GapMind for catabolism of small carbon sources

 

Protein PP_3284 in Pseudomonas putida KT2440

Annotation: PP_3284 enoyl-CoA hydratase-isomerase

Length: 257 amino acids

Source: Putida in FitnessBrowser

Candidate for 27 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism paaF hi 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 79% 100% 385.6 short chain enoyl-CoA hydratase subunit (EC 4.2.1.17) 53% 236.1
L-isoleucine catabolism ech hi 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 79% 100% 385.6 AcuK 46% 208.0
phenylacetate catabolism paaF hi 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 79% 100% 385.6 short chain enoyl-CoA hydratase subunit (EC 4.2.1.17) 53% 236.1
L-phenylalanine catabolism paaF hi 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 79% 100% 385.6 short chain enoyl-CoA hydratase subunit (EC 4.2.1.17) 53% 236.1
4-hydroxybenzoate catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
L-arginine catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
L-citrulline catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
L-lysine catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
phenylacetate catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
L-phenylalanine catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
L-proline catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
L-valine catabolism ech hi Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized) 51% 97% 231.9 AcuK 46% 208.0
4-hydroxybenzoate catabolism badK med BadK (characterized) 44% 95% 186 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
phenylacetate catabolism badK med BadK (characterized) 44% 95% 186 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-phenylalanine catabolism badK med BadK (characterized) 44% 95% 186 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-isoleucine catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 42% 94% 185.7 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
propionate catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 42% 94% 185.7 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-threonine catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 42% 94% 185.7 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-valine catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 42% 94% 185.7 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-leucine catabolism liuC lo methylglutaconyl-CoA hydratase (EC 4.2.1.18) (characterized) 37% 87% 161.8 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
phenylacetate catabolism paaG lo 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 37% 98% 149.8 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-phenylalanine catabolism paaG lo 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 37% 98% 149.8 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
phenylacetate catabolism paaZ1 lo Enoyl-CoA hydratase; EC 4.2.1.17 (characterized, see rationale) 32% 92% 120.2 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-phenylalanine catabolism paaZ1 lo Enoyl-CoA hydratase; EC 4.2.1.17 (characterized, see rationale) 32% 92% 120.2 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
4-hydroxybenzoate catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase (EC 4.2.1.100) (characterized) 34% 99% 119.8 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
phenylacetate catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase (EC 4.2.1.100) (characterized) 34% 99% 119.8 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6
L-phenylalanine catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase (EC 4.2.1.100) (characterized) 34% 99% 119.8 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) 79% 385.6

Sequence Analysis Tools

View PP_3284 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MPRYIDVQAPEHGVQLITLQRPEALNALCTELLAELAAALQAAGNDEHVRATVITGSAKA
FAAGADIREMADRDLVGILNDPRVAHWQSIAAFAKPLIAAVNGYALGGGCELAMCADIVI
ASTDARFGQPEINLGIIPGAGGTQRLLRAVGKPLAMQMVLTGEAITALRAQQAGLVSEIT
QPELTVERAMQVARSIAAKAPLAVRLAKEALLKAGDTDLASGLRFERHAFTLLAGTADRD
EGIRAFQEKRQARFQGR

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory