GapMind for catabolism of small carbon sources

 

Protein Synpcc7942_0489 in Synechococcus elongatus PCC 7942

Annotation: Synpcc7942_0489 aldehyde dehydrogenase

Length: 459 amino acids

Source: SynE in FitnessBrowser

Candidate for 25 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism adh med Putative aldehyde dehydrogenase YwdH; EC 1.2.1.3 (uncharacterized) 49% 97% 425.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
2'-deoxyinosine catabolism adh med Putative aldehyde dehydrogenase YwdH; EC 1.2.1.3 (uncharacterized) 49% 97% 425.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
2-deoxy-D-ribose catabolism adh med Putative aldehyde dehydrogenase YwdH; EC 1.2.1.3 (uncharacterized) 49% 97% 425.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
ethanol catabolism adh med Putative aldehyde dehydrogenase YwdH; EC 1.2.1.3 (uncharacterized) 49% 97% 425.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-threonine catabolism adh med Putative aldehyde dehydrogenase YwdH; EC 1.2.1.3 (uncharacterized) 49% 97% 425.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
thymidine catabolism adh med Putative aldehyde dehydrogenase YwdH; EC 1.2.1.3 (uncharacterized) 49% 97% 425.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-tryptophan catabolism adh med Putative aldehyde dehydrogenase YwdH; EC 1.2.1.3 (uncharacterized) 49% 97% 425.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-phenylalanine catabolism pad-dh med long-chain-aldehyde dehydrogenase (EC 1.2.1.48) (characterized) 47% 90% 403.3 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-fucose catabolism aldA lo NAD+-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 33% 94% 186.8 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-rhamnose catabolism aldA lo NAD+-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 33% 94% 186.8 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-threonine catabolism aldA lo NAD+-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 33% 94% 186.8 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-arginine catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19); betaine-aldehyde dehydrogenase (EC 1.2.1.8) (characterized) 34% 83% 182.2 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-citrulline catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19); betaine-aldehyde dehydrogenase (EC 1.2.1.8) (characterized) 34% 83% 182.2 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
putrescine catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19); betaine-aldehyde dehydrogenase (EC 1.2.1.8) (characterized) 34% 83% 182.2 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-arginine catabolism gabD lo Succinate-semialdehyde dehydrogenase, mitochondrial; At-SSADH1; Aldehyde dehydrogenase family 5 member F1; NAD(+)-dependent succinic semialdehyde dehydrogenase; Protein ENLARGED FIL EXPRESSING DOMAIN 1; EC 1.2.1.24 (characterized) 32% 76% 175.3 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-citrulline catabolism gabD lo Succinate-semialdehyde dehydrogenase, mitochondrial; At-SSADH1; Aldehyde dehydrogenase family 5 member F1; NAD(+)-dependent succinic semialdehyde dehydrogenase; Protein ENLARGED FIL EXPRESSING DOMAIN 1; EC 1.2.1.24 (characterized) 32% 76% 175.3 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
putrescine catabolism gabD lo Succinate-semialdehyde dehydrogenase, mitochondrial; At-SSADH1; Aldehyde dehydrogenase family 5 member F1; NAD(+)-dependent succinic semialdehyde dehydrogenase; Protein ENLARGED FIL EXPRESSING DOMAIN 1; EC 1.2.1.24 (characterized) 32% 76% 175.3 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-arginine catabolism kauB lo 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 31% 89% 156.8 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-arginine catabolism puuC lo 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 31% 89% 156.8 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-citrulline catabolism puuC lo 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 31% 89% 156.8 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
putrescine catabolism puuC lo 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 31% 89% 156.8 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
4-hydroxybenzoate catabolism praB lo 2-hydroxymuconic semialdehyde dehydrogenase; HMSD; EC 1.2.1.85 (characterized) 32% 71% 147.5 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-tryptophan catabolism praB lo 2-hydroxymuconic semialdehyde dehydrogenase; HMSD; EC 1.2.1.85 (characterized) 32% 71% 147.5 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-arginine catabolism astD lo N-succinylglutamate 5-semialdehyde dehydrogenase; Succinylglutamic semialdehyde dehydrogenase; SGSD; EC 1.2.1.71 (characterized) 30% 84% 145.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7
L-citrulline catabolism astD lo N-succinylglutamate 5-semialdehyde dehydrogenase; Succinylglutamic semialdehyde dehydrogenase; SGSD; EC 1.2.1.71 (characterized) 30% 84% 145.6 aldehyde dehydrogenase [NAD(P)+] (EC 1.2.1.5) 50% 428.7

Sequence Analysis Tools

View Synpcc7942_0489 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MTAVVLPAAAETLAALQATFDRGDTRTLAFRLARLQDLAKLVADNEAELLQALASDLRKP
ALEAYASEIYFVRDQIKLTCKHLRRWMQPEKQSISLMQQPGQAYRQAEPLGVVLIIGPWN
YPFQLLITPLIGAIAAGNCAVLKPSELAPATSSLIQRLISDRFDPDYIRVLEGDASVSQA
LITQPFDHIFFTGGTAIGRKVMAAAAENLTPVTLELGGKSPCIVDTDIDLDVAARRIAWG
KFFNAGQTCIAPDYLLVQRTVAEPFIEALIDNIQQFYGEDPQQSADYARIVSDRHWQRLN
SLLVDGTIRHGGQVDRSDRYIAPTLITDVNWRDPILQEEIFGPLLPILIYDQLDEAIAQI
RAQPKPLALYLFSRDRQVQERVLAETSAGSVCLNDTILQVGVPDAAFGGVGPSGMGGYHG
KASFETFSHYKLVLKRPFWLDLALRYPPYGDKINLFRKL

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory