GapMind for catabolism of small carbon sources

 

Protein PP_3646 in Pseudomonas putida KT2440

Annotation: PP_3646 Aldehyde dehydrogenase family protein

Length: 493 amino acids

Source: Putida in FitnessBrowser

Candidate for 16 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-phenylalanine catabolism pad-dh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 92% 415.2 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-fucose catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 42% 95% 369.4 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-rhamnose catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 42% 95% 369.4 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-threonine catabolism aldA med NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 42% 95% 369.4 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-arginine catabolism davD med Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 41% 97% 357.8 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-citrulline catabolism davD med Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 41% 97% 357.8 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-lysine catabolism davD med Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 41% 97% 357.8 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-proline catabolism davD med Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 41% 97% 357.8 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-tryptophan catabolism nbaE lo 2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 (characterized) 38% 99% 327.8 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-isoleucine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 32% 94% 250 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
myo-inositol catabolism mmsA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 32% 94% 250 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
propionate catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 32% 94% 250 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-threonine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 32% 94% 250 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-valine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 32% 94% 250 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-valine catabolism mmsA lo malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 34% 96% 249.2 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8
L-lysine catabolism amaB lo Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase; EC 1.2.1.31; EC 1.2.1.3; EC 1.2.1.8 (characterized) 32% 88% 223.8 (Z)-2-((N-methylformamido)methylene)-5-hydroxybutyrolactone dehydrogenase; MFMB dehydrogenase; EC 1.2.1.- 53% 530.8

Sequence Analysis Tools

View PP_3646 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MTLVRFQMCIDGQWRDAQSGKTFDSLNPATAQAWAQLPDADEADVELAVQAAQRAFDSKA
WRSITATARGKLLRRLGDLIAENKEHLAQLESRDNGKLIRETRGQVGYLPEFFHYTAGLA
DKLEGGTLPLDKPDLFAYTVHEPIGVVAGIIPWNSPLYLTAIKLAPALAAGNTIVLKPSE
HASATILELARLALEAGFPAGVVNVVTGYGPSTGAALTRHPLVRKIAFTGGAATARHVVR
SSAENFAKLSLELGGKSPNIIFADADLDSAINGAVAGIYAASGQSCVAGSRLLVQDEIFD
EFVERLIARAKRIRIGNPQDDASEMGPMATAQQLAVVEGLVAAAKAEGAKLHMGGKRADV
EGDGWFYEPTLFECDSNAMTIMQEEVFGPVAAVIRFKTEEEALAMANDSQFGLAAGIWTR
DLGRAHRLARDVRSGIIWVNTYRAVSAMAPIGGFKNSGYGRESGIDSVLAYTELKTVWIN
LSTAPMPDPFVMR

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory