GapMind for catabolism of small carbon sources

 

Protein GFF3853 in Phaeobacter inhibens BS107

Annotation: PGA1_78p00170 putative sn-glycerol-3-phosphate transport system permease protein UgpA

Length: 310 amino acids

Source: Phaeo in FitnessBrowser

Candidate for 10 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-cellobiose catabolism SMc04258 hi ABC transporter for D-Cellobiose and D-Salicin, permease component 2 (characterized) 74% 97% 454.9 GtsB (GLcF), component of Glucose porter, GtsABCD 44% 270.4
D-cellobiose catabolism gtsB med GtsB (GLcF), component of Glucose porter, GtsABCD (characterized) 44% 97% 270.4 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
D-glucose catabolism gtsB med GtsB (GLcF), component of Glucose porter, GtsABCD (characterized) 44% 97% 270.4 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
lactose catabolism gtsB med GtsB (GLcF), component of Glucose porter, GtsABCD (characterized) 44% 97% 270.4 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
D-maltose catabolism gtsB med GtsB (GLcF), component of Glucose porter, GtsABCD (characterized) 44% 97% 270.4 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
sucrose catabolism gtsB med GtsB (GLcF), component of Glucose porter, GtsABCD (characterized) 44% 97% 270.4 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
trehalose catabolism gtsB med GtsB (GLcF), component of Glucose porter, GtsABCD (characterized) 44% 97% 270.4 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
D-xylose catabolism gtsB med ABC transporter for D-Glucose-6-Phosphate, permease component 2 (characterized) 43% 97% 268.9 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
D-galactose catabolism PfGW456L13_1895 med ABC transporter for D-Galactose and D-Glucose, permease component 1 (characterized) 43% 97% 267.7 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9
L-arabinose catabolism xacH lo Xylose/arabinose import permease protein XacH (characterized, see rationale) 32% 89% 160.2 ABC transporter for D-Cellobiose and D-Salicin, permease component 2 74% 454.9

Sequence Analysis Tools

View GFF3853 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MTPAPPTGRSGRSPTARPRPPRVLRNLNAKIASVPMILTALVVFMGGTAWTVAHSFTKSR
LLPKWKFVGFDQYERLWSSNRWLISVENLLIYGLCSLVLTMAIGFTLAALLDRKIRFEGA
FRTIFLYPFALSFVVTGLAWQWILNPDFGIQNVVRSWGWESFAFDPLNNPETVIFGVLIA
GLWQGSGFVMVIMLAGLRGIDEDIWKAARVDGIGVTKTYVRVIIPMMRPVFVTALVIIAS
GIIKLYDLVVAQTNGGPGISSEVPAKYVINYMFEAQNLGQGFAASTMMLLSVIIILVPWA
YLEFGGKKRG

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory