GapMind for catabolism of small carbon sources

 

Protein GFF1918 in Phaeobacter inhibens BS107

Annotation: FitnessBrowser__Phaeo:GFF1918

Length: 414 amino acids

Source: Phaeo in FitnessBrowser

Candidate for 10 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-cellobiose catabolism SMc04259 med ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein (characterized) 38% 97% 295.8 GtsA (GLcE), component of Glucose porter, GtsABCD 36% 232.3
D-cellobiose catabolism gtsA lo Sugar ABC transporter substrate-binding protein (characterized, see rationale) 36% 99% 276.2 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
D-glucose catabolism gtsA lo Sugar ABC transporter substrate-binding protein (characterized, see rationale) 36% 99% 276.2 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
lactose catabolism gtsA lo Sugar ABC transporter substrate-binding protein (characterized, see rationale) 36% 99% 276.2 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
D-maltose catabolism gtsA lo Sugar ABC transporter substrate-binding protein (characterized, see rationale) 36% 99% 276.2 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
sucrose catabolism gtsA lo Sugar ABC transporter substrate-binding protein (characterized, see rationale) 36% 99% 276.2 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
trehalose catabolism gtsA lo Sugar ABC transporter substrate-binding protein (characterized, see rationale) 36% 99% 276.2 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
D-galactose catabolism PfGW456L13_1894 lo ABC transporter for D-Galactose and D-Glucose, periplasmic substrate-binding component (characterized) 35% 100% 227.6 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
D-xylose catabolism gtsA lo ABC transporter for D-Glucose-6-Phosphate, periplasmic substrate-binding component (characterized) 35% 99% 213.4 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8
D-mannose catabolism TT_C0328 lo Glucose-binding protein aka TT_C0328, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 83% 153.7 ABC transporter for D-Cellobiose and D-Salicin, periplasmic substrate-binding protein 38% 295.8

Sequence Analysis Tools

View GFF1918 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MNFKSNLLAGVATLAMTSAATADGIRAEVIHWWVSAGEAAAIKVFADAYTANGGEWIDNG
IGGGGAKTTFVNRLMGGDAPQVGQFNTSREFEEIVDAGLLHSLDAEAEAGNWSALFPGII
DNVVKRDGSYYAVPVNIHGSNWLWHNNQVMADAGLDVPTDWDSFFEAAETLKEAGIIPLA
VGGEAWQERLTFNSVLLSVGGQDLYLRLFEEKDTTALTSDEMKEVFDVYSRLRTLVRETD
PGSPGRSWNDATNMVITGQAAMQIMGDWAKGEFLSAGMTPGVEYGCTPAVIAGSPYMISG
DVFVFPKTGNEEDREAQSLMATTMLDAEVQVAFNNIKGSIPVRPDVDTSQLDVCGQQAIA
LTSNPDTHVGVTQMYISSDLAGALQDVYTQFWNSETMTTEEAITLLSQAYEIAG

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory