiganensis M5al

Annotation: BWI76_RS16795 amino acid ABC transporter ATPase

Length: 240 amino acids

Source: Koxy in FitnessBrowser

Candidate for 20 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-asparagine catabolism glnQ med Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) 60% 97% 294.7 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-glutamate catabolism gltL med Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) 60% 97% 294.7 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-arginine catabolism artP med Arginine transport ATP-binding protein ArtM (characterized) 58% 100% 275.8 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-histidine catabolism bgtA med BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) 58% 96% 268.1 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-lysine catabolism hisP med BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) 58% 96% 268.1 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-histidine catabolism BPHYT_RS24015 med ABC transporter related (characterized, see rationale) 55% 94% 263.5 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-histidine catabolism hisP med Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 51% 96% 261.5 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-citrulline catabolism AO353_03040 med ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized) 52% 98% 255.4 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-asparagine catabolism bgtA med ATPase (characterized, see rationale) 54% 92% 254.6 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-aspartate catabolism bgtA med ATPase (characterized, see rationale) 54% 92% 254.6 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-asparagine catabolism aatP med ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, ATPase component (characterized) 55% 99% 253.4 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-aspartate catabolism aatP med ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, ATPase component (characterized) 55% 99% 253.4 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-histidine catabolism aapP med ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, ATPase component (characterized) 53% 93% 252.3 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-asparagine catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 52% 93% 249.6 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-aspartate catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 52% 93% 249.6 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-glutamate catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 52% 93% 249.6 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-leucine catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 52% 93% 249.6 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-proline catabolism aapP med AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 52% 93% 249.6 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-asparagine catabolism peb1C med PEB1C, component of Uptake system for glutamate and aspartate (characterized) 53% 99% 245 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3
L-aspartate catabolism peb1C med PEB1C, component of Uptake system for glutamate and aspartate (characterized) 53% 99% 245 Glutamine transport ATP-binding protein GlnQ; EC 7.4.2.- 60% 299.3

Sequence Analysis Tools

View BWI76_RS16795 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MIHINNLHKRFGDSHVLRGISCDIKPQEVVCIIGPSGSGKSTFLRCMNALETVSEGEVEV
NGFAAHDRATDLNKMRESVGMVFQRFNLFPHKTVLENLIMAPMNLRGMARAEAVRLAEEL
LAKVGLSDKRDAWPSSLSGGQQQRVAIARALAMKPSIMLFDEPTSALDPELVGDVLEVMK
NLASEGMTMVIVTHEMGFAREVADRVIFIDQGIIQEEGKPAQIFSAPTNPRTAAFLSKVL

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory